Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15200

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15190 and BWI76_RS15195 are separated by 107 nucleotidesBWI76_RS15195 and BWI76_RS15200 overlap by 1 nucleotidesBWI76_RS15200 and BWI76_RS15205 are separated by 204 nucleotides BWI76_RS15190: BWI76_RS15190 - GGDEF domain-containing protein, at 3,184,118 to 3,185,530 _RS15190 BWI76_RS15195: BWI76_RS15195 - DUF4186 domain-containing protein, at 3,185,638 to 3,186,012 _RS15195 BWI76_RS15200: BWI76_RS15200 - glutaminase, at 3,186,012 to 3,186,938 _RS15200 BWI76_RS15205: BWI76_RS15205 - succinate-semialdehyde dehydrogenase, at 3,187,143 to 3,188,528 _RS15205 Position (kb) 3186 3187Strain fitness (log2 ratio) -1 0 1at 3185.286 kb on - strand, within BWI76_RS15190at 3185.294 kb on - strand, within BWI76_RS15190at 3185.446 kb on + strandat 3185.446 kb on + strandat 3185.543 kb on + strandat 3185.710 kb on - strand, within BWI76_RS15195at 3185.881 kb on - strand, within BWI76_RS15195at 3186.093 kb on - strandat 3186.326 kb on + strand, within BWI76_RS15200at 3186.539 kb on - strand, within BWI76_RS15200at 3187.198 kb on - strandat 3187.473 kb on + strand, within BWI76_RS15205at 3187.555 kb on - strand, within BWI76_RS15205

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM
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3,185,286 - BWI76_RS15190 0.83 +1.3
3,185,294 - BWI76_RS15190 0.83 +1.0
3,185,446 + +1.5
3,185,446 + +0.6
3,185,543 + +0.8
3,185,710 - BWI76_RS15195 0.19 -0.0
3,185,881 - BWI76_RS15195 0.65 -0.6
3,186,093 - -0.2
3,186,326 + BWI76_RS15200 0.34 +0.3
3,186,539 - BWI76_RS15200 0.57 -0.7
3,187,198 - -0.4
3,187,473 + BWI76_RS15205 0.24 -0.4
3,187,555 - BWI76_RS15205 0.30 +0.9

Or see this region's nucleotide sequence