Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22720

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 83.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22715 and BWI76_RS22720 overlap by 1 nucleotidesBWI76_RS22720 and BWI76_RS22725 overlap by 4 nucleotidesBWI76_RS22725 and BWI76_RS22730 overlap by 19 nucleotides BWI76_RS22715: BWI76_RS22715 - type I-E CRISPR-associated endoribonuclease Cas2, at 4,734,507 to 4,734,800 _RS22715 BWI76_RS22720: BWI76_RS22720 - subtype I-E CRISPR-associated endonuclease Cas1, at 4,734,800 to 4,735,720 _RS22720 BWI76_RS22725: BWI76_RS22725 - type I-E CRISPR-associated protein Cas6/Cse3/CasE, at 4,735,717 to 4,736,367 _RS22725 BWI76_RS22730: BWI76_RS22730 - type I-E CRISPR-associated protein Cas5/CasD, at 4,736,349 to 4,737,095 _RS22730 Position (kb) 4734 4735 4736Strain fitness (log2 ratio) -1 0 1 2at 4733.939 kb on + strandat 4733.976 kb on + strandat 4734.083 kb on + strandat 4734.254 kb on + strandat 4734.286 kb on + strandat 4734.286 kb on + strandat 4734.315 kb on - strandat 4734.467 kb on + strandat 4734.565 kb on - strand, within BWI76_RS22715at 4734.565 kb on - strand, within BWI76_RS22715at 4734.619 kb on + strand, within BWI76_RS22715at 4734.632 kb on - strand, within BWI76_RS22715at 4734.632 kb on - strand, within BWI76_RS22715at 4734.632 kb on - strand, within BWI76_RS22715at 4734.876 kb on + strandat 4734.876 kb on + strandat 4734.886 kb on + strandat 4734.943 kb on + strand, within BWI76_RS22720at 4734.943 kb on + strand, within BWI76_RS22720at 4734.943 kb on + strand, within BWI76_RS22720at 4734.951 kb on - strand, within BWI76_RS22720at 4734.951 kb on - strand, within BWI76_RS22720at 4734.951 kb on - strand, within BWI76_RS22720at 4734.951 kb on - strand, within BWI76_RS22720at 4735.143 kb on + strand, within BWI76_RS22720at 4735.170 kb on - strand, within BWI76_RS22720at 4735.189 kb on + strand, within BWI76_RS22720at 4735.214 kb on + strand, within BWI76_RS22720at 4735.243 kb on + strand, within BWI76_RS22720at 4735.251 kb on - strand, within BWI76_RS22720at 4735.263 kb on + strand, within BWI76_RS22720at 4735.297 kb on + strand, within BWI76_RS22720at 4735.297 kb on + strand, within BWI76_RS22720at 4735.305 kb on - strand, within BWI76_RS22720at 4735.305 kb on - strand, within BWI76_RS22720at 4735.305 kb on - strand, within BWI76_RS22720at 4735.335 kb on + strand, within BWI76_RS22720at 4735.708 kb on + strandat 4735.708 kb on + strandat 4735.751 kb on - strandat 4735.751 kb on - strandat 4735.808 kb on - strand, within BWI76_RS22725at 4735.847 kb on + strand, within BWI76_RS22725at 4735.855 kb on - strand, within BWI76_RS22725at 4735.865 kb on - strand, within BWI76_RS22725at 4735.958 kb on + strand, within BWI76_RS22725at 4736.003 kb on + strand, within BWI76_RS22725at 4736.099 kb on - strand, within BWI76_RS22725at 4736.099 kb on - strand, within BWI76_RS22725at 4736.104 kb on + strand, within BWI76_RS22725at 4736.104 kb on + strand, within BWI76_RS22725at 4736.183 kb on - strand, within BWI76_RS22725at 4736.187 kb on + strand, within BWI76_RS22725at 4736.207 kb on + strand, within BWI76_RS22725at 4736.329 kb on - strandat 4736.362 kb on + strandat 4736.370 kb on - strandat 4736.462 kb on - strand, within BWI76_RS22730at 4736.463 kb on - strand, within BWI76_RS22730at 4736.574 kb on + strand, within BWI76_RS22730at 4736.680 kb on - strand, within BWI76_RS22730

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x; and D-Glucose 83.25 mM
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4,733,939 + +0.3
4,733,976 + +0.2
4,734,083 + +1.1
4,734,254 + -0.4
4,734,286 + +1.2
4,734,286 + -0.7
4,734,315 - -1.4
4,734,467 + +0.5
4,734,565 - BWI76_RS22715 0.20 +0.0
4,734,565 - BWI76_RS22715 0.20 -0.8
4,734,619 + BWI76_RS22715 0.38 -0.3
4,734,632 - BWI76_RS22715 0.43 -0.4
4,734,632 - BWI76_RS22715 0.43 +0.6
4,734,632 - BWI76_RS22715 0.43 -0.0
4,734,876 + +0.6
4,734,876 + -0.2
4,734,886 + +0.6
4,734,943 + BWI76_RS22720 0.16 +0.2
4,734,943 + BWI76_RS22720 0.16 +0.2
4,734,943 + BWI76_RS22720 0.16 +0.4
4,734,951 - BWI76_RS22720 0.16 +0.9
4,734,951 - BWI76_RS22720 0.16 +0.4
4,734,951 - BWI76_RS22720 0.16 +0.8
4,734,951 - BWI76_RS22720 0.16 +1.0
4,735,143 + BWI76_RS22720 0.37 +1.2
4,735,170 - BWI76_RS22720 0.40 +0.8
4,735,189 + BWI76_RS22720 0.42 +0.4
4,735,214 + BWI76_RS22720 0.45 +0.1
4,735,243 + BWI76_RS22720 0.48 -0.2
4,735,251 - BWI76_RS22720 0.49 -0.1
4,735,263 + BWI76_RS22720 0.50 +0.2
4,735,297 + BWI76_RS22720 0.54 +0.4
4,735,297 + BWI76_RS22720 0.54 +0.6
4,735,305 - BWI76_RS22720 0.55 +2.0
4,735,305 - BWI76_RS22720 0.55 +0.7
4,735,305 - BWI76_RS22720 0.55 -1.0
4,735,335 + BWI76_RS22720 0.58 +1.0
4,735,708 + +0.5
4,735,708 + -0.4
4,735,751 - -0.2
4,735,751 - +0.7
4,735,808 - BWI76_RS22725 0.14 +0.3
4,735,847 + BWI76_RS22725 0.20 -0.1
4,735,855 - BWI76_RS22725 0.21 -0.8
4,735,865 - BWI76_RS22725 0.23 +0.4
4,735,958 + BWI76_RS22725 0.37 -0.1
4,736,003 + BWI76_RS22725 0.44 -0.2
4,736,099 - BWI76_RS22725 0.59 +0.0
4,736,099 - BWI76_RS22725 0.59 -0.2
4,736,104 + BWI76_RS22725 0.59 -0.4
4,736,104 + BWI76_RS22725 0.59 +0.3
4,736,183 - BWI76_RS22725 0.72 -0.1
4,736,187 + BWI76_RS22725 0.72 -0.2
4,736,207 + BWI76_RS22725 0.75 -0.6
4,736,329 - +0.3
4,736,362 + -1.0
4,736,370 - +0.1
4,736,462 - BWI76_RS22730 0.15 +0.1
4,736,463 - BWI76_RS22730 0.15 +0.7
4,736,574 + BWI76_RS22730 0.30 +0.2
4,736,680 - BWI76_RS22730 0.44 -0.4

Or see this region's nucleotide sequence