Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13110 and BWI76_RS13115 overlap by 4 nucleotides BWI76_RS13115 and BWI76_RS13120 are separated by 2 nucleotides BWI76_RS13120 and BWI76_RS13125 are separated by 3 nucleotides
BWI76_RS13110: BWI76_RS13110 - phenylacetic acid degradation protein, at 2,734,299 to 2,735,369
_RS13110
BWI76_RS13115: BWI76_RS13115 - 2,3-dehydroadipyl-CoA hydratase, at 2,735,366 to 2,736,133
_RS13115
BWI76_RS13120: BWI76_RS13120 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase, at 2,736,136 to 2,736,924
_RS13120
BWI76_RS13125: BWI76_RS13125 - 3-hydroxyacyl-CoA dehydrogenase, at 2,736,928 to 2,738,349
_RS13125
Position (kb)
2736
2737 Strain fitness (log2 ratio)
-1
0
1 at 2735.337 kb on + strand at 2735.375 kb on - strand at 2736.150 kb on - strand at 2736.166 kb on - strand at 2736.758 kb on + strand, within BWI76_RS13120 at 2736.963 kb on - strand at 2737.662 kb on + strand, within BWI76_RS13125 at 2737.786 kb on + strand, within BWI76_RS13125 at 2737.794 kb on - strand, within BWI76_RS13125 at 2737.877 kb on + strand, within BWI76_RS13125
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x; and D-Glucose 37 mM remove 2,735,337 + +0.3 2,735,375 - -0.0 2,736,150 - -0.3 2,736,166 - +0.3 2,736,758 + BWI76_RS13120 0.79 -0.7 2,736,963 - -0.6 2,737,662 + BWI76_RS13125 0.52 -0.3 2,737,786 + BWI76_RS13125 0.60 -0.6 2,737,794 - BWI76_RS13125 0.61 -1.3 2,737,877 + BWI76_RS13125 0.67 -0.7
Or see this region's nucleotide sequence