Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0518 and BT0519 are separated by 203 nucleotides BT0519 and BT0520 overlap by 11 nucleotides
BT0518: BT0518 - Bifunctional purine biosynthesis protein, putative 5-aminoimidazole-4-carboxamide ribonucleotide(AICAR) transformylase/IMP cyclohydrolase (NCBI ptt file), at 640,445 to 641,620
BT0518
BT0519: BT0519 - hypothetical protein (NCBI ptt file), at 641,824 to 642,528
BT0519
BT0520: BT0520 - transcriptional regulator (NCBI ptt file), at 642,518 to 643,129
BT0520
Position (kb) 641
642
643 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 640.835 kb on - strand, within BT0518 at 640.971 kb on - strand, within BT0518 at 641.123 kb on + strand, within BT0518 at 641.123 kb on + strand, within BT0518 at 641.123 kb on + strand, within BT0518 at 641.214 kb on - strand, within BT0518 at 641.227 kb on + strand, within BT0518 at 641.290 kb on + strand, within BT0518 at 641.316 kb on + strand, within BT0518 at 641.342 kb on + strand, within BT0518 at 641.468 kb on - strand, within BT0518 at 641.471 kb on + strand, within BT0518 at 641.628 kb on - strand at 641.671 kb on - strand at 641.701 kb on + strand at 641.703 kb on + strand at 641.708 kb on + strand at 641.752 kb on + strand at 641.816 kb on - strand at 641.816 kb on - strand at 641.965 kb on - strand, within BT0519 at 641.973 kb on - strand, within BT0519 at 642.095 kb on - strand, within BT0519 at 642.168 kb on - strand, within BT0519 at 642.217 kb on - strand, within BT0519 at 642.254 kb on - strand, within BT0519 at 642.254 kb on - strand, within BT0519 at 642.371 kb on - strand at 642.423 kb on - strand, within BT0519 at 642.423 kb on - strand, within BT0519 at 642.425 kb on - strand, within BT0519 at 642.724 kb on - strand, within BT0520 at 642.812 kb on + strand, within BT0520 at 643.034 kb on - strand, within BT0520 at 643.079 kb on + strand at 643.093 kb on + strand at 643.244 kb on - strand at 643.328 kb on + strand at 643.333 kb on - strand at 643.457 kb on + strand at 643.482 kb on - strand at 643.485 kb on + strand at 643.486 kb on - strand at 643.486 kb on - strand at 643.486 kb on - strand at 643.501 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 640,835 - BT0518 0.33 -1.0 640,971 - BT0518 0.45 -0.6 641,123 + BT0518 0.58 +2.6 641,123 + BT0518 0.58 -2.5 641,123 + BT0518 0.58 -2.0 641,214 - BT0518 0.65 -1.2 641,227 + BT0518 0.66 +1.0 641,290 + BT0518 0.72 +0.0 641,316 + BT0518 0.74 -1.1 641,342 + BT0518 0.76 -0.2 641,468 - BT0518 0.87 -2.8 641,471 + BT0518 0.87 -4.3 641,628 - -0.8 641,671 - +0.0 641,701 + -2.5 641,703 + -1.5 641,708 + -0.4 641,752 + -3.5 641,816 - -0.2 641,816 - +0.0 641,965 - BT0519 0.20 +0.0 641,973 - BT0519 0.21 -0.2 642,095 - BT0519 0.38 -0.2 642,168 - BT0519 0.49 -2.0 642,217 - BT0519 0.56 -2.5 642,254 - BT0519 0.61 +1.0 642,254 - BT0519 0.61 -3.1 642,371 - -1.0 642,423 - BT0519 0.85 -2.0 642,423 - BT0519 0.85 -3.5 642,425 - BT0519 0.85 -1.0 642,724 - BT0520 0.34 -2.3 642,812 + BT0520 0.48 +0.0 643,034 - BT0520 0.84 +1.4 643,079 + -0.2 643,093 + +0.0 643,244 - -1.5 643,328 + -2.1 643,333 - +0.0 643,457 + -1.0 643,482 - -3.3 643,485 + -1.4 643,486 - -3.1 643,486 - -1.0 643,486 - -0.3 643,501 + -1.0
Or see this region's nucleotide sequence