Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21160 and BWI76_RS21165 are separated by 177 nucleotides BWI76_RS21165 and BWI76_RS21170 are separated by 233 nucleotides BWI76_RS21170 and BWI76_RS21175 are separated by 120 nucleotides BWI76_RS21175 and BWI76_RS21180 are separated by 54 nucleotides
BWI76_RS21160: BWI76_RS21160 - IscS subfamily cysteine desulfurase, at 4,430,056 to 4,431,270
_RS21160
BWI76_RS21165: BWI76_RS21165 - Fe-S cluster assembly transcriptional regulator IscR, at 4,431,448 to 4,431,939
_RS21165
BWI76_RS21170: BWI76_RS21170 - tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ, at 4,432,173 to 4,432,904
_RS21170
BWI76_RS21175: BWI76_RS21175 - inositol monophosphatase, at 4,433,025 to 4,433,828
_RS21175
BWI76_RS21180: BWI76_RS21180 - nickel transporter, at 4,433,883 to 4,434,863
_RS21180
Position (kb)
4432
4433 Strain fitness (log2 ratio)
-1
0
1 at 4431.572 kb on - strand, within BWI76_RS21165 at 4432.156 kb on + strand at 4432.639 kb on + strand, within BWI76_RS21170 at 4432.647 kb on - strand, within BWI76_RS21170 at 4432.809 kb on - strand, within BWI76_RS21170 at 4432.820 kb on - strand, within BWI76_RS21170 at 4432.851 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x; and D-Glucose 55.5 mM remove 4,431,572 - BWI76_RS21165 0.25 -0.1 4,432,156 + -0.5 4,432,639 + BWI76_RS21170 0.64 -1.2 4,432,647 - BWI76_RS21170 0.65 -0.1 4,432,809 - BWI76_RS21170 0.87 -0.4 4,432,820 - BWI76_RS21170 0.88 -0.8 4,432,851 - -0.4
Or see this region's nucleotide sequence