Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23950 and BWI76_RS23955 overlap by 4 nucleotides BWI76_RS23955 and BWI76_RS23960 are separated by 121 nucleotides BWI76_RS23960 and BWI76_RS23965 are separated by 90 nucleotides
BWI76_RS23950: BWI76_RS23950 - succinate CoA transferase, at 4,998,900 to 5,000,366
_RS23950
BWI76_RS23955: BWI76_RS23955 - LysR family transcriptional regulator, at 5,000,363 to 5,001,259
_RS23955
BWI76_RS23960: BWI76_RS23960 - oxidative stress defense protein, at 5,001,381 to 5,002,148
_RS23960
BWI76_RS23965: BWI76_RS23965 - arginine exporter protein, at 5,002,239 to 5,002,874
_RS23965
Position (kb) 5000
5001
5002 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 4999.466 kb on + strand, within BWI76_RS23950 at 4999.603 kb on + strand, within BWI76_RS23950 at 4999.746 kb on - strand, within BWI76_RS23950 at 4999.746 kb on - strand, within BWI76_RS23950 at 4999.850 kb on - strand, within BWI76_RS23950 at 4999.873 kb on + strand, within BWI76_RS23950 at 4999.932 kb on - strand, within BWI76_RS23950 at 4999.980 kb on + strand, within BWI76_RS23950 at 4999.999 kb on + strand, within BWI76_RS23950 at 5000.007 kb on - strand, within BWI76_RS23950 at 5000.146 kb on + strand, within BWI76_RS23950 at 5000.199 kb on - strand, within BWI76_RS23950 at 5000.350 kb on + strand at 5000.350 kb on + strand at 5000.533 kb on - strand, within BWI76_RS23955 at 5000.579 kb on + strand, within BWI76_RS23955 at 5000.624 kb on + strand, within BWI76_RS23955 at 5000.851 kb on + strand, within BWI76_RS23955 at 5000.969 kb on + strand, within BWI76_RS23955 at 5001.080 kb on - strand, within BWI76_RS23955 at 5001.112 kb on + strand, within BWI76_RS23955 at 5001.474 kb on - strand, within BWI76_RS23960 at 5001.603 kb on - strand, within BWI76_RS23960 at 5001.644 kb on - strand, within BWI76_RS23960 at 5001.702 kb on - strand, within BWI76_RS23960 at 5001.769 kb on + strand, within BWI76_RS23960 at 5001.873 kb on - strand, within BWI76_RS23960 at 5001.936 kb on - strand, within BWI76_RS23960 at 5002.184 kb on - strand at 5002.252 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x remove 4,999,466 + BWI76_RS23950 0.39 -0.1 4,999,603 + BWI76_RS23950 0.48 +0.9 4,999,746 - BWI76_RS23950 0.58 -1.3 4,999,746 - BWI76_RS23950 0.58 -1.3 4,999,850 - BWI76_RS23950 0.65 +0.8 4,999,873 + BWI76_RS23950 0.66 +1.3 4,999,932 - BWI76_RS23950 0.70 -0.2 4,999,980 + BWI76_RS23950 0.74 +0.1 4,999,999 + BWI76_RS23950 0.75 -0.1 5,000,007 - BWI76_RS23950 0.75 -0.4 5,000,146 + BWI76_RS23950 0.85 -1.5 5,000,199 - BWI76_RS23950 0.89 +0.0 5,000,350 + +0.1 5,000,350 + -1.7 5,000,533 - BWI76_RS23955 0.19 +4.6 5,000,579 + BWI76_RS23955 0.24 -0.9 5,000,624 + BWI76_RS23955 0.29 -0.6 5,000,851 + BWI76_RS23955 0.54 +0.9 5,000,969 + BWI76_RS23955 0.68 +4.0 5,001,080 - BWI76_RS23955 0.80 -0.0 5,001,112 + BWI76_RS23955 0.84 -0.2 5,001,474 - BWI76_RS23960 0.12 -1.0 5,001,603 - BWI76_RS23960 0.29 -0.2 5,001,644 - BWI76_RS23960 0.34 -0.2 5,001,702 - BWI76_RS23960 0.42 -1.2 5,001,769 + BWI76_RS23960 0.51 -0.8 5,001,873 - BWI76_RS23960 0.64 +1.4 5,001,936 - BWI76_RS23960 0.72 +0.3 5,002,184 - +0.5 5,002,252 + +3.1
Or see this region's nucleotide sequence