Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0650 and BT0651 are separated by 80 nucleotides BT0651 and BT0652 are separated by 12 nucleotides BT0652 and BT0653 are separated by 4 nucleotides
BT0650: BT0650 - thiamine biosynthesis protein ThiC (NCBI ptt file), at 800,221 to 801,918
BT0650
BT0651: BT0651 - thiamine biosynthesis protein ThiG (NCBI ptt file), at 801,999 to 802,772
BT0651
BT0652: BT0652 - putative thiamine-phosphate pyrophosphorylase (NCBI ptt file), at 802,785 to 803,414
BT0652
BT0653: BT0653 - thiS protein, involved in thiamine biosynthesis (NCBI ptt file), at 803,419 to 803,619
BT0653
Position (kb) 801
802
803 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 801.009 kb on + strand, within BT0650 at 801.009 kb on + strand, within BT0650 at 801.010 kb on - strand, within BT0650 at 801.010 kb on - strand, within BT0650 at 801.137 kb on + strand, within BT0650 at 801.201 kb on + strand, within BT0650 at 801.201 kb on + strand, within BT0650 at 801.432 kb on + strand, within BT0650 at 801.711 kb on + strand, within BT0650 at 801.758 kb on + strand at 801.760 kb on + strand at 801.760 kb on + strand at 801.765 kb on - strand at 801.972 kb on + strand at 801.976 kb on - strand at 801.983 kb on - strand at 801.986 kb on + strand at 802.159 kb on - strand, within BT0651 at 802.295 kb on + strand, within BT0651 at 802.496 kb on + strand, within BT0651 at 802.592 kb on + strand, within BT0651 at 802.596 kb on - strand at 802.692 kb on + strand, within BT0651 at 802.693 kb on - strand, within BT0651 at 802.905 kb on - strand, within BT0652 at 802.905 kb on - strand, within BT0652 at 802.983 kb on - strand, within BT0652 at 803.057 kb on - strand, within BT0652 at 803.372 kb on + strand at 803.396 kb on + strand at 803.667 kb on + strand at 803.668 kb on - strand at 803.682 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 801,009 + BT0650 0.46 -0.7 801,009 + BT0650 0.46 -0.9 801,010 - BT0650 0.46 +0.3 801,010 - BT0650 0.46 -1.6 801,137 + BT0650 0.54 -0.3 801,201 + BT0650 0.58 -1.1 801,201 + BT0650 0.58 -1.5 801,432 + BT0650 0.71 -2.3 801,711 + BT0650 0.88 -0.0 801,758 + -0.0 801,760 + -0.7 801,760 + -0.0 801,765 - -0.0 801,972 + -0.0 801,976 - -1.0 801,983 - -0.0 801,986 + -2.0 802,159 - BT0651 0.21 -0.0 802,295 + BT0651 0.38 -2.9 802,496 + BT0651 0.64 -0.3 802,592 + BT0651 0.77 -1.8 802,596 - +1.0 802,692 + BT0651 0.90 -2.0 802,693 - BT0651 0.90 -1.0 802,905 - BT0652 0.19 -3.8 802,905 - BT0652 0.19 +0.6 802,983 - BT0652 0.31 -0.0 803,057 - BT0652 0.43 -2.0 803,372 + -0.1 803,396 + +1.0 803,667 + -0.0 803,668 - -1.6 803,682 + -0.7
Or see this region's nucleotide sequence