Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01780 and BWI76_RS01785 are separated by 5 nucleotides BWI76_RS01785 and BWI76_RS01790 are separated by 38 nucleotides BWI76_RS01790 and BWI76_RS01795 are separated by 121 nucleotides BWI76_RS01795 and BWI76_RS01800 are separated by 282 nucleotides
BWI76_RS01780: BWI76_RS01780 - SPFH domain / Band 7 family protein, at 363,171 to 364,298
_RS01780
BWI76_RS01785: BWI76_RS01785 - nucleotidyltransferase, at 364,304 to 365,092
_RS01785
BWI76_RS01790: BWI76_RS01790 - lysine-sensitive aspartokinase 3, at 365,131 to 366,480
_RS01790
BWI76_RS01795: BWI76_RS01795 - hypothetical protein, at 366,602 to 366,964
_RS01795
BWI76_RS01800: BWI76_RS01800 - glucose-6-phosphate isomerase, at 367,247 to 368,896
_RS01800
Position (kb) 365
366
367 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 364.145 kb on + strand, within BWI76_RS01780 at 364.211 kb on + strand at 364.216 kb on + strand at 364.216 kb on + strand at 364.454 kb on - strand, within BWI76_RS01785 at 364.515 kb on + strand, within BWI76_RS01785 at 364.854 kb on + strand, within BWI76_RS01785 at 364.923 kb on - strand, within BWI76_RS01785 at 364.928 kb on - strand, within BWI76_RS01785 at 364.986 kb on + strand, within BWI76_RS01785 at 364.996 kb on + strand, within BWI76_RS01785 at 365.004 kb on - strand, within BWI76_RS01785 at 365.065 kb on + strand at 365.273 kb on - strand, within BWI76_RS01790 at 365.344 kb on - strand, within BWI76_RS01790 at 365.381 kb on - strand, within BWI76_RS01790 at 365.450 kb on - strand, within BWI76_RS01790 at 365.481 kb on - strand, within BWI76_RS01790 at 365.539 kb on + strand, within BWI76_RS01790 at 365.547 kb on - strand, within BWI76_RS01790 at 365.661 kb on + strand, within BWI76_RS01790 at 365.689 kb on - strand, within BWI76_RS01790 at 365.694 kb on - strand, within BWI76_RS01790 at 365.790 kb on + strand, within BWI76_RS01790 at 365.860 kb on - strand, within BWI76_RS01790 at 365.908 kb on + strand, within BWI76_RS01790 at 365.916 kb on - strand, within BWI76_RS01790 at 365.916 kb on - strand, within BWI76_RS01790 at 366.119 kb on - strand, within BWI76_RS01790 at 366.166 kb on + strand, within BWI76_RS01790 at 366.234 kb on + strand, within BWI76_RS01790 at 366.242 kb on - strand, within BWI76_RS01790 at 366.253 kb on + strand, within BWI76_RS01790 at 366.418 kb on + strand at 366.663 kb on + strand, within BWI76_RS01795 at 366.681 kb on + strand, within BWI76_RS01795 at 366.801 kb on + strand, within BWI76_RS01795 at 366.931 kb on + strand at 366.938 kb on - strand at 366.990 kb on - strand at 367.238 kb on + strand at 367.265 kb on + strand at 367.443 kb on - strand, within BWI76_RS01800
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x remove 364,145 + BWI76_RS01780 0.86 +0.2 364,211 + -1.4 364,216 + +0.2 364,216 + +1.1 364,454 - BWI76_RS01785 0.19 +0.1 364,515 + BWI76_RS01785 0.27 +3.9 364,854 + BWI76_RS01785 0.70 +0.5 364,923 - BWI76_RS01785 0.78 +0.1 364,928 - BWI76_RS01785 0.79 -0.1 364,986 + BWI76_RS01785 0.86 -0.3 364,996 + BWI76_RS01785 0.88 -0.8 365,004 - BWI76_RS01785 0.89 +1.0 365,065 + +0.1 365,273 - BWI76_RS01790 0.11 -0.9 365,344 - BWI76_RS01790 0.16 -2.1 365,381 - BWI76_RS01790 0.19 -0.6 365,450 - BWI76_RS01790 0.24 -0.1 365,481 - BWI76_RS01790 0.26 -1.0 365,539 + BWI76_RS01790 0.30 -1.5 365,547 - BWI76_RS01790 0.31 -2.0 365,661 + BWI76_RS01790 0.39 -1.6 365,689 - BWI76_RS01790 0.41 -2.3 365,694 - BWI76_RS01790 0.42 -0.6 365,790 + BWI76_RS01790 0.49 -1.1 365,860 - BWI76_RS01790 0.54 +0.1 365,908 + BWI76_RS01790 0.58 -0.5 365,916 - BWI76_RS01790 0.58 -0.9 365,916 - BWI76_RS01790 0.58 -1.3 366,119 - BWI76_RS01790 0.73 -0.8 366,166 + BWI76_RS01790 0.77 -0.7 366,234 + BWI76_RS01790 0.82 -0.3 366,242 - BWI76_RS01790 0.82 -2.2 366,253 + BWI76_RS01790 0.83 -0.7 366,418 + -1.9 366,663 + BWI76_RS01795 0.17 -1.4 366,681 + BWI76_RS01795 0.22 -0.7 366,801 + BWI76_RS01795 0.55 -0.5 366,931 + -0.5 366,938 - +2.8 366,990 - -0.5 367,238 + +0.4 367,265 + -0.0 367,443 - BWI76_RS01800 0.12 +1.5
Or see this region's nucleotide sequence