Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0890 and BT0891 are separated by 75 nucleotides BT0891 and BT0892 overlap by 13 nucleotides BT0892 and BT0893 are separated by 218 nucleotides BT0893 and BT0894 are separated by 76 nucleotides
BT0890: BT0890 - hypothetical protein (NCBI ptt file), at 1,093,156 to 1,093,626
BT0890
BT0891: BT0891 - dihydroorotate dehydrogenase electron transfer subunit (NCBI ptt file), at 1,093,702 to 1,094,478
BT0891
BT0892: BT0892 - dihydroorotate dehydrogenase (NCBI ptt file), at 1,094,466 to 1,095,377
BT0892
BT0893: BT0893 - tRNA (Guanine-N(1)-methyltransferase (NCBI ptt file), at 1,095,596 to 1,096,273
BT0893
BT0894: BT0894 - DNA ligase (NCBI ptt file), at 1,096,350 to 1,098,350
BT0894
Position (kb)
1094
1095
1096 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1093.600 kb on + strand at 1093.699 kb on + strand at 1094.002 kb on + strand, within BT0891 at 1094.004 kb on + strand, within BT0891 at 1094.072 kb on + strand, within BT0891 at 1094.185 kb on + strand, within BT0891 at 1094.188 kb on - strand, within BT0891 at 1094.241 kb on + strand, within BT0891 at 1094.358 kb on + strand, within BT0891 at 1094.358 kb on + strand, within BT0891 at 1094.669 kb on - strand, within BT0892 at 1094.732 kb on - strand, within BT0892 at 1094.837 kb on - strand at 1094.927 kb on - strand, within BT0892 at 1095.470 kb on + strand at 1095.716 kb on - strand, within BT0893 at 1096.314 kb on + strand at 1096.333 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 1,093,600 + -2.0 1,093,699 + -3.1 1,094,002 + BT0891 0.39 -2.0 1,094,004 + BT0891 0.39 -2.5 1,094,072 + BT0891 0.48 -1.5 1,094,185 + BT0891 0.62 +0.0 1,094,188 - BT0891 0.63 -1.5 1,094,241 + BT0891 0.69 -2.8 1,094,358 + BT0891 0.84 +1.4 1,094,358 + BT0891 0.84 -2.0 1,094,669 - BT0892 0.22 -0.5 1,094,732 - BT0892 0.29 -2.0 1,094,837 - -1.5 1,094,927 - BT0892 0.51 -2.3 1,095,470 + -1.5 1,095,716 - BT0893 0.18 -2.3 1,096,314 + -2.3 1,096,333 + +0.0
Or see this region's nucleotide sequence