Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0396 and BT0397 are separated by 5 nucleotides BT0397 and BT0398 are separated by 41 nucleotides BT0398 and BT0399 are separated by 12 nucleotides
BT0396: BT0396 - putative UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI ptt file), at 485,128 to 486,117
BT0396
BT0397: BT0397 - conserved hypothetical protein (NCBI ptt file), at 486,123 to 486,569
BT0397
BT0398: BT0398 - polysaccharide export outer membrane protein (NCBI ptt file), at 486,611 to 487,405
BT0398
BT0399: BT0399 - tyrosine-protein kinase ptk involved in exopolysaccharide biosynthesis (NCBI ptt file), at 487,418 to 488,563
BT0399
Position (kb)
486
487
488 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 485.628 kb on + strand, within BT0396 at 485.802 kb on - strand, within BT0396 at 485.925 kb on - strand, within BT0396 at 485.940 kb on + strand, within BT0396 at 486.028 kb on - strand at 486.049 kb on - strand at 486.084 kb on - strand at 486.305 kb on - strand, within BT0397 at 486.463 kb on + strand, within BT0397 at 486.534 kb on + strand at 486.693 kb on - strand, within BT0398 at 486.693 kb on - strand, within BT0398 at 486.714 kb on + strand, within BT0398 at 486.717 kb on + strand, within BT0398 at 486.720 kb on - strand, within BT0398 at 486.784 kb on - strand, within BT0398 at 486.793 kb on + strand, within BT0398 at 486.836 kb on - strand, within BT0398 at 486.847 kb on + strand, within BT0398 at 486.891 kb on - strand, within BT0398 at 487.015 kb on - strand, within BT0398 at 487.142 kb on - strand, within BT0398 at 487.150 kb on - strand, within BT0398 at 487.171 kb on + strand, within BT0398 at 487.283 kb on + strand, within BT0398 at 487.283 kb on + strand, within BT0398 at 487.399 kb on - strand at 487.497 kb on - strand at 487.519 kb on + strand at 487.537 kb on - strand, within BT0399 at 487.661 kb on - strand, within BT0399 at 487.771 kb on - strand, within BT0399 at 487.771 kb on - strand, within BT0399 at 487.894 kb on - strand, within BT0399 at 487.909 kb on + strand, within BT0399 at 487.909 kb on - strand, within BT0399 at 487.917 kb on + strand, within BT0399 at 487.925 kb on - strand, within BT0399 at 487.929 kb on + strand, within BT0399 at 488.077 kb on + strand, within BT0399 at 488.080 kb on - strand, within BT0399 at 488.137 kb on + strand, within BT0399 at 488.312 kb on - strand, within BT0399 at 488.375 kb on - strand, within BT0399
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 485,628 + BT0396 0.51 -2.8 485,802 - BT0396 0.68 +0.0 485,925 - BT0396 0.81 +0.0 485,940 + BT0396 0.82 -2.0 486,028 - -2.3 486,049 - +0.0 486,084 - -1.5 486,305 - BT0397 0.41 -2.5 486,463 + BT0397 0.76 -1.0 486,534 + -2.3 486,693 - BT0398 0.10 +0.0 486,693 - BT0398 0.10 -3.9 486,714 + BT0398 0.13 +0.0 486,717 + BT0398 0.13 -1.0 486,720 - BT0398 0.14 -1.3 486,784 - BT0398 0.22 -1.0 486,793 + BT0398 0.23 -1.0 486,836 - BT0398 0.28 +2.0 486,847 + BT0398 0.30 +0.0 486,891 - BT0398 0.35 -2.0 487,015 - BT0398 0.51 -2.3 487,142 - BT0398 0.67 -1.5 487,150 - BT0398 0.68 -1.5 487,171 + BT0398 0.70 +0.0 487,283 + BT0398 0.85 -0.4 487,283 + BT0398 0.85 -1.5 487,399 - +1.0 487,497 - -2.5 487,519 + -1.0 487,537 - BT0399 0.10 -3.4 487,661 - BT0399 0.21 +0.3 487,771 - BT0399 0.31 +1.0 487,771 - BT0399 0.31 -1.5 487,894 - BT0399 0.42 -0.0 487,909 + BT0399 0.43 -2.0 487,909 - BT0399 0.43 -3.3 487,917 + BT0399 0.44 +0.9 487,925 - BT0399 0.44 +1.9 487,929 + BT0399 0.45 -0.2 488,077 + BT0399 0.58 -2.0 488,080 - BT0399 0.58 +0.0 488,137 + BT0399 0.63 -1.0 488,312 - BT0399 0.78 -2.0 488,375 - BT0399 0.84 -1.5
Or see this region's nucleotide sequence