Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1410 and BT1411 are separated by 213 nucleotides BT1411 and BT1412 are separated by 195 nucleotides
BT1410: BT1410 - putative metallo-beta-lactamase (NCBI ptt file), at 1,743,635 to 1,744,456
BT1410
BT1411: BT1411 - methylated-DNA--protein-cysteine methyltransferase (NCBI ptt file), at 1,744,670 to 1,745,215
BT1411
BT1412: BT1412 - iron-sulfur flavoprotein (NCBI ptt file), at 1,745,411 to 1,745,950
BT1412
Position (kb)
1744
1745
1746 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1743.717 kb on + strand at 1743.721 kb on + strand, within BT1410 at 1743.814 kb on + strand, within BT1410 at 1743.821 kb on - strand, within BT1410 at 1743.872 kb on + strand, within BT1410 at 1743.884 kb on + strand, within BT1410 at 1744.042 kb on + strand, within BT1410 at 1744.042 kb on + strand, within BT1410 at 1744.134 kb on + strand, within BT1410 at 1744.134 kb on + strand, within BT1410 at 1744.265 kb on + strand, within BT1410 at 1744.271 kb on + strand, within BT1410 at 1744.272 kb on - strand, within BT1410 at 1744.375 kb on + strand at 1744.416 kb on + strand at 1744.448 kb on - strand at 1744.471 kb on + strand at 1744.564 kb on + strand at 1744.625 kb on - strand at 1744.634 kb on + strand at 1744.643 kb on - strand at 1744.691 kb on - strand at 1744.699 kb on + strand at 1744.700 kb on - strand at 1744.984 kb on - strand, within BT1411 at 1745.015 kb on + strand, within BT1411 at 1745.015 kb on + strand, within BT1411 at 1745.134 kb on + strand, within BT1411 at 1745.147 kb on + strand, within BT1411 at 1745.168 kb on - strand at 1745.282 kb on + strand at 1745.282 kb on + strand at 1745.285 kb on - strand at 1745.298 kb on + strand at 1745.321 kb on - strand at 1745.338 kb on - strand at 1745.339 kb on + strand at 1745.340 kb on - strand at 1745.342 kb on - strand at 1745.351 kb on - strand at 1745.377 kb on + strand at 1745.378 kb on - strand at 1745.395 kb on + strand at 1745.433 kb on + strand at 1745.463 kb on - strand at 1745.486 kb on + strand, within BT1412 at 1745.656 kb on + strand, within BT1412 at 1745.683 kb on - strand, within BT1412 at 1745.779 kb on - strand, within BT1412 at 1745.794 kb on + strand, within BT1412 at 1745.795 kb on - strand, within BT1412 at 1745.893 kb on + strand, within BT1412 at 1746.024 kb on + strand at 1746.060 kb on - strand at 1746.177 kb on + strand at 1746.178 kb on - strand at 1746.185 kb on - strand at 1746.200 kb on - strand at 1746.210 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 1,743,717 + -0.9 1,743,721 + BT1410 0.10 +0.1 1,743,814 + BT1410 0.22 -0.9 1,743,821 - BT1410 0.23 +2.1 1,743,872 + BT1410 0.29 +0.1 1,743,884 + BT1410 0.30 -0.4 1,744,042 + BT1410 0.50 +1.4 1,744,042 + BT1410 0.50 -0.9 1,744,134 + BT1410 0.61 +2.1 1,744,134 + BT1410 0.61 +0.4 1,744,265 + BT1410 0.77 -2.2 1,744,271 + BT1410 0.77 -1.9 1,744,272 - BT1410 0.77 +0.1 1,744,375 + -1.6 1,744,416 + +1.2 1,744,448 - -0.9 1,744,471 + -0.9 1,744,564 + -2.7 1,744,625 - +1.6 1,744,634 + +0.1 1,744,643 - -0.8 1,744,691 - +0.3 1,744,699 + -3.1 1,744,700 - +1.6 1,744,984 - BT1411 0.58 -0.9 1,745,015 + BT1411 0.63 -1.9 1,745,015 + BT1411 0.63 +0.1 1,745,134 + BT1411 0.85 -2.3 1,745,147 + BT1411 0.87 +0.1 1,745,168 - -1.5 1,745,282 + -1.0 1,745,282 + +0.1 1,745,285 - +0.1 1,745,298 + +0.9 1,745,321 - -0.9 1,745,338 - -3.1 1,745,339 + -0.9 1,745,340 - -0.9 1,745,342 - +0.6 1,745,351 - +0.5 1,745,377 + -0.9 1,745,378 - -2.5 1,745,395 + -0.7 1,745,433 + -0.8 1,745,463 - -0.9 1,745,486 + BT1412 0.14 -2.3 1,745,656 + BT1412 0.45 -0.5 1,745,683 - BT1412 0.50 -0.3 1,745,779 - BT1412 0.68 -0.8 1,745,794 + BT1412 0.71 +0.6 1,745,795 - BT1412 0.71 +0.1 1,745,893 + BT1412 0.89 +1.6 1,746,024 + +0.1 1,746,060 - +0.1 1,746,177 + +2.1 1,746,178 - +1.5 1,746,185 - -1.5 1,746,200 - -2.5 1,746,210 + -2.5
Or see this region's nucleotide sequence