Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02185 and BWI76_RS02190 are separated by 14 nucleotides BWI76_RS02190 and BWI76_RS02195 overlap by 1 nucleotides BWI76_RS02195 and BWI76_RS02200 overlap by 4 nucleotides BWI76_RS02200 and BWI76_RS02205 are separated by 196 nucleotides
BWI76_RS02185: BWI76_RS02185 - phosphonate metabolism protein PhnP, at 453,409 to 454,167
_RS02185
BWI76_RS02190: BWI76_RS02190 - ribose 1,5-bisphosphokinase, at 454,182 to 454,745
_RS02190
BWI76_RS02195: BWI76_RS02195 - alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase, at 454,745 to 455,881
_RS02195
BWI76_RS02200: BWI76_RS02200 - phosphonate C-P lyase system protein PhnL, at 455,878 to 456,564
_RS02200
BWI76_RS02205: BWI76_RS02205 - phosphonate C-P lyase system protein PhnK, at 456,761 to 457,519
_RS02205
Position (kb) 454
455
456 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 453.803 kb on - strand, within BWI76_RS02185 at 453.803 kb on - strand, within BWI76_RS02185 at 453.830 kb on + strand, within BWI76_RS02185 at 453.848 kb on - strand, within BWI76_RS02185 at 453.943 kb on + strand, within BWI76_RS02185 at 454.169 kb on - strand at 454.274 kb on + strand, within BWI76_RS02190 at 454.343 kb on + strand, within BWI76_RS02190 at 454.559 kb on + strand, within BWI76_RS02190 at 454.695 kb on + strand at 454.695 kb on + strand at 454.695 kb on + strand at 454.695 kb on + strand at 454.763 kb on - strand at 454.797 kb on + strand at 454.920 kb on - strand at 454.931 kb on + strand, within BWI76_RS02195 at 455.083 kb on - strand, within BWI76_RS02195 at 455.091 kb on - strand, within BWI76_RS02195 at 455.298 kb on - strand, within BWI76_RS02195 at 455.367 kb on + strand, within BWI76_RS02195 at 455.479 kb on + strand, within BWI76_RS02195 at 455.618 kb on - strand, within BWI76_RS02195 at 455.656 kb on - strand, within BWI76_RS02195 at 455.667 kb on + strand, within BWI76_RS02195 at 455.680 kb on - strand, within BWI76_RS02195 at 455.860 kb on + strand at 455.860 kb on + strand at 455.868 kb on - strand at 455.904 kb on + strand at 456.015 kb on - strand, within BWI76_RS02200 at 456.016 kb on + strand, within BWI76_RS02200 at 456.268 kb on - strand, within BWI76_RS02200 at 456.309 kb on - strand, within BWI76_RS02200 at 456.316 kb on - strand, within BWI76_RS02200 at 456.316 kb on - strand, within BWI76_RS02200 at 456.326 kb on - strand, within BWI76_RS02200 at 456.439 kb on + strand, within BWI76_RS02200 at 456.648 kb on - strand at 456.716 kb on + strand at 456.724 kb on - strand at 456.747 kb on + strand at 456.795 kb on + strand at 456.795 kb on + strand at 456.803 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x remove 453,803 - BWI76_RS02185 0.52 +0.3 453,803 - BWI76_RS02185 0.52 -1.5 453,830 + BWI76_RS02185 0.55 +0.1 453,848 - BWI76_RS02185 0.58 -1.7 453,943 + BWI76_RS02185 0.70 +0.2 454,169 - +1.6 454,274 + BWI76_RS02190 0.16 -0.7 454,343 + BWI76_RS02190 0.29 -1.7 454,559 + BWI76_RS02190 0.67 -0.0 454,695 + -0.4 454,695 + +0.4 454,695 + +0.5 454,695 + -0.2 454,763 - -1.4 454,797 + +0.4 454,920 - +0.0 454,931 + BWI76_RS02195 0.16 +2.5 455,083 - BWI76_RS02195 0.30 +1.0 455,091 - BWI76_RS02195 0.30 -0.8 455,298 - BWI76_RS02195 0.49 +0.2 455,367 + BWI76_RS02195 0.55 +0.5 455,479 + BWI76_RS02195 0.65 +0.7 455,618 - BWI76_RS02195 0.77 -0.3 455,656 - BWI76_RS02195 0.80 +4.0 455,667 + BWI76_RS02195 0.81 -0.9 455,680 - BWI76_RS02195 0.82 +1.8 455,860 + -0.1 455,860 + +0.8 455,868 - +0.5 455,904 + -1.1 456,015 - BWI76_RS02200 0.20 -0.2 456,016 + BWI76_RS02200 0.20 +0.0 456,268 - BWI76_RS02200 0.57 +1.5 456,309 - BWI76_RS02200 0.63 +0.8 456,316 - BWI76_RS02200 0.64 +1.3 456,316 - BWI76_RS02200 0.64 +0.5 456,326 - BWI76_RS02200 0.65 -0.6 456,439 + BWI76_RS02200 0.82 +0.5 456,648 - +0.6 456,716 + -1.2 456,724 - +0.3 456,747 + +0.3 456,795 + -0.4 456,795 + +0.2 456,803 - -0.1
Or see this region's nucleotide sequence