Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18130 and BWI76_RS18135 are separated by 78 nucleotides BWI76_RS18135 and BWI76_RS18140 overlap by 1 nucleotides BWI76_RS18140 and BWI76_RS18145 are separated by 198 nucleotides
BWI76_RS18130: BWI76_RS18130 - zinc ABC transporter substrate-binding protein, at 3,768,651 to 3,769,595
_RS18130
BWI76_RS18135: BWI76_RS18135 - zinc ABC transporter ATP-binding protein ZnuC, at 3,769,674 to 3,770,426
_RS18135
BWI76_RS18140: BWI76_RS18140 - zinc ABC transporter permease, at 3,770,426 to 3,771,211
_RS18140
BWI76_RS18145: BWI76_RS18145 - Holliday junction branch migration DNA helicase RuvB, at 3,771,410 to 3,772,420
_RS18145
Position (kb)
3770
3771
3772 Strain fitness (log2 ratio)
-2
-1
0
1 at 3769.758 kb on - strand, within BWI76_RS18135 at 3769.758 kb on - strand, within BWI76_RS18135 at 3769.778 kb on + strand, within BWI76_RS18135 at 3769.890 kb on - strand, within BWI76_RS18135 at 3769.991 kb on - strand, within BWI76_RS18135 at 3770.110 kb on + strand, within BWI76_RS18135 at 3770.122 kb on + strand, within BWI76_RS18135 at 3770.542 kb on - strand, within BWI76_RS18140 at 3770.578 kb on + strand, within BWI76_RS18140 at 3770.611 kb on - strand, within BWI76_RS18140 at 3771.435 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x remove 3,769,758 - BWI76_RS18135 0.11 -0.8 3,769,758 - BWI76_RS18135 0.11 -1.9 3,769,778 + BWI76_RS18135 0.14 -2.0 3,769,890 - BWI76_RS18135 0.29 -2.3 3,769,991 - BWI76_RS18135 0.42 +0.2 3,770,110 + BWI76_RS18135 0.58 -0.7 3,770,122 + BWI76_RS18135 0.59 -1.6 3,770,542 - BWI76_RS18140 0.15 -1.2 3,770,578 + BWI76_RS18140 0.19 -1.5 3,770,611 - BWI76_RS18140 0.24 -0.5 3,771,435 - -0.2
Or see this region's nucleotide sequence