Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1377 and BT1378 are separated by 62 nucleotides BT1378 and BT1379 are separated by 108 nucleotides BT1379 and BT1380 are separated by 171 nucleotides BT1380 and BT1381 overlap by 1 nucleotides
BT1377: BT1377 - phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphohydrolase (NCBI ptt file), at 1,705,493 to 1,706,104
BT1377
BT1378: BT1378 - imidazole glycerol phosphate synthase subunit hisF (NCBI ptt file), at 1,706,167 to 1,706,922
BT1378
BT1379: BT1379 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 1,707,031 to 1,707,750
BT1379
BT1380: BT1380 - imidazole glycerol phosphate synthase subunit hisH (NCBI ptt file), at 1,707,922 to 1,708,512
BT1380
BT1381: BT1381 - formyltetrahydrofolate deformylase (NCBI ptt file), at 1,708,512 to 1,709,366
BT1381
Position (kb)
1707
1708 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1706.113 kb on - strand at 1706.123 kb on + strand at 1706.124 kb on - strand at 1706.140 kb on - strand at 1706.276 kb on - strand, within BT1378 at 1706.451 kb on + strand, within BT1378 at 1706.452 kb on - strand, within BT1378 at 1706.578 kb on - strand, within BT1378 at 1706.692 kb on - strand, within BT1378 at 1706.700 kb on + strand, within BT1378 at 1706.706 kb on + strand, within BT1378 at 1706.707 kb on - strand, within BT1378 at 1706.725 kb on + strand, within BT1378 at 1706.888 kb on - strand at 1706.934 kb on - strand at 1706.953 kb on - strand at 1706.959 kb on + strand at 1707.019 kb on + strand at 1707.028 kb on + strand at 1707.033 kb on - strand at 1707.041 kb on - strand at 1707.061 kb on + strand at 1707.061 kb on + strand at 1707.063 kb on + strand at 1707.063 kb on + strand at 1707.063 kb on + strand at 1707.211 kb on - strand, within BT1379 at 1707.240 kb on + strand, within BT1379 at 1707.240 kb on + strand, within BT1379 at 1707.273 kb on + strand, within BT1379 at 1707.273 kb on + strand, within BT1379 at 1707.273 kb on + strand, within BT1379 at 1707.274 kb on - strand, within BT1379 at 1707.274 kb on - strand, within BT1379 at 1707.274 kb on - strand, within BT1379 at 1707.278 kb on - strand, within BT1379 at 1707.345 kb on + strand, within BT1379 at 1707.345 kb on + strand, within BT1379 at 1707.345 kb on + strand, within BT1379 at 1707.346 kb on - strand, within BT1379 at 1707.346 kb on - strand, within BT1379 at 1707.392 kb on - strand, within BT1379 at 1707.665 kb on + strand, within BT1379 at 1707.666 kb on - strand, within BT1379 at 1707.666 kb on - strand, within BT1379 at 1707.678 kb on + strand, within BT1379 at 1707.703 kb on + strand at 1707.720 kb on + strand at 1707.721 kb on - strand at 1707.756 kb on + strand at 1707.759 kb on - strand at 1707.760 kb on + strand at 1707.814 kb on - strand at 1707.857 kb on - strand at 1707.887 kb on - strand at 1707.899 kb on - strand at 1707.903 kb on + strand at 1708.009 kb on - strand, within BT1380 at 1708.035 kb on + strand, within BT1380 at 1708.076 kb on + strand, within BT1380 at 1708.461 kb on - strand at 1708.538 kb on + strand at 1708.538 kb on + strand at 1708.614 kb on - strand, within BT1381 at 1708.646 kb on + strand, within BT1381 at 1708.656 kb on + strand, within BT1381 at 1708.702 kb on + strand, within BT1381 at 1708.702 kb on + strand, within BT1381
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 1,706,113 - -1.8 1,706,123 + -2.5 1,706,124 - -1.5 1,706,140 - -1.9 1,706,276 - BT1378 0.14 -3.1 1,706,451 + BT1378 0.38 +0.1 1,706,452 - BT1378 0.38 -0.9 1,706,578 - BT1378 0.54 -1.5 1,706,692 - BT1378 0.69 -2.8 1,706,700 + BT1378 0.71 -0.9 1,706,706 + BT1378 0.71 -0.9 1,706,707 - BT1378 0.71 -1.5 1,706,725 + BT1378 0.74 +0.1 1,706,888 - -4.6 1,706,934 - -1.2 1,706,953 - -2.2 1,706,959 + -1.5 1,707,019 + -0.9 1,707,028 + -2.5 1,707,033 - +0.1 1,707,041 - -1.5 1,707,061 + +1.3 1,707,061 + -0.9 1,707,063 + -3.1 1,707,063 + +0.1 1,707,063 + -3.2 1,707,211 - BT1379 0.25 +0.1 1,707,240 + BT1379 0.29 +0.1 1,707,240 + BT1379 0.29 -3.6 1,707,273 + BT1379 0.34 -3.9 1,707,273 + BT1379 0.34 -1.5 1,707,273 + BT1379 0.34 -3.5 1,707,274 - BT1379 0.34 -4.9 1,707,274 - BT1379 0.34 -3.9 1,707,274 - BT1379 0.34 -2.5 1,707,278 - BT1379 0.34 +0.1 1,707,345 + BT1379 0.44 -2.8 1,707,345 + BT1379 0.44 -3.5 1,707,345 + BT1379 0.44 -3.1 1,707,346 - BT1379 0.44 +0.1 1,707,346 - BT1379 0.44 -1.9 1,707,392 - BT1379 0.50 -0.9 1,707,665 + BT1379 0.88 -2.4 1,707,666 - BT1379 0.88 +0.1 1,707,666 - BT1379 0.88 -4.6 1,707,678 + BT1379 0.90 -1.9 1,707,703 + +0.1 1,707,720 + -4.3 1,707,721 - +0.1 1,707,756 + +0.1 1,707,759 - -0.9 1,707,760 + -1.5 1,707,814 - -0.9 1,707,857 - -0.3 1,707,887 - +0.1 1,707,899 - -1.1 1,707,903 + -4.5 1,708,009 - BT1380 0.15 -0.2 1,708,035 + BT1380 0.19 -0.9 1,708,076 + BT1380 0.26 +0.1 1,708,461 - -3.6 1,708,538 + -4.3 1,708,538 + -1.5 1,708,614 - BT1381 0.12 -0.9 1,708,646 + BT1381 0.16 -3.1 1,708,656 + BT1381 0.17 -2.6 1,708,702 + BT1381 0.22 -1.5 1,708,702 + BT1381 0.22 +0.1
Or see this region's nucleotide sequence