Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05035 and BWI76_RS05040 overlap by 1 nucleotides BWI76_RS05040 and BWI76_RS05045 overlap by 11 nucleotides BWI76_RS05045 and BWI76_RS05050 overlap by 8 nucleotides
BWI76_RS05035: BWI76_RS05035 - prepilin peptidase, at 1,056,453 to 1,057,283
_RS05035
BWI76_RS05040: BWI76_RS05040 - type II secretion system protein N, at 1,057,283 to 1,058,041
_RS05040
BWI76_RS05045: BWI76_RS05045 - general secretion pathway protein GspM, at 1,058,031 to 1,058,516
_RS05045
BWI76_RS05050: BWI76_RS05050 - general secretion pathway protein GspL, at 1,058,509 to 1,059,705
_RS05050
Position (kb)
1058
1059 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1057.096 kb on - strand at 1057.373 kb on + strand, within BWI76_RS05040 at 1057.468 kb on + strand, within BWI76_RS05040 at 1057.486 kb on - strand, within BWI76_RS05040 at 1057.520 kb on + strand, within BWI76_RS05040 at 1057.946 kb on - strand, within BWI76_RS05040 at 1058.457 kb on - strand, within BWI76_RS05045 at 1058.457 kb on - strand, within BWI76_RS05045 at 1058.541 kb on + strand at 1058.616 kb on + strand at 1058.624 kb on - strand at 1058.624 kb on - strand at 1059.018 kb on + strand, within BWI76_RS05050 at 1059.306 kb on + strand, within BWI76_RS05050 at 1059.306 kb on + strand, within BWI76_RS05050
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x remove 1,057,096 - +0.9 1,057,373 + BWI76_RS05040 0.12 -0.7 1,057,468 + BWI76_RS05040 0.24 +0.9 1,057,486 - BWI76_RS05040 0.27 +1.1 1,057,520 + BWI76_RS05040 0.31 -2.7 1,057,946 - BWI76_RS05040 0.87 +0.3 1,058,457 - BWI76_RS05045 0.88 -2.0 1,058,457 - BWI76_RS05045 0.88 -1.3 1,058,541 + +0.9 1,058,616 + +2.9 1,058,624 - +1.4 1,058,624 - -0.1 1,059,018 + BWI76_RS05050 0.43 +0.3 1,059,306 + BWI76_RS05050 0.67 +1.0 1,059,306 + BWI76_RS05050 0.67 -1.1
Or see this region's nucleotide sequence