Experiment: Azelaic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22670 and RR42_RS22675 are separated by 138 nucleotides RR42_RS22675 and RR42_RS22680 are separated by 180 nucleotides RR42_RS22680 and RR42_RS22685 are separated by 9 nucleotides
RR42_RS22670: RR42_RS22670 - penicillin acylase, at 419,687 to 422,188
_RS22670
RR42_RS22675: RR42_RS22675 - membrane protein, at 422,327 to 423,418
_RS22675
RR42_RS22680: RR42_RS22680 - enoyl-CoA hydratase, at 423,599 to 424,375
_RS22680
RR42_RS22685: RR42_RS22685 - ABC transporter substrate-binding protein, at 424,385 to 425,356
_RS22685
Position (kb) 422
423
424 Strain fitness (log2 ratio) -2
-1
0
1 at 421.475 kb on - strand, within RR42_RS22670 at 421.622 kb on + strand, within RR42_RS22670 at 421.622 kb on + strand, within RR42_RS22670 at 421.623 kb on - strand, within RR42_RS22670 at 421.623 kb on - strand, within RR42_RS22670 at 421.623 kb on - strand, within RR42_RS22670 at 421.700 kb on + strand, within RR42_RS22670 at 421.701 kb on - strand, within RR42_RS22670 at 422.072 kb on + strand at 422.072 kb on + strand at 422.094 kb on - strand at 422.095 kb on + strand at 422.095 kb on + strand at 422.095 kb on + strand at 422.096 kb on - strand at 422.186 kb on + strand at 422.493 kb on + strand, within RR42_RS22675 at 422.494 kb on - strand, within RR42_RS22675 at 422.521 kb on - strand, within RR42_RS22675 at 422.521 kb on - strand, within RR42_RS22675 at 422.521 kb on - strand, within RR42_RS22675 at 422.521 kb on - strand, within RR42_RS22675 at 422.577 kb on + strand, within RR42_RS22675 at 422.577 kb on + strand, within RR42_RS22675 at 422.578 kb on - strand, within RR42_RS22675 at 422.578 kb on - strand, within RR42_RS22675 at 422.580 kb on + strand, within RR42_RS22675 at 422.580 kb on + strand, within RR42_RS22675 at 422.580 kb on + strand, within RR42_RS22675 at 422.679 kb on + strand, within RR42_RS22675 at 422.680 kb on - strand, within RR42_RS22675 at 422.682 kb on + strand, within RR42_RS22675 at 422.683 kb on - strand, within RR42_RS22675 at 422.721 kb on + strand, within RR42_RS22675 at 422.722 kb on - strand, within RR42_RS22675 at 422.722 kb on - strand, within RR42_RS22675 at 422.826 kb on + strand, within RR42_RS22675 at 422.910 kb on + strand, within RR42_RS22675 at 422.910 kb on + strand, within RR42_RS22675 at 422.925 kb on + strand, within RR42_RS22675 at 422.925 kb on + strand, within RR42_RS22675 at 423.051 kb on + strand, within RR42_RS22675 at 423.052 kb on - strand, within RR42_RS22675 at 423.183 kb on - strand, within RR42_RS22675 at 423.188 kb on + strand, within RR42_RS22675 at 423.188 kb on + strand, within RR42_RS22675 at 423.316 kb on - strand at 423.433 kb on + strand at 423.481 kb on + strand at 423.482 kb on - strand at 423.489 kb on + strand at 423.490 kb on - strand at 423.490 kb on - strand at 423.579 kb on + strand at 423.579 kb on + strand at 423.579 kb on + strand at 423.586 kb on + strand at 423.600 kb on + strand at 424.174 kb on + strand, within RR42_RS22680 at 424.360 kb on + strand at 424.408 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Azelaic (C) remove 421,475 - RR42_RS22670 0.71 +0.5 421,622 + RR42_RS22670 0.77 -0.3 421,622 + RR42_RS22670 0.77 +0.1 421,623 - RR42_RS22670 0.77 -1.2 421,623 - RR42_RS22670 0.77 +0.3 421,623 - RR42_RS22670 0.77 -0.8 421,700 + RR42_RS22670 0.80 -0.5 421,701 - RR42_RS22670 0.80 +0.8 422,072 + +0.1 422,072 + +1.1 422,094 - -0.4 422,095 + -0.5 422,095 + +0.1 422,095 + -1.1 422,096 - +0.3 422,186 + +0.1 422,493 + RR42_RS22675 0.15 +0.3 422,494 - RR42_RS22675 0.15 +0.9 422,521 - RR42_RS22675 0.18 +0.3 422,521 - RR42_RS22675 0.18 +0.4 422,521 - RR42_RS22675 0.18 -0.2 422,521 - RR42_RS22675 0.18 -0.1 422,577 + RR42_RS22675 0.23 +0.7 422,577 + RR42_RS22675 0.23 -0.3 422,578 - RR42_RS22675 0.23 -0.6 422,578 - RR42_RS22675 0.23 +0.4 422,580 + RR42_RS22675 0.23 -1.1 422,580 + RR42_RS22675 0.23 -0.3 422,580 + RR42_RS22675 0.23 +0.3 422,679 + RR42_RS22675 0.32 +0.5 422,680 - RR42_RS22675 0.32 -0.1 422,682 + RR42_RS22675 0.33 +0.3 422,683 - RR42_RS22675 0.33 -1.2 422,721 + RR42_RS22675 0.36 -0.4 422,722 - RR42_RS22675 0.36 +0.3 422,722 - RR42_RS22675 0.36 +1.1 422,826 + RR42_RS22675 0.46 +0.3 422,910 + RR42_RS22675 0.53 +0.1 422,910 + RR42_RS22675 0.53 +0.7 422,925 + RR42_RS22675 0.55 +0.4 422,925 + RR42_RS22675 0.55 +0.1 423,051 + RR42_RS22675 0.66 -0.2 423,052 - RR42_RS22675 0.66 +1.1 423,183 - RR42_RS22675 0.78 +0.2 423,188 + RR42_RS22675 0.79 -1.8 423,188 + RR42_RS22675 0.79 -2.2 423,316 - +0.1 423,433 + +0.3 423,481 + -0.2 423,482 - -0.4 423,489 + +0.6 423,490 - -0.5 423,490 - +0.0 423,579 + -0.6 423,579 + -0.8 423,579 + +1.7 423,586 + -0.1 423,600 + -0.4 424,174 + RR42_RS22680 0.74 -1.0 424,360 + -0.9 424,408 + -0.7
Or see this region's nucleotide sequence