Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27675

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27670 and BWI76_RS27675 overlap by 4 nucleotidesBWI76_RS27675 and BWI76_RS27680 are separated by 80 nucleotidesBWI76_RS27680 and BWI76_RS27685 are separated by 148 nucleotides BWI76_RS27670: BWI76_RS27670 - LysR family transcriptional regulator, at 5,740,075 to 5,740,989 _RS27670 BWI76_RS27675: BWI76_RS27675 - EmrB/QacA family drug resistance transporter, at 5,740,986 to 5,742,482 _RS27675 BWI76_RS27680: BWI76_RS27680 - TetR family transcriptional regulator, at 5,742,563 to 5,743,210 _RS27680 BWI76_RS27685: BWI76_RS27685 - N-acetyltransferase, at 5,743,359 to 5,743,775 _RS27685 Position (kb) 5740 5741 5742 5743Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6 7at 5740.167 kb on - strand, within BWI76_RS27670at 5740.297 kb on + strand, within BWI76_RS27670at 5740.356 kb on + strand, within BWI76_RS27670at 5740.453 kb on - strand, within BWI76_RS27670at 5740.884 kb on + strand, within BWI76_RS27670at 5740.884 kb on + strand, within BWI76_RS27670at 5740.910 kb on + strandat 5740.918 kb on - strandat 5740.968 kb on + strandat 5740.999 kb on + strandat 5741.031 kb on - strandat 5741.141 kb on - strand, within BWI76_RS27675at 5741.217 kb on + strand, within BWI76_RS27675at 5741.287 kb on - strand, within BWI76_RS27675at 5741.296 kb on + strand, within BWI76_RS27675at 5741.304 kb on - strand, within BWI76_RS27675at 5741.417 kb on - strand, within BWI76_RS27675at 5741.556 kb on + strand, within BWI76_RS27675at 5741.561 kb on + strand, within BWI76_RS27675at 5741.584 kb on + strand, within BWI76_RS27675at 5741.594 kb on + strand, within BWI76_RS27675at 5741.841 kb on + strand, within BWI76_RS27675at 5742.099 kb on - strand, within BWI76_RS27675at 5742.169 kb on - strand, within BWI76_RS27675at 5742.284 kb on - strand, within BWI76_RS27675at 5742.336 kb on - strandat 5742.477 kb on + strandat 5742.654 kb on + strand, within BWI76_RS27680at 5742.668 kb on - strand, within BWI76_RS27680at 5743.065 kb on + strand, within BWI76_RS27680at 5743.073 kb on - strand, within BWI76_RS27680at 5743.073 kb on - strand, within BWI76_RS27680at 5743.073 kb on - strand, within BWI76_RS27680at 5743.080 kb on + strand, within BWI76_RS27680at 5743.170 kb on + strandat 5743.178 kb on - strandat 5743.229 kb on + strandat 5743.240 kb on + strandat 5743.247 kb on + strandat 5743.354 kb on - strandat 5743.354 kb on - strandat 5743.396 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM
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5,740,167 - BWI76_RS27670 0.10 -0.6
5,740,297 + BWI76_RS27670 0.24 -0.1
5,740,356 + BWI76_RS27670 0.31 -0.6
5,740,453 - BWI76_RS27670 0.41 -0.5
5,740,884 + BWI76_RS27670 0.88 +2.2
5,740,884 + BWI76_RS27670 0.88 -0.5
5,740,910 + -0.1
5,740,918 - -0.7
5,740,968 + -0.0
5,740,999 + +4.0
5,741,031 - +1.2
5,741,141 - BWI76_RS27675 0.10 +5.1
5,741,217 + BWI76_RS27675 0.15 +6.0
5,741,287 - BWI76_RS27675 0.20 +5.1
5,741,296 + BWI76_RS27675 0.21 +6.2
5,741,304 - BWI76_RS27675 0.21 +5.0
5,741,417 - BWI76_RS27675 0.29 +6.1
5,741,556 + BWI76_RS27675 0.38 +6.5
5,741,561 + BWI76_RS27675 0.38 +6.4
5,741,584 + BWI76_RS27675 0.40 +5.7
5,741,594 + BWI76_RS27675 0.41 +6.4
5,741,841 + BWI76_RS27675 0.57 +5.9
5,742,099 - BWI76_RS27675 0.74 +6.5
5,742,169 - BWI76_RS27675 0.79 +7.4
5,742,284 - BWI76_RS27675 0.87 +6.5
5,742,336 - +6.0
5,742,477 + +5.1
5,742,654 + BWI76_RS27680 0.14 +0.5
5,742,668 - BWI76_RS27680 0.16 -2.7
5,743,065 + BWI76_RS27680 0.77 -0.5
5,743,073 - BWI76_RS27680 0.79 -3.1
5,743,073 - BWI76_RS27680 0.79 -0.1
5,743,073 - BWI76_RS27680 0.79 +0.1
5,743,080 + BWI76_RS27680 0.80 +0.5
5,743,170 + -3.5
5,743,178 - -0.8
5,743,229 + -1.8
5,743,240 + +0.1
5,743,247 + -0.9
5,743,354 - -1.5
5,743,354 - +0.4
5,743,396 - +0.7

Or see this region's nucleotide sequence