Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10490 and BWI76_RS10495 are separated by 125 nucleotides BWI76_RS10495 and BWI76_RS10500 are separated by 174 nucleotides
BWI76_RS10490: BWI76_RS10490 - vanillate O-demethylase oxygenase subunit, at 2,216,391 to 2,217,428
_RS10490
BWI76_RS10495: BWI76_RS10495 - LysR family transcriptional regulator, at 2,217,554 to 2,218,492
_RS10495
BWI76_RS10500: BWI76_RS10500 - flavoprotein monooxygenase, at 2,218,667 to 2,219,821
_RS10500
Position (kb)
2217
2218
2219 Strain fitness (log2 ratio)
-1
0
1
2 at 2216.560 kb on - strand, within BWI76_RS10490 at 2216.841 kb on - strand, within BWI76_RS10490 at 2216.881 kb on + strand, within BWI76_RS10490 at 2217.254 kb on + strand, within BWI76_RS10490 at 2217.431 kb on + strand at 2217.431 kb on - strand at 2217.915 kb on + strand, within BWI76_RS10495 at 2218.027 kb on - strand, within BWI76_RS10495 at 2218.128 kb on + strand, within BWI76_RS10495 at 2218.303 kb on + strand, within BWI76_RS10495 at 2218.308 kb on + strand, within BWI76_RS10495 at 2218.362 kb on + strand, within BWI76_RS10495 at 2218.367 kb on + strand, within BWI76_RS10495 at 2218.588 kb on - strand at 2218.588 kb on - strand at 2218.700 kb on - strand at 2218.771 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x remove 2,216,560 - BWI76_RS10490 0.16 +1.1 2,216,841 - BWI76_RS10490 0.43 -0.9 2,216,881 + BWI76_RS10490 0.47 -0.8 2,217,254 + BWI76_RS10490 0.83 +0.5 2,217,431 + +0.3 2,217,431 - -0.5 2,217,915 + BWI76_RS10495 0.38 +1.3 2,218,027 - BWI76_RS10495 0.50 +0.8 2,218,128 + BWI76_RS10495 0.61 +0.2 2,218,303 + BWI76_RS10495 0.80 +1.6 2,218,308 + BWI76_RS10495 0.80 +0.3 2,218,362 + BWI76_RS10495 0.86 +1.3 2,218,367 + BWI76_RS10495 0.87 +0.3 2,218,588 - +0.1 2,218,588 - +0.4 2,218,700 - -0.6 2,218,771 - +1.8
Or see this region's nucleotide sequence