Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2313 and BT2314 are separated by 762 nucleotides BT2314 and BT2315 are separated by 155 nucleotides BT2315 and BT2316 are separated by 113 nucleotides
BT2313: BT2313 - conserved hypothetical protein (NCBI ptt file), at 2,886,967 to 2,887,986
BT2313
BT2314: BT2314 - hypothetical protein (NCBI ptt file), at 2,888,749 to 2,888,982
BT2314
BT2315: BT2315 - hypothetical protein (NCBI ptt file), at 2,889,138 to 2,889,620
BT2315
BT2316: BT2316 - conserved hypothetical protein (NCBI ptt file), at 2,889,734 to 2,892,400
BT2316
Position (kb)
2888
2889 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2887.873 kb on + strand, within BT2313 at 2887.874 kb on - strand, within BT2313 at 2887.875 kb on + strand, within BT2313 at 2887.875 kb on + strand, within BT2313 at 2887.876 kb on - strand, within BT2313 at 2887.884 kb on + strand, within BT2313 at 2888.016 kb on + strand at 2888.019 kb on - strand at 2888.019 kb on - strand at 2888.117 kb on + strand at 2888.132 kb on - strand at 2888.141 kb on + strand at 2888.142 kb on - strand at 2888.265 kb on - strand at 2888.266 kb on + strand at 2888.286 kb on - strand at 2888.323 kb on + strand at 2888.452 kb on - strand at 2888.518 kb on - strand at 2888.528 kb on - strand at 2888.532 kb on + strand at 2888.532 kb on + strand at 2888.532 kb on + strand at 2888.533 kb on - strand at 2888.601 kb on + strand at 2888.789 kb on + strand, within BT2314 at 2888.789 kb on + strand, within BT2314 at 2888.875 kb on - strand, within BT2314 at 2888.908 kb on + strand, within BT2314 at 2888.937 kb on - strand, within BT2314 at 2889.024 kb on - strand at 2889.024 kb on - strand at 2889.059 kb on + strand at 2889.060 kb on - strand at 2889.060 kb on - strand at 2889.080 kb on + strand at 2889.081 kb on - strand at 2889.081 kb on - strand at 2889.093 kb on - strand at 2889.093 kb on - strand at 2889.134 kb on + strand at 2889.134 kb on + strand at 2889.134 kb on + strand at 2889.136 kb on + strand at 2889.169 kb on - strand at 2889.173 kb on + strand at 2889.174 kb on - strand at 2889.174 kb on - strand at 2889.286 kb on - strand, within BT2315 at 2889.405 kb on + strand, within BT2315 at 2889.406 kb on - strand, within BT2315 at 2889.425 kb on + strand, within BT2315 at 2889.426 kb on - strand, within BT2315 at 2889.426 kb on - strand, within BT2315 at 2889.448 kb on - strand, within BT2315 at 2889.471 kb on + strand, within BT2315 at 2889.510 kb on - strand, within BT2315 at 2889.573 kb on - strand at 2889.638 kb on - strand at 2889.670 kb on - strand at 2889.781 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 2,887,873 + BT2313 0.89 +0.0 2,887,874 - BT2313 0.89 -0.8 2,887,875 + BT2313 0.89 +0.3 2,887,875 + BT2313 0.89 -1.8 2,887,876 - BT2313 0.89 +3.9 2,887,884 + BT2313 0.90 -0.3 2,888,016 + -0.7 2,888,019 - +0.0 2,888,019 - +0.4 2,888,117 + -1.4 2,888,132 - -0.1 2,888,141 + -0.9 2,888,142 - -0.3 2,888,265 - -1.6 2,888,266 + -1.4 2,888,286 - -1.3 2,888,323 + -1.6 2,888,452 - -1.4 2,888,518 - -0.6 2,888,528 - -1.0 2,888,532 + -1.0 2,888,532 + -1.9 2,888,532 + -1.6 2,888,533 - -1.0 2,888,601 + -1.0 2,888,789 + BT2314 0.17 +0.4 2,888,789 + BT2314 0.17 +1.4 2,888,875 - BT2314 0.54 -2.5 2,888,908 + BT2314 0.68 -3.1 2,888,937 - BT2314 0.80 -2.3 2,889,024 - -2.0 2,889,024 - -2.7 2,889,059 + +0.0 2,889,060 - +1.1 2,889,060 - -1.1 2,889,080 + -2.0 2,889,081 - -2.4 2,889,081 - -2.0 2,889,093 - -1.4 2,889,093 - -2.8 2,889,134 + +1.6 2,889,134 + -0.4 2,889,134 + -1.3 2,889,136 + -0.6 2,889,169 - +1.0 2,889,173 + +1.4 2,889,174 - -2.0 2,889,174 - -0.7 2,889,286 - BT2315 0.31 -0.4 2,889,405 + BT2315 0.55 -3.2 2,889,406 - BT2315 0.55 +0.0 2,889,425 + BT2315 0.59 -1.0 2,889,426 - BT2315 0.60 +3.7 2,889,426 - BT2315 0.60 +0.6 2,889,448 - BT2315 0.64 -2.6 2,889,471 + BT2315 0.69 -0.4 2,889,510 - BT2315 0.77 -0.5 2,889,573 - -1.0 2,889,638 - -1.6 2,889,670 - +0.0 2,889,781 - +0.4
Or see this region's nucleotide sequence