Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07715 and BWI76_RS07720 are separated by 85 nucleotides BWI76_RS07720 and BWI76_RS07725 overlap by 4 nucleotides BWI76_RS07725 and BWI76_RS07730 overlap by 4 nucleotides
BWI76_RS07715: BWI76_RS07715 - non-ribosomal peptide synthetase, at 1,633,803 to 1,637,690
_RS07715
BWI76_RS07720: BWI76_RS07720 - iron-enterobactin transporter ATP-binding protein, at 1,637,776 to 1,638,570
_RS07720
BWI76_RS07725: BWI76_RS07725 - iron-enterobactin transporter permease, at 1,638,567 to 1,639,559
_RS07725
BWI76_RS07730: BWI76_RS07730 - iron-enterobactin transporter membrane protein, at 1,639,556 to 1,640,563
_RS07730
Position (kb)
1638
1639
1640 Strain fitness (log2 ratio)
-1
0
1 at 1637.606 kb on + strand at 1637.754 kb on + strand at 1638.416 kb on - strand, within BWI76_RS07720 at 1638.551 kb on + strand at 1638.661 kb on + strand at 1639.293 kb on + strand, within BWI76_RS07725 at 1640.043 kb on + strand, within BWI76_RS07730 at 1640.527 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x remove 1,637,606 + +1.4 1,637,754 + +0.5 1,638,416 - BWI76_RS07720 0.81 -0.7 1,638,551 + -0.5 1,638,661 + -1.4 1,639,293 + BWI76_RS07725 0.73 -0.9 1,640,043 + BWI76_RS07730 0.48 -1.4 1,640,527 + -1.1
Or see this region's nucleotide sequence