Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0527 and BT0528 are separated by 18 nucleotides BT0528 and BT0529 are separated by 30 nucleotides BT0529 and BT0530 are separated by 78 nucleotides
BT0527: BT0527 - tryptophan synthase alpha chain (NCBI ptt file), at 651,009 to 651,779
BT0527
BT0528: BT0528 - phosphoribosylanthranilate isomerase (EC 5.3.1.24) (from data) , at 651,798 to 652,421
BT0528
BT0529: BT0529 - indole-3-glycerol phosphate synthase (NCBI ptt file), at 652,452 to 653,234
BT0529
BT0530: BT0530 - anthranilate phosphoribosyltransferase (EC 2.4.2.18) (from data) , at 653,313 to 654,311
BT0530
Position (kb)
652
653
654 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 651.473 kb on + strand, within BT0527 at 651.474 kb on - strand, within BT0527 at 651.477 kb on - strand, within BT0527 at 651.484 kb on - strand, within BT0527 at 651.484 kb on - strand, within BT0527 at 651.498 kb on + strand, within BT0527 at 651.514 kb on - strand, within BT0527 at 651.514 kb on - strand, within BT0527 at 651.574 kb on - strand, within BT0527 at 651.674 kb on + strand, within BT0527 at 651.977 kb on - strand, within BT0528 at 652.021 kb on + strand, within BT0528 at 652.077 kb on + strand, within BT0528 at 652.156 kb on + strand, within BT0528 at 652.189 kb on + strand, within BT0528 at 652.190 kb on - strand, within BT0528 at 652.190 kb on - strand, within BT0528 at 652.350 kb on + strand, within BT0528 at 652.351 kb on - strand, within BT0528 at 652.395 kb on + strand at 652.448 kb on - strand at 652.449 kb on + strand at 652.453 kb on + strand at 652.454 kb on - strand at 652.645 kb on - strand, within BT0529 at 652.709 kb on + strand, within BT0529 at 652.710 kb on - strand, within BT0529 at 652.710 kb on - strand, within BT0529 at 652.717 kb on - strand, within BT0529 at 652.857 kb on + strand, within BT0529 at 652.865 kb on - strand, within BT0529 at 652.890 kb on + strand, within BT0529 at 653.000 kb on - strand, within BT0529 at 653.000 kb on - strand, within BT0529 at 653.078 kb on + strand, within BT0529 at 653.114 kb on + strand, within BT0529 at 653.142 kb on - strand at 653.169 kb on - strand at 653.205 kb on - strand at 653.224 kb on - strand at 653.304 kb on + strand at 653.305 kb on - strand at 653.307 kb on + strand at 653.312 kb on - strand at 653.424 kb on + strand, within BT0530 at 653.451 kb on - strand, within BT0530 at 653.498 kb on + strand, within BT0530 at 653.609 kb on + strand, within BT0530 at 653.699 kb on + strand, within BT0530 at 653.700 kb on - strand, within BT0530 at 653.701 kb on + strand, within BT0530 at 653.701 kb on + strand, within BT0530 at 653.706 kb on - strand, within BT0530 at 653.729 kb on + strand, within BT0530 at 653.730 kb on - strand, within BT0530 at 653.731 kb on + strand, within BT0530 at 653.732 kb on - strand, within BT0530 at 653.879 kb on + strand, within BT0530 at 653.973 kb on - strand, within BT0530 at 653.995 kb on - strand, within BT0530 at 654.154 kb on - strand, within BT0530 at 654.191 kb on + strand, within BT0530
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 651,473 + BT0527 0.60 -1.6 651,474 - BT0527 0.60 +0.0 651,477 - BT0527 0.61 +0.0 651,484 - BT0527 0.62 -2.0 651,484 - BT0527 0.62 +0.0 651,498 + BT0527 0.63 -4.0 651,514 - BT0527 0.65 +0.0 651,514 - BT0527 0.65 +0.0 651,574 - BT0527 0.73 -3.1 651,674 + BT0527 0.86 -0.7 651,977 - BT0528 0.29 +0.0 652,021 + BT0528 0.36 -2.3 652,077 + BT0528 0.45 +0.0 652,156 + BT0528 0.57 +0.0 652,189 + BT0528 0.63 -2.6 652,190 - BT0528 0.63 -2.0 652,190 - BT0528 0.63 -1.0 652,350 + BT0528 0.88 -3.8 652,351 - BT0528 0.89 -5.6 652,395 + -1.7 652,448 - -2.6 652,449 + +0.0 652,453 + -1.0 652,454 - -2.0 652,645 - BT0529 0.25 +0.0 652,709 + BT0529 0.33 +0.0 652,710 - BT0529 0.33 -1.6 652,710 - BT0529 0.33 +0.0 652,717 - BT0529 0.34 -2.0 652,857 + BT0529 0.52 -2.0 652,865 - BT0529 0.53 -2.3 652,890 + BT0529 0.56 -2.0 653,000 - BT0529 0.70 -1.6 653,000 - BT0529 0.70 -1.6 653,078 + BT0529 0.80 -2.3 653,114 + BT0529 0.85 +0.0 653,142 - -2.3 653,169 - +0.0 653,205 - +0.0 653,224 - -3.0 653,304 + -1.6 653,305 - +0.0 653,307 + -4.6 653,312 - +2.8 653,424 + BT0530 0.11 -4.6 653,451 - BT0530 0.14 -2.3 653,498 + BT0530 0.19 +0.0 653,609 + BT0530 0.30 -1.6 653,699 + BT0530 0.39 -2.6 653,700 - BT0530 0.39 -0.6 653,701 + BT0530 0.39 -1.6 653,701 + BT0530 0.39 -1.6 653,706 - BT0530 0.39 -2.8 653,729 + BT0530 0.42 -1.0 653,730 - BT0530 0.42 -2.3 653,731 + BT0530 0.42 -4.2 653,732 - BT0530 0.42 +0.0 653,879 + BT0530 0.57 -1.6 653,973 - BT0530 0.66 -2.6 653,995 - BT0530 0.68 -2.0 654,154 - BT0530 0.84 -1.0 654,191 + BT0530 0.88 -2.3
Or see this region's nucleotide sequence