Experiment: MoYLS4 with Uranyl Acetate 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrK and pyrD are separated by 8 nucleotides pyrD and DVU3318 are separated by 183 nucleotides DVU3318 and putA are separated by 94 nucleotides
DVU3315: pyrK - dihydroorotate dehydrogenase, electron transfer subunit (TIGR), at 3,493,097 to 3,493,915
pyrK
DVU3316: pyrD - dihydroorotate dehydrogenase (TIGR), at 3,493,924 to 3,494,847
pyrD
DVU3318: DVU3318 - hypothetical protein (TIGR), at 3,495,031 to 3,495,201
DVU3318
DVU3319: putA - proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR), at 3,495,296 to 3,498,316
putA
Position (kb)
3493
3494
3495 Strain fitness (log2 ratio)
-1
0
1
2 at 3492.984 kb on - strand at 3493.009 kb on + strand at 3493.017 kb on - strand at 3493.033 kb on + strand at 3494.842 kb on + strand at 3495.047 kb on - strand at 3495.075 kb on - strand, within DVU3318 at 3495.098 kb on - strand, within DVU3318 at 3495.117 kb on - strand, within DVU3318 at 3495.118 kb on - strand, within DVU3318 at 3495.146 kb on - strand, within DVU3318 at 3495.182 kb on - strand, within DVU3318 at 3495.230 kb on + strand at 3495.230 kb on + strand at 3495.230 kb on + strand at 3495.238 kb on - strand at 3495.307 kb on - strand at 3495.355 kb on + strand at 3495.396 kb on + strand at 3495.406 kb on + strand at 3495.407 kb on + strand at 3495.407 kb on + strand at 3495.415 kb on - strand at 3495.415 kb on - strand at 3495.417 kb on + strand at 3495.420 kb on - strand at 3495.459 kb on - strand at 3495.472 kb on + strand at 3495.478 kb on + strand at 3495.485 kb on - strand at 3495.494 kb on - strand at 3495.525 kb on + strand at 3495.604 kb on + strand, within putA at 3495.604 kb on + strand, within putA at 3495.607 kb on + strand, within putA at 3495.618 kb on - strand, within putA at 3495.688 kb on - strand, within putA at 3495.797 kb on - strand, within putA
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Uranyl Acetate 1mM remove 3,492,984 - -0.4 3,493,009 + -0.1 3,493,017 - -0.7 3,493,033 + -0.3 3,494,842 + -0.3 3,495,047 - -0.1 3,495,075 - DVU3318 0.26 -0.3 3,495,098 - DVU3318 0.39 +0.1 3,495,117 - DVU3318 0.50 +0.3 3,495,118 - DVU3318 0.51 -0.0 3,495,146 - DVU3318 0.67 +2.2 3,495,182 - DVU3318 0.88 +0.5 3,495,230 + -0.3 3,495,230 + +0.1 3,495,230 + +0.6 3,495,238 - -0.1 3,495,307 - +0.7 3,495,355 + -1.3 3,495,396 + -0.8 3,495,406 + -0.0 3,495,407 + +0.9 3,495,407 + +0.2 3,495,415 - +0.2 3,495,415 - +1.7 3,495,417 + +0.2 3,495,420 - +1.1 3,495,459 - -0.6 3,495,472 + -0.2 3,495,478 + +0.3 3,495,485 - -0.2 3,495,494 - +0.0 3,495,525 + +0.1 3,495,604 + putA DVU3319 0.10 -0.3 3,495,604 + putA DVU3319 0.10 -0.5 3,495,607 + putA DVU3319 0.10 +0.0 3,495,618 - putA DVU3319 0.11 -0.7 3,495,688 - putA DVU3319 0.13 -0.3 3,495,797 - putA DVU3319 0.17 -0.6
Or see this region's nucleotide sequence