Experiment: Pimelic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10615 and RR42_RS10620 are separated by 9 nucleotides RR42_RS10620 and RR42_RS10625 are separated by 42 nucleotides RR42_RS10625 and RR42_RS10630 are separated by 29 nucleotides RR42_RS10630 and RR42_RS10635 are separated by 134 nucleotides
RR42_RS10615: RR42_RS10615 - 2-hydroxyacid dehydrogenase, at 2,267,898 to 2,268,923
_RS10615
RR42_RS10620: RR42_RS10620 - amidohydrolase, at 2,268,933 to 2,269,763
_RS10620
RR42_RS10625: RR42_RS10625 - glucarate dehydratase, at 2,269,806 to 2,271,164
_RS10625
RR42_RS10630: RR42_RS10630 - hypothetical protein, at 2,271,194 to 2,271,544
_RS10630
RR42_RS10635: RR42_RS10635 - hypothetical protein, at 2,271,679 to 2,272,431
_RS10635
Position (kb)
2269
2270
2271
2272 Strain fitness (log2 ratio)
-1
0
1 at 2268.921 kb on + strand at 2269.003 kb on - strand at 2269.255 kb on - strand, within RR42_RS10620 at 2269.255 kb on - strand, within RR42_RS10620 at 2269.348 kb on + strand, within RR42_RS10620 at 2269.521 kb on + strand, within RR42_RS10620 at 2269.594 kb on - strand, within RR42_RS10620 at 2269.717 kb on + strand at 2269.717 kb on + strand at 2269.727 kb on + strand at 2269.743 kb on + strand at 2269.743 kb on + strand at 2269.744 kb on - strand at 2269.826 kb on + strand at 2269.826 kb on + strand at 2271.654 kb on + strand at 2271.687 kb on + strand at 2271.823 kb on + strand, within RR42_RS10635 at 2271.823 kb on + strand, within RR42_RS10635 at 2271.823 kb on + strand, within RR42_RS10635 at 2271.824 kb on - strand, within RR42_RS10635 at 2271.925 kb on - strand, within RR42_RS10635
Per-strain Table
Position Strand Gene LocusTag Fraction Pimelic (C) remove 2,268,921 + -0.8 2,269,003 - -0.0 2,269,255 - RR42_RS10620 0.39 -0.1 2,269,255 - RR42_RS10620 0.39 +0.0 2,269,348 + RR42_RS10620 0.50 -0.5 2,269,521 + RR42_RS10620 0.71 +0.1 2,269,594 - RR42_RS10620 0.80 +0.2 2,269,717 + +0.2 2,269,717 + -0.4 2,269,727 + +0.6 2,269,743 + -1.0 2,269,743 + +0.7 2,269,744 - -0.6 2,269,826 + -0.6 2,269,826 + -0.4 2,271,654 + -0.6 2,271,687 + +1.7 2,271,823 + RR42_RS10635 0.19 +0.7 2,271,823 + RR42_RS10635 0.19 -0.3 2,271,823 + RR42_RS10635 0.19 -0.0 2,271,824 - RR42_RS10635 0.19 -0.2 2,271,925 - RR42_RS10635 0.33 -0.7
Or see this region's nucleotide sequence