Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3209 and BT3210 are separated by 3 nucleotides BT3210 and BT3211 are separated by 49 nucleotides BT3211 and BT3212 are separated by 12 nucleotides BT3212 and BT3213 are separated by 52 nucleotides
BT3209: BT3209 - conserved hypothetical protein (NCBI ptt file), at 4,102,964 to 4,103,803
BT3209
BT3210: BT3210 - cell division protein FtsX (NCBI ptt file), at 4,103,807 to 4,104,688
BT3210
BT3211: BT3211 - conserved hypothetical protein (NCBI ptt file), at 4,104,738 to 4,104,971
BT3211
BT3212: BT3212 - putative bacitracin resistance protein (NCBI ptt file), at 4,104,984 to 4,105,778
BT3212
BT3213: BT3213 - tRNA pseudouridine synthase B (NCBI ptt file), at 4,105,831 to 4,106,553
BT3213
Position (kb)
4104
4105 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4103.825 kb on + strand at 4103.827 kb on + strand at 4103.827 kb on + strand at 4103.848 kb on + strand at 4103.869 kb on + strand at 4103.935 kb on + strand, within BT3210 at 4103.943 kb on + strand, within BT3210 at 4104.004 kb on + strand, within BT3210 at 4104.234 kb on + strand, within BT3210 at 4104.285 kb on + strand, within BT3210 at 4104.334 kb on + strand, within BT3210 at 4104.936 kb on + strand, within BT3211 at 4104.936 kb on + strand, within BT3211 at 4104.950 kb on + strand at 4105.793 kb on + strand at 4105.898 kb on + strand at 4105.952 kb on + strand, within BT3213
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 4,103,825 + -3.6 4,103,827 + +0.4 4,103,827 + -0.2 4,103,848 + -0.9 4,103,869 + -0.7 4,103,935 + BT3210 0.15 -0.2 4,103,943 + BT3210 0.15 -1.3 4,104,004 + BT3210 0.22 +0.2 4,104,234 + BT3210 0.48 -0.8 4,104,285 + BT3210 0.54 -0.4 4,104,334 + BT3210 0.60 +1.8 4,104,936 + BT3211 0.85 -2.2 4,104,936 + BT3211 0.85 -1.2 4,104,950 + -2.2 4,105,793 + -0.0 4,105,898 + -0.2 4,105,952 + BT3213 0.17 -2.5
Or see this region's nucleotide sequence