Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05125

Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05120 and BWI76_RS05125 are separated by 274 nucleotidesBWI76_RS05125 and BWI76_RS05130 are separated by 45 nucleotidesBWI76_RS05130 and BWI76_RS05135 overlap by 1 nucleotides BWI76_RS05120: BWI76_RS05120 - bifunctional glycosyl transferase/transpeptidase, at 1,073,305 to 1,075,860 _RS05120 BWI76_RS05125: BWI76_RS05125 - ferrichrome porin FhuA, at 1,076,135 to 1,078,339 _RS05125 BWI76_RS05130: BWI76_RS05130 - iron-hydroxamate transporter ATP-binding subunit, at 1,078,385 to 1,079,182 _RS05130 BWI76_RS05135: BWI76_RS05135 - iron-hydroxamate transporter substrate-binding subunit, at 1,079,182 to 1,080,072 _RS05135 Position (kb) 1076 1077 1078 1079Strain fitness (log2 ratio) -2 -1 0 1 2at 1075.288 kb on + strand, within BWI76_RS05120at 1075.428 kb on - strand, within BWI76_RS05120at 1075.498 kb on + strand, within BWI76_RS05120at 1075.511 kb on - strand, within BWI76_RS05120at 1075.604 kb on - strand, within BWI76_RS05120at 1075.659 kb on + strandat 1075.814 kb on - strandat 1075.832 kb on + strandat 1075.840 kb on - strandat 1075.845 kb on + strandat 1075.867 kb on + strandat 1075.867 kb on + strandat 1075.935 kb on + strandat 1075.990 kb on - strandat 1076.047 kb on - strandat 1076.140 kb on - strandat 1076.143 kb on + strandat 1076.143 kb on + strandat 1076.143 kb on + strandat 1076.151 kb on - strandat 1076.151 kb on - strandat 1076.151 kb on - strandat 1076.230 kb on + strandat 1076.281 kb on - strandat 1076.290 kb on - strandat 1076.453 kb on - strand, within BWI76_RS05125at 1076.522 kb on - strand, within BWI76_RS05125at 1076.528 kb on - strand, within BWI76_RS05125at 1076.530 kb on + strand, within BWI76_RS05125at 1076.616 kb on + strand, within BWI76_RS05125at 1076.640 kb on + strand, within BWI76_RS05125at 1076.664 kb on + strand, within BWI76_RS05125at 1076.876 kb on + strand, within BWI76_RS05125at 1076.886 kb on + strand, within BWI76_RS05125at 1077.121 kb on + strand, within BWI76_RS05125at 1077.192 kb on + strand, within BWI76_RS05125at 1077.311 kb on + strand, within BWI76_RS05125at 1077.311 kb on + strand, within BWI76_RS05125at 1077.319 kb on - strand, within BWI76_RS05125at 1077.420 kb on + strand, within BWI76_RS05125at 1077.651 kb on + strand, within BWI76_RS05125at 1077.851 kb on - strand, within BWI76_RS05125at 1078.022 kb on + strand, within BWI76_RS05125at 1078.533 kb on + strand, within BWI76_RS05130at 1078.686 kb on - strand, within BWI76_RS05130at 1078.786 kb on + strand, within BWI76_RS05130at 1078.794 kb on - strand, within BWI76_RS05130at 1078.876 kb on + strand, within BWI76_RS05130at 1078.977 kb on + strand, within BWI76_RS05130at 1079.052 kb on + strand, within BWI76_RS05130

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
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1,075,288 + BWI76_RS05120 0.78 -1.2
1,075,428 - BWI76_RS05120 0.83 -2.1
1,075,498 + BWI76_RS05120 0.86 -0.6
1,075,511 - BWI76_RS05120 0.86 +0.0
1,075,604 - BWI76_RS05120 0.90 -1.1
1,075,659 + -0.1
1,075,814 - +0.1
1,075,832 + -0.7
1,075,840 - +0.5
1,075,845 + -0.1
1,075,867 + -0.6
1,075,867 + -1.8
1,075,935 + -0.4
1,075,990 - -0.1
1,076,047 - -1.6
1,076,140 - +0.6
1,076,143 + +2.1
1,076,143 + -0.2
1,076,143 + -1.1
1,076,151 - -0.6
1,076,151 - -0.9
1,076,151 - -0.8
1,076,230 + +0.1
1,076,281 - +0.7
1,076,290 - -1.2
1,076,453 - BWI76_RS05125 0.14 -2.0
1,076,522 - BWI76_RS05125 0.18 -0.7
1,076,528 - BWI76_RS05125 0.18 -0.6
1,076,530 + BWI76_RS05125 0.18 -0.3
1,076,616 + BWI76_RS05125 0.22 -2.5
1,076,640 + BWI76_RS05125 0.23 -2.1
1,076,664 + BWI76_RS05125 0.24 -0.8
1,076,876 + BWI76_RS05125 0.34 -0.9
1,076,886 + BWI76_RS05125 0.34 -1.1
1,077,121 + BWI76_RS05125 0.45 -0.9
1,077,192 + BWI76_RS05125 0.48 +0.1
1,077,311 + BWI76_RS05125 0.53 -0.8
1,077,311 + BWI76_RS05125 0.53 -1.6
1,077,319 - BWI76_RS05125 0.54 -1.0
1,077,420 + BWI76_RS05125 0.58 +0.3
1,077,651 + BWI76_RS05125 0.69 -0.4
1,077,851 - BWI76_RS05125 0.78 -0.6
1,078,022 + BWI76_RS05125 0.86 -2.0
1,078,533 + BWI76_RS05130 0.19 -0.2
1,078,686 - BWI76_RS05130 0.38 -1.4
1,078,786 + BWI76_RS05130 0.50 -0.6
1,078,794 - BWI76_RS05130 0.51 -0.3
1,078,876 + BWI76_RS05130 0.62 +0.0
1,078,977 + BWI76_RS05130 0.74 +0.9
1,079,052 + BWI76_RS05130 0.84 -0.2

Or see this region's nucleotide sequence