Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0132 and BT0133 overlap by 17 nucleotides BT0133 and BT0134 are separated by 10 nucleotides BT0134 and BT0135 overlap by 23 nucleotides BT0135 and BT0136 are separated by 152 nucleotides
BT0132: BT0132 - alpha-glucosidase, putative (NCBI ptt file), at 127,918 to 129,852
BT0132
BT0133: BT0133 - putative sugar phosphate isomerase (NCBI ptt file), at 129,836 to 130,669
BT0133
BT0134: BT0134 - conserved hypothetical protein (NCBI ptt file), at 130,680 to 131,063
BT0134
BT0135: BT0135 - conserved hypothetical protein (NCBI ptt file), at 131,041 to 131,823
BT0135
BT0136: BT0136 - hypothetical protein (NCBI ptt file), at 131,976 to 133,586
BT0136
Position (kb) 130
131
132 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 129.703 kb on + strand at 129.703 kb on + strand at 129.705 kb on + strand at 129.706 kb on - strand at 129.718 kb on + strand at 129.742 kb on - strand at 129.742 kb on - strand at 129.790 kb on - strand at 129.887 kb on - strand at 130.009 kb on + strand, within BT0133 at 130.240 kb on - strand, within BT0133 at 130.276 kb on + strand at 130.277 kb on - strand, within BT0133 at 130.277 kb on - strand, within BT0133 at 130.291 kb on + strand, within BT0133 at 130.327 kb on + strand, within BT0133 at 130.394 kb on + strand, within BT0133 at 130.394 kb on + strand, within BT0133 at 130.395 kb on - strand, within BT0133 at 130.395 kb on - strand, within BT0133 at 130.408 kb on + strand, within BT0133 at 130.482 kb on - strand, within BT0133 at 130.482 kb on - strand, within BT0133 at 130.583 kb on - strand, within BT0133 at 130.681 kb on + strand at 130.689 kb on + strand at 130.716 kb on - strand at 130.822 kb on - strand, within BT0134 at 130.822 kb on - strand, within BT0134 at 130.838 kb on + strand, within BT0134 at 130.932 kb on + strand, within BT0134 at 130.933 kb on - strand, within BT0134 at 130.982 kb on + strand, within BT0134 at 131.044 kb on + strand at 131.045 kb on - strand at 131.077 kb on + strand at 131.083 kb on + strand at 131.124 kb on - strand, within BT0135 at 131.197 kb on + strand, within BT0135 at 131.206 kb on + strand, within BT0135 at 131.242 kb on - strand, within BT0135 at 131.401 kb on - strand, within BT0135 at 131.406 kb on + strand, within BT0135 at 131.419 kb on - strand, within BT0135 at 131.472 kb on + strand, within BT0135 at 131.489 kb on - strand, within BT0135 at 131.569 kb on + strand, within BT0135 at 131.570 kb on - strand, within BT0135 at 131.656 kb on + strand, within BT0135 at 131.726 kb on + strand, within BT0135 at 131.791 kb on - strand at 131.792 kb on + strand at 132.010 kb on + strand at 132.018 kb on - strand at 132.022 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM remove 129,703 + -2.1 129,703 + -1.3 129,705 + +0.1 129,706 - +0.1 129,718 + +0.9 129,742 - +0.1 129,742 - -1.5 129,790 - -1.3 129,887 - +0.1 130,009 + BT0133 0.21 -0.6 130,240 - BT0133 0.48 -2.3 130,276 + -0.9 130,277 - BT0133 0.53 -1.2 130,277 - BT0133 0.53 -1.3 130,291 + BT0133 0.55 -2.5 130,327 + BT0133 0.59 +0.5 130,394 + BT0133 0.67 +0.1 130,394 + BT0133 0.67 +0.1 130,395 - BT0133 0.67 -1.9 130,395 - BT0133 0.67 +1.1 130,408 + BT0133 0.69 -1.5 130,482 - BT0133 0.77 +0.3 130,482 - BT0133 0.77 +0.1 130,583 - BT0133 0.90 -0.9 130,681 + +0.1 130,689 + +0.1 130,716 - -2.5 130,822 - BT0134 0.37 -1.8 130,822 - BT0134 0.37 -1.8 130,838 + BT0134 0.41 +0.1 130,932 + BT0134 0.66 -2.9 130,933 - BT0134 0.66 -1.5 130,982 + BT0134 0.79 +0.1 131,044 + -3.5 131,045 - +1.1 131,077 + -1.9 131,083 + -0.9 131,124 - BT0135 0.11 -0.9 131,197 + BT0135 0.20 -0.9 131,206 + BT0135 0.21 -1.1 131,242 - BT0135 0.26 -1.1 131,401 - BT0135 0.46 +0.1 131,406 + BT0135 0.47 -3.1 131,419 - BT0135 0.48 -0.9 131,472 + BT0135 0.55 -0.9 131,489 - BT0135 0.57 +0.1 131,569 + BT0135 0.67 -3.6 131,570 - BT0135 0.68 -2.5 131,656 + BT0135 0.79 +0.1 131,726 + BT0135 0.87 +0.1 131,791 - -0.9 131,792 + +0.1 132,010 + +0.1 132,018 - +1.1 132,022 + -2.1
Or see this region's nucleotide sequence