Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2835 and tRNA-Arg are separated by 170 nucleotides tRNA-Arg and BT2836 are separated by 203 nucleotides BT2836 and BT2837 are separated by 3 nucleotides
BT2835: BT2835 - protein-export membrane protein SecD/SecF (NCBI ptt file), at 3,534,369 to 3,537,401
BT2835
BT_t52: tRNA-Arg - tRNA-Arg (RefSeq), at 3,537,572 to 3,537,646
tRNA-Arg
BT2836: BT2836 - 2-oxoglutarate synthase subunit korA (NCBI ptt file), at 3,537,850 to 3,539,700
BT2836
BT2837: BT2837 - 2-oxoglutarate synthase subunit korB (NCBI ptt file), at 3,539,704 to 3,540,714
BT2837
Position (kb)
3537
3538
3539
3540 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3537.382 kb on + strand at 3537.455 kb on + strand at 3537.474 kb on + strand at 3537.482 kb on - strand at 3537.484 kb on - strand at 3537.512 kb on + strand at 3537.519 kb on + strand at 3537.530 kb on - strand at 3537.534 kb on + strand at 3537.535 kb on - strand at 3537.890 kb on + strand at 3537.891 kb on - strand at 3537.893 kb on + strand at 3537.893 kb on + strand at 3537.894 kb on - strand at 3537.921 kb on + strand at 3537.981 kb on + strand at 3537.981 kb on + strand at 3538.075 kb on + strand, within BT2836 at 3538.100 kb on + strand at 3538.101 kb on - strand, within BT2836 at 3538.101 kb on - strand, within BT2836 at 3538.104 kb on - strand, within BT2836 at 3538.164 kb on + strand, within BT2836 at 3538.165 kb on - strand, within BT2836 at 3538.218 kb on + strand, within BT2836 at 3538.284 kb on + strand at 3538.284 kb on + strand, within BT2836 at 3538.284 kb on + strand, within BT2836 at 3538.285 kb on - strand, within BT2836 at 3538.324 kb on - strand, within BT2836 at 3538.324 kb on - strand, within BT2836 at 3538.324 kb on - strand, within BT2836 at 3538.347 kb on + strand, within BT2836 at 3538.348 kb on - strand, within BT2836 at 3538.455 kb on + strand, within BT2836 at 3538.455 kb on + strand, within BT2836 at 3538.456 kb on - strand, within BT2836 at 3538.458 kb on + strand, within BT2836 at 3538.478 kb on - strand, within BT2836 at 3538.478 kb on - strand, within BT2836 at 3538.483 kb on + strand, within BT2836 at 3538.483 kb on + strand, within BT2836 at 3538.484 kb on - strand, within BT2836 at 3538.512 kb on + strand at 3538.513 kb on - strand, within BT2836 at 3538.516 kb on + strand, within BT2836 at 3538.516 kb on + strand, within BT2836 at 3538.556 kb on - strand, within BT2836 at 3538.557 kb on + strand, within BT2836 at 3538.558 kb on - strand, within BT2836 at 3538.563 kb on + strand, within BT2836 at 3538.563 kb on + strand, within BT2836 at 3538.564 kb on - strand, within BT2836 at 3538.567 kb on - strand, within BT2836 at 3538.630 kb on + strand, within BT2836 at 3538.630 kb on + strand, within BT2836 at 3538.630 kb on + strand, within BT2836 at 3538.690 kb on - strand, within BT2836 at 3538.690 kb on - strand, within BT2836 at 3538.781 kb on + strand, within BT2836 at 3538.871 kb on + strand, within BT2836 at 3539.054 kb on + strand, within BT2836 at 3539.054 kb on + strand, within BT2836 at 3539.119 kb on + strand at 3539.145 kb on - strand, within BT2836 at 3539.145 kb on - strand, within BT2836 at 3539.185 kb on - strand, within BT2836 at 3539.236 kb on - strand, within BT2836 at 3539.265 kb on + strand, within BT2836 at 3539.353 kb on + strand, within BT2836 at 3539.354 kb on - strand, within BT2836 at 3539.354 kb on - strand, within BT2836 at 3539.354 kb on - strand at 3539.396 kb on + strand at 3539.396 kb on + strand, within BT2836 at 3539.397 kb on - strand, within BT2836 at 3539.405 kb on + strand, within BT2836 at 3539.405 kb on + strand, within BT2836 at 3539.422 kb on + strand, within BT2836 at 3539.422 kb on + strand, within BT2836 at 3539.423 kb on - strand, within BT2836 at 3539.443 kb on - strand, within BT2836 at 3539.495 kb on - strand, within BT2836 at 3539.528 kb on + strand at 3539.531 kb on + strand at 3539.531 kb on + strand at 3539.535 kb on + strand at 3539.535 kb on + strand at 3539.535 kb on + strand at 3539.535 kb on + strand at 3539.536 kb on - strand at 3539.540 kb on - strand at 3539.552 kb on + strand at 3539.553 kb on - strand at 3539.553 kb on - strand at 3539.553 kb on - strand at 3539.556 kb on - strand at 3539.595 kb on + strand at 3539.617 kb on - strand at 3539.656 kb on - strand at 3539.669 kb on + strand at 3539.670 kb on - strand at 3539.670 kb on - strand at 3539.670 kb on - strand at 3539.670 kb on - strand at 3539.670 kb on - strand at 3539.698 kb on + strand at 3539.699 kb on - strand at 3539.699 kb on - strand at 3539.717 kb on + strand at 3539.717 kb on + strand at 3539.721 kb on + strand at 3539.721 kb on + strand at 3539.722 kb on - strand at 3539.722 kb on - strand at 3539.736 kb on + strand at 3539.822 kb on + strand, within BT2837 at 3539.822 kb on + strand, within BT2837 at 3539.822 kb on + strand, within BT2837 at 3539.836 kb on - strand, within BT2837 at 3539.878 kb on + strand, within BT2837 at 3539.881 kb on + strand at 3539.882 kb on - strand, within BT2837 at 3539.885 kb on + strand, within BT2837 at 3539.886 kb on - strand, within BT2837 at 3539.889 kb on + strand, within BT2837 at 3539.889 kb on + strand, within BT2837 at 3539.889 kb on + strand, within BT2837 at 3539.889 kb on + strand at 3539.890 kb on - strand, within BT2837 at 3539.890 kb on - strand, within BT2837 at 3539.893 kb on + strand, within BT2837 at 3539.893 kb on + strand, within BT2837 at 3539.968 kb on - strand, within BT2837 at 3540.075 kb on + strand, within BT2837 at 3540.075 kb on + strand, within BT2837 at 3540.076 kb on - strand, within BT2837 at 3540.076 kb on - strand, within BT2837 at 3540.079 kb on + strand, within BT2837 at 3540.101 kb on - strand, within BT2837 at 3540.146 kb on - strand, within BT2837 at 3540.219 kb on + strand, within BT2837 at 3540.220 kb on - strand, within BT2837 at 3540.220 kb on - strand, within BT2837 at 3540.273 kb on + strand, within BT2837 at 3540.526 kb on - strand, within BT2837 at 3540.526 kb on - strand, within BT2837 at 3540.606 kb on + strand, within BT2837 at 3540.606 kb on + strand, within BT2837 at 3540.606 kb on + strand, within BT2837 at 3540.606 kb on + strand, within BT2837 at 3540.607 kb on - strand, within BT2837 at 3540.610 kb on + strand, within BT2837 at 3540.611 kb on - strand, within BT2837 at 3540.611 kb on - strand, within BT2837 at 3540.661 kb on + strand at 3540.696 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 3,537,382 + -0.8 3,537,455 + +0.9 3,537,474 + -2.2 3,537,482 - -1.1 3,537,484 - -0.1 3,537,512 + -2.4 3,537,519 + -1.7 3,537,530 - -0.7 3,537,534 + +0.3 3,537,535 - -0.1 3,537,890 + -0.1 3,537,891 - -3.5 3,537,893 + -0.9 3,537,893 + +0.3 3,537,894 - +0.9 3,537,921 + -2.4 3,537,981 + +0.5 3,537,981 + -1.1 3,538,075 + BT2836 0.12 -2.1 3,538,100 + -2.1 3,538,101 - BT2836 0.14 -1.7 3,538,101 - BT2836 0.14 -1.1 3,538,104 - BT2836 0.14 -1.7 3,538,164 + BT2836 0.17 -2.5 3,538,165 - BT2836 0.17 -0.1 3,538,218 + BT2836 0.20 -0.8 3,538,284 + -0.1 3,538,284 + BT2836 0.23 -1.8 3,538,284 + BT2836 0.23 -1.2 3,538,285 - BT2836 0.24 -1.9 3,538,324 - BT2836 0.26 -1.1 3,538,324 - BT2836 0.26 -2.4 3,538,324 - BT2836 0.26 -1.3 3,538,347 + BT2836 0.27 -1.7 3,538,348 - BT2836 0.27 -2.7 3,538,455 + BT2836 0.33 -2.0 3,538,455 + BT2836 0.33 -2.1 3,538,456 - BT2836 0.33 -1.8 3,538,458 + BT2836 0.33 -1.1 3,538,478 - BT2836 0.34 -0.1 3,538,478 - BT2836 0.34 -2.4 3,538,483 + BT2836 0.34 -2.1 3,538,483 + BT2836 0.34 -0.1 3,538,484 - BT2836 0.34 -0.1 3,538,512 + -0.1 3,538,513 - BT2836 0.36 -1.1 3,538,516 + BT2836 0.36 -2.1 3,538,516 + BT2836 0.36 -1.1 3,538,556 - BT2836 0.38 +1.5 3,538,557 + BT2836 0.38 -2.7 3,538,558 - BT2836 0.38 -1.1 3,538,563 + BT2836 0.39 -2.8 3,538,563 + BT2836 0.39 -1.7 3,538,564 - BT2836 0.39 -2.9 3,538,567 - BT2836 0.39 -3.5 3,538,630 + BT2836 0.42 +0.3 3,538,630 + BT2836 0.42 -2.4 3,538,630 + BT2836 0.42 -3.5 3,538,690 - BT2836 0.45 -1.1 3,538,690 - BT2836 0.45 -1.1 3,538,781 + BT2836 0.50 -2.1 3,538,871 + BT2836 0.55 -2.3 3,539,054 + BT2836 0.65 -2.1 3,539,054 + BT2836 0.65 -3.1 3,539,119 + +0.9 3,539,145 - BT2836 0.70 -3.1 3,539,145 - BT2836 0.70 -1.4 3,539,185 - BT2836 0.72 -1.7 3,539,236 - BT2836 0.75 -0.1 3,539,265 + BT2836 0.76 -0.1 3,539,353 + BT2836 0.81 -0.1 3,539,354 - BT2836 0.81 -2.3 3,539,354 - BT2836 0.81 -3.3 3,539,354 - +0.9 3,539,396 + -0.1 3,539,396 + BT2836 0.84 +1.9 3,539,397 - BT2836 0.84 -2.4 3,539,405 + BT2836 0.84 -1.4 3,539,405 + BT2836 0.84 -2.5 3,539,422 + BT2836 0.85 -2.5 3,539,422 + BT2836 0.85 -0.1 3,539,423 - BT2836 0.85 +0.1 3,539,443 - BT2836 0.86 -0.7 3,539,495 - BT2836 0.89 +0.5 3,539,528 + -2.8 3,539,531 + -1.5 3,539,531 + -1.7 3,539,535 + -4.5 3,539,535 + -2.1 3,539,535 + -1.0 3,539,535 + -0.1 3,539,536 - -2.7 3,539,540 - -2.1 3,539,552 + -1.8 3,539,553 - -1.7 3,539,553 - -0.1 3,539,553 - -1.9 3,539,556 - -3.9 3,539,595 + -3.8 3,539,617 - -0.1 3,539,656 - -2.1 3,539,669 + -1.1 3,539,670 - -2.2 3,539,670 - -1.1 3,539,670 - -2.4 3,539,670 - -2.1 3,539,670 - -1.7 3,539,698 + -2.4 3,539,699 - -1.5 3,539,699 - -0.1 3,539,717 + -1.1 3,539,717 + -2.4 3,539,721 + +1.9 3,539,721 + -0.5 3,539,722 - -3.4 3,539,722 - -2.2 3,539,736 + -0.1 3,539,822 + BT2837 0.12 +1.5 3,539,822 + BT2837 0.12 -1.0 3,539,822 + BT2837 0.12 -1.1 3,539,836 - BT2837 0.13 -2.5 3,539,878 + BT2837 0.17 -0.9 3,539,881 + -0.1 3,539,882 - BT2837 0.18 -0.1 3,539,885 + BT2837 0.18 -0.1 3,539,886 - BT2837 0.18 -2.4 3,539,889 + BT2837 0.18 -3.7 3,539,889 + BT2837 0.18 -3.3 3,539,889 + BT2837 0.18 -1.1 3,539,889 + -0.1 3,539,890 - BT2837 0.18 -3.4 3,539,890 - BT2837 0.18 -2.3 3,539,893 + BT2837 0.19 +0.2 3,539,893 + BT2837 0.19 -3.0 3,539,968 - BT2837 0.26 -3.3 3,540,075 + BT2837 0.37 -2.8 3,540,075 + BT2837 0.37 -0.1 3,540,076 - BT2837 0.37 -0.8 3,540,076 - BT2837 0.37 -1.1 3,540,079 + BT2837 0.37 -2.5 3,540,101 - BT2837 0.39 -1.9 3,540,146 - BT2837 0.44 -2.7 3,540,219 + BT2837 0.51 -3.0 3,540,220 - BT2837 0.51 -1.1 3,540,220 - BT2837 0.51 -1.7 3,540,273 + BT2837 0.56 -1.7 3,540,526 - BT2837 0.81 -4.7 3,540,526 - BT2837 0.81 -2.1 3,540,606 + BT2837 0.89 -0.8 3,540,606 + BT2837 0.89 -3.0 3,540,606 + BT2837 0.89 -0.1 3,540,606 + BT2837 0.89 -2.7 3,540,607 - BT2837 0.89 +0.5 3,540,610 + BT2837 0.90 -1.0 3,540,611 - BT2837 0.90 -3.5 3,540,611 - BT2837 0.90 -0.1 3,540,661 + -3.4 3,540,696 + -1.3
Or see this region's nucleotide sequence