Experiment: Varel_Bryant_medium_Glucose with Cephalothin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1414 and BT1415 are separated by 7 nucleotides BT1415 and BT1416 overlap by 4 nucleotides BT1416 and BT1417 are separated by 11 nucleotides
BT1414: BT1414 - hypothetical protein (NCBI ptt file), at 1,746,996 to 1,748,282
BT1414
BT1415: BT1415 - cytochrome c biogenesis protein ccsA (NCBI ptt file), at 1,748,290 to 1,749,081
BT1415
BT1416: BT1416 - conserved hypothetical protein (NCBI ptt file), at 1,749,078 to 1,750,313
BT1416
BT1417: BT1417 - cytochrome C552 precursor (NCBI ptt file), at 1,750,325 to 1,751,806
BT1417
Position (kb)
1749
1750
1751 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1748.148 kb on + strand, within BT1414 at 1748.247 kb on + strand at 1748.252 kb on + strand at 1748.252 kb on + strand at 1748.253 kb on - strand at 1748.301 kb on + strand at 1748.302 kb on - strand at 1748.307 kb on + strand at 1748.308 kb on - strand at 1748.312 kb on - strand at 1748.366 kb on - strand at 1748.366 kb on - strand at 1748.366 kb on - strand at 1748.367 kb on - strand at 1748.367 kb on - strand at 1748.383 kb on + strand, within BT1415 at 1748.384 kb on - strand, within BT1415 at 1748.439 kb on + strand, within BT1415 at 1748.439 kb on + strand, within BT1415 at 1748.440 kb on - strand, within BT1415 at 1748.440 kb on - strand, within BT1415 at 1748.442 kb on - strand, within BT1415 at 1748.442 kb on - strand, within BT1415 at 1748.443 kb on + strand, within BT1415 at 1748.443 kb on + strand, within BT1415 at 1748.444 kb on - strand, within BT1415 at 1748.444 kb on - strand, within BT1415 at 1748.463 kb on + strand, within BT1415 at 1748.464 kb on - strand, within BT1415 at 1748.501 kb on + strand, within BT1415 at 1748.502 kb on - strand, within BT1415 at 1748.556 kb on + strand, within BT1415 at 1748.556 kb on + strand, within BT1415 at 1748.556 kb on + strand, within BT1415 at 1748.573 kb on + strand, within BT1415 at 1748.675 kb on + strand at 1748.685 kb on + strand, within BT1415 at 1748.685 kb on + strand, within BT1415 at 1748.686 kb on - strand, within BT1415 at 1748.686 kb on - strand, within BT1415 at 1748.687 kb on + strand, within BT1415 at 1748.687 kb on + strand, within BT1415 at 1748.688 kb on - strand, within BT1415 at 1748.688 kb on - strand, within BT1415 at 1748.688 kb on - strand, within BT1415 at 1748.692 kb on + strand, within BT1415 at 1748.692 kb on + strand, within BT1415 at 1748.693 kb on - strand, within BT1415 at 1748.778 kb on + strand, within BT1415 at 1748.778 kb on + strand, within BT1415 at 1748.779 kb on - strand, within BT1415 at 1748.779 kb on - strand, within BT1415 at 1748.779 kb on - strand, within BT1415 at 1748.783 kb on - strand, within BT1415 at 1748.822 kb on + strand, within BT1415 at 1748.837 kb on + strand, within BT1415 at 1748.838 kb on - strand, within BT1415 at 1748.873 kb on + strand, within BT1415 at 1749.061 kb on + strand at 1749.160 kb on + strand at 1749.160 kb on + strand at 1749.160 kb on + strand at 1749.161 kb on - strand at 1749.163 kb on - strand at 1749.165 kb on + strand at 1749.166 kb on - strand at 1749.166 kb on - strand at 1749.166 kb on - strand at 1749.235 kb on + strand, within BT1416 at 1749.240 kb on - strand, within BT1416 at 1749.264 kb on + strand, within BT1416 at 1749.282 kb on + strand, within BT1416 at 1749.282 kb on + strand, within BT1416 at 1749.283 kb on - strand, within BT1416 at 1749.330 kb on + strand, within BT1416 at 1749.407 kb on - strand, within BT1416 at 1749.482 kb on - strand, within BT1416 at 1749.491 kb on - strand, within BT1416 at 1749.543 kb on + strand, within BT1416 at 1749.564 kb on - strand, within BT1416 at 1749.743 kb on - strand, within BT1416 at 1749.837 kb on - strand, within BT1416 at 1749.864 kb on + strand, within BT1416 at 1749.886 kb on - strand, within BT1416 at 1749.886 kb on - strand, within BT1416 at 1749.937 kb on - strand, within BT1416 at 1750.004 kb on + strand, within BT1416 at 1750.005 kb on - strand, within BT1416 at 1750.005 kb on - strand, within BT1416 at 1750.123 kb on + strand, within BT1416 at 1750.124 kb on - strand, within BT1416 at 1750.167 kb on - strand, within BT1416 at 1750.251 kb on + strand at 1750.289 kb on + strand at 1750.289 kb on + strand at 1750.313 kb on + strand at 1750.313 kb on + strand at 1750.402 kb on + strand at 1750.437 kb on + strand at 1750.438 kb on - strand at 1750.776 kb on + strand, within BT1417 at 1750.777 kb on - strand, within BT1417 at 1750.786 kb on + strand, within BT1417 at 1750.858 kb on + strand, within BT1417 at 1750.895 kb on - strand, within BT1417 at 1750.955 kb on + strand, within BT1417 at 1751.013 kb on + strand, within BT1417 at 1751.014 kb on - strand, within BT1417 at 1751.014 kb on - strand, within BT1417 at 1751.019 kb on + strand, within BT1417 at 1751.040 kb on + strand, within BT1417 at 1751.091 kb on + strand, within BT1417 at 1751.120 kb on + strand, within BT1417 at 1751.121 kb on - strand, within BT1417 at 1751.165 kb on - strand, within BT1417 at 1751.224 kb on + strand, within BT1417 at 1751.313 kb on + strand, within BT1417 at 1751.313 kb on + strand, within BT1417
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Cephalothin 0.1 mM remove 1,748,148 + BT1414 0.90 -0.0 1,748,247 + +1.2 1,748,252 + -0.6 1,748,252 + -0.6 1,748,253 - -2.0 1,748,301 + -1.0 1,748,302 - -1.6 1,748,307 + +2.3 1,748,308 - -0.0 1,748,312 - -0.0 1,748,366 - -1.0 1,748,366 - -1.0 1,748,366 - +0.4 1,748,367 - +1.6 1,748,367 - -0.9 1,748,383 + BT1415 0.12 -0.5 1,748,384 - BT1415 0.12 +1.0 1,748,439 + BT1415 0.19 -0.6 1,748,439 + BT1415 0.19 -1.9 1,748,440 - BT1415 0.19 -2.2 1,748,440 - BT1415 0.19 -2.6 1,748,442 - BT1415 0.19 -1.6 1,748,442 - BT1415 0.19 -2.0 1,748,443 + BT1415 0.19 -1.6 1,748,443 + BT1415 0.19 -2.3 1,748,444 - BT1415 0.19 -1.6 1,748,444 - BT1415 0.19 -1.1 1,748,463 + BT1415 0.22 -0.6 1,748,464 - BT1415 0.22 -0.0 1,748,501 + BT1415 0.27 -0.4 1,748,502 - BT1415 0.27 -0.3 1,748,556 + BT1415 0.34 -0.0 1,748,556 + BT1415 0.34 -1.0 1,748,556 + BT1415 0.34 -1.3 1,748,573 + BT1415 0.36 +1.6 1,748,675 + -1.0 1,748,685 + BT1415 0.50 -2.8 1,748,685 + BT1415 0.50 -1.2 1,748,686 - BT1415 0.50 -2.3 1,748,686 - BT1415 0.50 -2.3 1,748,687 + BT1415 0.50 -2.2 1,748,687 + BT1415 0.50 -3.0 1,748,688 - BT1415 0.50 +1.0 1,748,688 - BT1415 0.50 -0.0 1,748,688 - BT1415 0.50 -0.0 1,748,692 + BT1415 0.51 -1.6 1,748,692 + BT1415 0.51 -1.0 1,748,693 - BT1415 0.51 +2.3 1,748,778 + BT1415 0.62 +0.8 1,748,778 + BT1415 0.62 -1.3 1,748,779 - BT1415 0.62 -1.0 1,748,779 - BT1415 0.62 -0.0 1,748,779 - BT1415 0.62 -0.0 1,748,783 - BT1415 0.62 -0.0 1,748,822 + BT1415 0.67 -1.0 1,748,837 + BT1415 0.69 -1.0 1,748,838 - BT1415 0.69 -0.0 1,748,873 + BT1415 0.74 -0.6 1,749,061 + +0.8 1,749,160 + +1.0 1,749,160 + -0.5 1,749,160 + -1.6 1,749,161 - -0.0 1,749,163 - -1.6 1,749,165 + -1.0 1,749,166 - +0.4 1,749,166 - -0.0 1,749,166 - -1.5 1,749,235 + BT1416 0.13 -2.8 1,749,240 - BT1416 0.13 -0.0 1,749,264 + BT1416 0.15 -2.0 1,749,282 + BT1416 0.17 -1.3 1,749,282 + BT1416 0.17 -1.2 1,749,283 - BT1416 0.17 -2.2 1,749,330 + BT1416 0.20 -1.0 1,749,407 - BT1416 0.27 -1.0 1,749,482 - BT1416 0.33 +2.6 1,749,491 - BT1416 0.33 -1.6 1,749,543 + BT1416 0.38 -0.9 1,749,564 - BT1416 0.39 +0.6 1,749,743 - BT1416 0.54 -1.6 1,749,837 - BT1416 0.61 -0.4 1,749,864 + BT1416 0.64 -1.6 1,749,886 - BT1416 0.65 -0.0 1,749,886 - BT1416 0.65 -2.5 1,749,937 - BT1416 0.69 -0.8 1,750,004 + BT1416 0.75 -1.0 1,750,005 - BT1416 0.75 -1.6 1,750,005 - BT1416 0.75 +1.0 1,750,123 + BT1416 0.85 -1.6 1,750,124 - BT1416 0.85 -0.0 1,750,167 - BT1416 0.88 -1.4 1,750,251 + +0.6 1,750,289 + +2.0 1,750,289 + -1.3 1,750,313 + -1.0 1,750,313 + -2.3 1,750,402 + -0.8 1,750,437 + -2.0 1,750,438 - +1.0 1,750,776 + BT1417 0.30 +1.6 1,750,777 - BT1417 0.30 +0.6 1,750,786 + BT1417 0.31 -0.4 1,750,858 + BT1417 0.36 +0.4 1,750,895 - BT1417 0.38 -1.0 1,750,955 + BT1417 0.43 -0.0 1,751,013 + BT1417 0.46 -2.7 1,751,014 - BT1417 0.46 -1.6 1,751,014 - BT1417 0.46 -1.2 1,751,019 + BT1417 0.47 -0.6 1,751,040 + BT1417 0.48 -0.0 1,751,091 + BT1417 0.52 -0.9 1,751,120 + BT1417 0.54 +1.0 1,751,121 - BT1417 0.54 -0.0 1,751,165 - BT1417 0.57 -1.6 1,751,224 + BT1417 0.61 -0.0 1,751,313 + BT1417 0.67 -0.0 1,751,313 + BT1417 0.67 +2.0
Or see this region's nucleotide sequence