Experiment: adipate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS32635 and RR42_RS32640 are separated by 38 nucleotides RR42_RS32640 and RR42_RS32645 are separated by 32 nucleotides RR42_RS32645 and RR42_RS32650 are separated by 8 nucleotides
RR42_RS32635: RR42_RS32635 - 4-oxalocrotonate tautomerase, at 2,719,552 to 2,719,743
_RS32635
RR42_RS32640: RR42_RS32640 - 4-oxalocrotonate decarboxylase, at 2,719,782 to 2,720,570
_RS32640
RR42_RS32645: RR42_RS32645 - 2-keto-4-pentenoate hydratase, at 2,720,603 to 2,721,385
_RS32645
RR42_RS32650: RR42_RS32650 - betaine-aldehyde dehydrogenase, at 2,721,394 to 2,722,848
_RS32650
Position (kb)
2720
2721
2722 Strain fitness (log2 ratio)
-1
0
1 at 2720.589 kb on + strand at 2721.265 kb on + strand, within RR42_RS32645 at 2721.271 kb on - strand, within RR42_RS32645 at 2721.371 kb on - strand at 2721.428 kb on + strand at 2721.428 kb on + strand at 2721.663 kb on + strand, within RR42_RS32650 at 2721.830 kb on + strand, within RR42_RS32650 at 2721.972 kb on - strand, within RR42_RS32650 at 2721.972 kb on - strand, within RR42_RS32650 at 2722.111 kb on + strand, within RR42_RS32650 at 2722.149 kb on + strand, within RR42_RS32650 at 2722.244 kb on + strand, within RR42_RS32650
Per-strain Table
Position Strand Gene LocusTag Fraction adipate (C) remove 2,720,589 + +0.6 2,721,265 + RR42_RS32645 0.85 +0.3 2,721,271 - RR42_RS32645 0.85 -1.5 2,721,371 - +0.5 2,721,428 + +0.6 2,721,428 + +1.1 2,721,663 + RR42_RS32650 0.18 -0.5 2,721,830 + RR42_RS32650 0.30 -0.7 2,721,972 - RR42_RS32650 0.40 -0.8 2,721,972 - RR42_RS32650 0.40 -0.5 2,722,111 + RR42_RS32650 0.49 -0.8 2,722,149 + RR42_RS32650 0.52 +1.3 2,722,244 + RR42_RS32650 0.58 -0.5
Or see this region's nucleotide sequence