Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1254 and BT1255 are separated by 124 nucleotides BT1255 and BT1256 overlap by 1 nucleotides BT1256 and BT1257 are separated by 204 nucleotides
BT1254: BT1254 - inner membrane protein (NCBI ptt file), at 1,559,674 to 1,561,029
BT1254
BT1255: BT1255 - hypothetical protein (NCBI ptt file), at 1,561,154 to 1,561,738
BT1255
BT1256: BT1256 - conserved hypothetical protein (NCBI ptt file), at 1,561,738 to 1,562,019
BT1256
BT1257: BT1257 - conserved hypothetical protein (NCBI ptt file), at 1,562,224 to 1,562,691
BT1257
Position (kb)
1561
1562
1563 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1560.748 kb on + strand, within BT1254 at 1560.748 kb on + strand, within BT1254 at 1560.819 kb on + strand, within BT1254 at 1560.825 kb on - strand, within BT1254 at 1560.840 kb on + strand, within BT1254 at 1560.840 kb on + strand, within BT1254 at 1560.894 kb on - strand at 1560.899 kb on + strand at 1560.910 kb on + strand at 1560.911 kb on - strand at 1560.989 kb on + strand at 1560.994 kb on + strand at 1560.995 kb on - strand at 1561.038 kb on - strand at 1561.044 kb on + strand at 1561.045 kb on - strand at 1561.051 kb on + strand at 1561.097 kb on + strand at 1561.110 kb on + strand at 1561.132 kb on + strand at 1561.170 kb on - strand at 1561.369 kb on + strand, within BT1255 at 1561.369 kb on + strand, within BT1255 at 1561.370 kb on - strand, within BT1255 at 1561.467 kb on - strand, within BT1255 at 1561.473 kb on - strand, within BT1255 at 1561.615 kb on - strand, within BT1255 at 1561.641 kb on - strand, within BT1255 at 1561.641 kb on - strand, within BT1255 at 1561.641 kb on - strand, within BT1255 at 1561.652 kb on + strand, within BT1255 at 1561.653 kb on - strand, within BT1255 at 1561.799 kb on + strand, within BT1256 at 1561.800 kb on - strand, within BT1256 at 1562.020 kb on - strand at 1562.178 kb on + strand at 1562.179 kb on - strand at 1562.231 kb on + strand at 1562.236 kb on + strand at 1562.238 kb on + strand at 1562.240 kb on + strand at 1562.287 kb on - strand, within BT1257 at 1562.292 kb on + strand, within BT1257 at 1562.330 kb on + strand, within BT1257 at 1562.383 kb on + strand, within BT1257 at 1562.446 kb on + strand, within BT1257 at 1562.479 kb on + strand, within BT1257 at 1562.482 kb on - strand, within BT1257 at 1562.577 kb on - strand, within BT1257 at 1562.580 kb on + strand, within BT1257 at 1562.580 kb on + strand, within BT1257 at 1562.581 kb on - strand, within BT1257 at 1562.581 kb on - strand, within BT1257 at 1562.639 kb on + strand, within BT1257 at 1562.653 kb on - strand at 1562.731 kb on - strand at 1562.763 kb on + strand at 1562.820 kb on - strand at 1562.847 kb on - strand at 1562.875 kb on + strand at 1562.888 kb on - strand at 1562.984 kb on + strand at 1562.985 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 1,560,748 + BT1254 0.79 -0.3 1,560,748 + BT1254 0.79 +0.0 1,560,819 + BT1254 0.84 +0.0 1,560,825 - BT1254 0.85 +0.6 1,560,840 + BT1254 0.86 -1.0 1,560,840 + BT1254 0.86 +0.4 1,560,894 - +0.8 1,560,899 + -1.2 1,560,910 + -1.4 1,560,911 - -0.7 1,560,989 + -1.5 1,560,994 + -1.0 1,560,995 - -2.8 1,561,038 - -1.6 1,561,044 + -1.0 1,561,045 - -1.0 1,561,051 + -2.0 1,561,097 + -2.0 1,561,110 + +1.6 1,561,132 + -1.0 1,561,170 - -0.2 1,561,369 + BT1255 0.37 -3.9 1,561,369 + BT1255 0.37 -1.6 1,561,370 - BT1255 0.37 +0.0 1,561,467 - BT1255 0.54 -0.2 1,561,473 - BT1255 0.55 -0.2 1,561,615 - BT1255 0.79 -1.0 1,561,641 - BT1255 0.83 +0.0 1,561,641 - BT1255 0.83 +0.2 1,561,641 - BT1255 0.83 +0.0 1,561,652 + BT1255 0.85 -1.0 1,561,653 - BT1255 0.85 -1.1 1,561,799 + BT1256 0.22 -1.0 1,561,800 - BT1256 0.22 -2.8 1,562,020 - -1.6 1,562,178 + +0.1 1,562,179 - -3.0 1,562,231 + +0.3 1,562,236 + -0.7 1,562,238 + -0.6 1,562,240 + -0.4 1,562,287 - BT1257 0.13 +0.0 1,562,292 + BT1257 0.15 +0.0 1,562,330 + BT1257 0.23 +0.1 1,562,383 + BT1257 0.34 +0.5 1,562,446 + BT1257 0.47 -1.0 1,562,479 + BT1257 0.54 +0.0 1,562,482 - BT1257 0.55 +0.0 1,562,577 - BT1257 0.75 +0.0 1,562,580 + BT1257 0.76 -3.1 1,562,580 + BT1257 0.76 -0.1 1,562,581 - BT1257 0.76 +0.6 1,562,581 - BT1257 0.76 -1.0 1,562,639 + BT1257 0.89 -0.3 1,562,653 - -2.3 1,562,731 - -0.4 1,562,763 + +0.0 1,562,820 - -1.6 1,562,847 - -1.3 1,562,875 + -1.6 1,562,888 - +0.0 1,562,984 + -2.0 1,562,985 - +2.0
Or see this region's nucleotide sequence