Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1953 and BT1954 are separated by 18 nucleotides BT1954 and BT1955 are separated by 28 nucleotides
BT1953: BT1953 - putative TonB-linked outer membrane receptor (NCBI ptt file), at 2,451,964 to 2,454,042
BT1953
BT1954: BT1954 - putative surface layer protein (NCBI ptt file), at 2,454,061 to 2,455,161
BT1954
BT1955: BT1955 - putative cell wall biogenesis protein (NCBI ptt file), at 2,455,190 to 2,457,280
BT1955
Position (kb)
2454
2455
2456 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2453.081 kb on + strand, within BT1953 at 2453.082 kb on - strand, within BT1953 at 2453.098 kb on + strand, within BT1953 at 2453.098 kb on + strand, within BT1953 at 2453.098 kb on + strand, within BT1953 at 2453.099 kb on - strand, within BT1953 at 2453.101 kb on - strand, within BT1953 at 2453.102 kb on + strand, within BT1953 at 2453.250 kb on + strand, within BT1953 at 2453.251 kb on - strand, within BT1953 at 2453.328 kb on - strand, within BT1953 at 2453.390 kb on + strand, within BT1953 at 2453.540 kb on + strand, within BT1953 at 2453.838 kb on + strand at 2453.896 kb on + strand at 2453.896 kb on + strand at 2453.897 kb on - strand at 2453.897 kb on - strand at 2453.907 kb on + strand at 2453.908 kb on - strand at 2453.908 kb on - strand at 2453.908 kb on - strand at 2453.908 kb on - strand at 2453.928 kb on + strand at 2453.944 kb on + strand at 2453.945 kb on - strand at 2453.958 kb on + strand at 2454.028 kb on - strand at 2454.180 kb on - strand, within BT1954 at 2454.288 kb on + strand, within BT1954 at 2454.289 kb on - strand, within BT1954 at 2454.502 kb on - strand, within BT1954 at 2454.508 kb on + strand, within BT1954 at 2454.509 kb on - strand, within BT1954 at 2454.575 kb on + strand, within BT1954 at 2454.705 kb on + strand, within BT1954 at 2454.706 kb on - strand, within BT1954 at 2454.732 kb on - strand, within BT1954 at 2454.761 kb on + strand, within BT1954 at 2454.807 kb on + strand, within BT1954 at 2454.860 kb on - strand, within BT1954 at 2454.861 kb on + strand, within BT1954 at 2454.861 kb on + strand, within BT1954 at 2454.920 kb on + strand, within BT1954 at 2455.204 kb on + strand at 2455.205 kb on - strand at 2455.243 kb on - strand at 2455.423 kb on + strand, within BT1955 at 2455.430 kb on + strand, within BT1955 at 2455.433 kb on - strand, within BT1955 at 2455.525 kb on + strand, within BT1955 at 2455.564 kb on + strand, within BT1955 at 2455.565 kb on - strand, within BT1955 at 2455.569 kb on + strand, within BT1955 at 2455.569 kb on + strand, within BT1955 at 2455.572 kb on - strand, within BT1955 at 2455.604 kb on + strand, within BT1955 at 2455.642 kb on - strand, within BT1955 at 2455.705 kb on + strand, within BT1955 at 2455.707 kb on + strand, within BT1955 at 2455.707 kb on + strand, within BT1955 at 2455.708 kb on - strand, within BT1955 at 2455.708 kb on - strand, within BT1955 at 2455.714 kb on - strand, within BT1955 at 2455.722 kb on - strand, within BT1955 at 2455.785 kb on + strand, within BT1955 at 2455.785 kb on + strand, within BT1955 at 2455.787 kb on + strand at 2455.788 kb on - strand, within BT1955 at 2455.812 kb on + strand, within BT1955 at 2455.812 kb on + strand, within BT1955 at 2455.838 kb on - strand, within BT1955 at 2455.882 kb on + strand, within BT1955 at 2455.883 kb on - strand, within BT1955 at 2456.049 kb on + strand, within BT1955 at 2456.050 kb on - strand, within BT1955 at 2456.129 kb on + strand, within BT1955 at 2456.141 kb on + strand, within BT1955
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 2,453,081 + BT1953 0.54 -1.9 2,453,082 - BT1953 0.54 -4.0 2,453,098 + BT1953 0.55 -1.8 2,453,098 + BT1953 0.55 +1.1 2,453,098 + BT1953 0.55 -1.9 2,453,099 - BT1953 0.55 -0.9 2,453,101 - BT1953 0.55 -3.1 2,453,102 + BT1953 0.55 -5.1 2,453,250 + BT1953 0.62 -1.5 2,453,251 - BT1953 0.62 -1.9 2,453,328 - BT1953 0.66 -3.4 2,453,390 + BT1953 0.69 -2.6 2,453,540 + BT1953 0.76 -0.9 2,453,838 + +0.5 2,453,896 + -0.9 2,453,896 + -2.5 2,453,897 - +0.1 2,453,897 - -4.1 2,453,907 + -2.1 2,453,908 - -3.1 2,453,908 - -0.9 2,453,908 - -1.9 2,453,908 - -2.5 2,453,928 + -1.5 2,453,944 + -1.9 2,453,945 - +0.1 2,453,958 + -0.9 2,454,028 - -3.3 2,454,180 - BT1954 0.11 -1.3 2,454,288 + BT1954 0.21 -1.7 2,454,289 - BT1954 0.21 -2.9 2,454,502 - BT1954 0.40 -1.5 2,454,508 + BT1954 0.41 +0.1 2,454,509 - BT1954 0.41 +0.1 2,454,575 + BT1954 0.47 -4.0 2,454,705 + BT1954 0.58 -1.5 2,454,706 - BT1954 0.59 -0.3 2,454,732 - BT1954 0.61 -2.3 2,454,761 + BT1954 0.64 -0.9 2,454,807 + BT1954 0.68 -2.7 2,454,860 - BT1954 0.73 -2.5 2,454,861 + BT1954 0.73 -2.1 2,454,861 + BT1954 0.73 -2.0 2,454,920 + BT1954 0.78 -2.3 2,455,204 + -2.3 2,455,205 - +1.1 2,455,243 - -0.9 2,455,423 + BT1955 0.11 -1.9 2,455,430 + BT1955 0.11 -4.3 2,455,433 - BT1955 0.12 -1.0 2,455,525 + BT1955 0.16 +0.1 2,455,564 + BT1955 0.18 +0.1 2,455,565 - BT1955 0.18 +0.1 2,455,569 + BT1955 0.18 -1.9 2,455,569 + BT1955 0.18 -0.9 2,455,572 - BT1955 0.18 +0.1 2,455,604 + BT1955 0.20 -0.9 2,455,642 - BT1955 0.22 -1.3 2,455,705 + BT1955 0.25 -1.8 2,455,707 + BT1955 0.25 +0.6 2,455,707 + BT1955 0.25 +0.1 2,455,708 - BT1955 0.25 -1.9 2,455,708 - BT1955 0.25 -4.3 2,455,714 - BT1955 0.25 -1.2 2,455,722 - BT1955 0.25 +0.1 2,455,785 + BT1955 0.28 +0.1 2,455,785 + BT1955 0.28 -0.9 2,455,787 + -0.9 2,455,788 - BT1955 0.29 -0.9 2,455,812 + BT1955 0.30 -0.9 2,455,812 + BT1955 0.30 +0.1 2,455,838 - BT1955 0.31 +0.1 2,455,882 + BT1955 0.33 -4.0 2,455,883 - BT1955 0.33 -3.1 2,456,049 + BT1955 0.41 -2.9 2,456,050 - BT1955 0.41 -0.2 2,456,129 + BT1955 0.45 -1.5 2,456,141 + BT1955 0.45 -2.7
Or see this region's nucleotide sequence