Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4561 and BT4562 are separated by 6 nucleotides BT4562 and BT4563 are separated by 163 nucleotides BT4563 and BT4564 are separated by 20 nucleotides BT4564 and BT4565 are separated by 23 nucleotides
BT4561: BT4561 - conserved hypothetical protein, putative integral membrane protein (NCBI ptt file), at 5,982,671 to 5,983,252
BT4561
BT4562: BT4562 - hypothetical protein (NCBI ptt file), at 5,983,259 to 5,983,825
BT4562
BT4563: BT4563 - hypothetical protein (NCBI ptt file), at 5,983,989 to 5,984,540
BT4563
BT4564: BT4564 - putative glycosyltransferase (NCBI ptt file), at 5,984,561 to 5,985,511
BT4564
BT4565: BT4565 - hypothetical protein (NCBI ptt file), at 5,985,535 to 5,985,909
BT4565
Position (kb)
5983
5984
5985 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5983.004 kb on - strand, within BT4561 at 5983.004 kb on - strand, within BT4561 at 5983.050 kb on - strand, within BT4561 at 5983.163 kb on - strand, within BT4561 at 5983.351 kb on - strand, within BT4562 at 5983.389 kb on - strand, within BT4562 at 5983.582 kb on - strand, within BT4562 at 5983.707 kb on - strand, within BT4562 at 5984.199 kb on - strand, within BT4563 at 5984.204 kb on - strand, within BT4563 at 5984.211 kb on - strand, within BT4563 at 5984.426 kb on - strand, within BT4563 at 5984.426 kb on - strand, within BT4563 at 5984.515 kb on - strand at 5984.541 kb on - strand at 5985.523 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 5,983,004 - BT4561 0.57 -1.6 5,983,004 - BT4561 0.57 -1.3 5,983,050 - BT4561 0.65 -1.3 5,983,163 - BT4561 0.85 -3.2 5,983,351 - BT4562 0.16 -1.9 5,983,389 - BT4562 0.23 +1.4 5,983,582 - BT4562 0.57 +0.1 5,983,707 - BT4562 0.79 -2.2 5,984,199 - BT4563 0.38 -3.2 5,984,204 - BT4563 0.39 +1.4 5,984,211 - BT4563 0.40 -0.9 5,984,426 - BT4563 0.79 -2.2 5,984,426 - BT4563 0.79 -1.9 5,984,515 - -1.9 5,984,541 - -1.5 5,985,523 - +1.1
Or see this region's nucleotide sequence