Experiment: MoYLS4 with Uranyl Acetate 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheY-3 and fliA are separated by 22 nucleotides fliA and DVU3230 overlap by 47 nucleotides DVU3230 and DVU3231 overlap by 1 nucleotides
DVU3228: cheY-3 - chemotaxis protein CheY (TIGR), at 3,396,417 to 3,396,797
cheY-3
DVU3229: fliA - RNA polymerase sigma factor for flagellar operon FliA (TIGR), at 3,396,820 to 3,397,617
fliA
DVU3230: DVU3230 - flagellar synthesis regulator FleN (TIGR), at 3,397,571 to 3,398,398
DVU3230
DVU3231: DVU3231 - flagellar biosynthesis protein FlhF, putative (TIGR), at 3,398,398 to 3,399,495
DVU3231
Position (kb)
3397
3398
3399 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3396.602 kb on + strand, within cheY-3 at 3396.646 kb on - strand, within cheY-3 at 3396.651 kb on - strand, within cheY-3 at 3396.673 kb on + strand, within cheY-3 at 3396.795 kb on + strand at 3396.797 kb on + strand at 3396.813 kb on - strand at 3396.817 kb on + strand at 3396.825 kb on - strand at 3396.825 kb on - strand at 3396.839 kb on + strand at 3396.876 kb on + strand at 3396.879 kb on + strand at 3397.086 kb on - strand, within fliA at 3397.262 kb on - strand, within fliA at 3397.401 kb on + strand, within fliA at 3397.417 kb on - strand, within fliA at 3397.417 kb on - strand, within fliA at 3397.436 kb on - strand, within fliA at 3397.453 kb on - strand, within fliA at 3397.459 kb on - strand, within fliA at 3397.472 kb on - strand, within fliA at 3397.513 kb on - strand, within fliA at 3397.610 kb on - strand at 3397.669 kb on + strand, within DVU3230 at 3398.159 kb on - strand, within DVU3230 at 3398.363 kb on + strand at 3398.477 kb on - strand at 3398.540 kb on + strand, within DVU3231 at 3398.563 kb on - strand, within DVU3231 at 3398.570 kb on - strand, within DVU3231 at 3398.627 kb on + strand, within DVU3231 at 3398.660 kb on + strand, within DVU3231 at 3398.724 kb on - strand, within DVU3231 at 3398.764 kb on - strand, within DVU3231 at 3398.766 kb on + strand, within DVU3231 at 3398.774 kb on - strand, within DVU3231 at 3398.794 kb on + strand, within DVU3231 at 3398.794 kb on + strand, within DVU3231 at 3398.814 kb on - strand, within DVU3231 at 3398.853 kb on + strand, within DVU3231 at 3398.885 kb on + strand, within DVU3231 at 3398.903 kb on + strand, within DVU3231 at 3398.916 kb on + strand, within DVU3231 at 3398.958 kb on - strand, within DVU3231 at 3398.963 kb on - strand, within DVU3231 at 3399.053 kb on - strand, within DVU3231 at 3399.107 kb on + strand, within DVU3231 at 3399.112 kb on + strand, within DVU3231 at 3399.127 kb on - strand, within DVU3231 at 3399.127 kb on - strand, within DVU3231 at 3399.136 kb on + strand, within DVU3231 at 3399.168 kb on - strand, within DVU3231 at 3399.183 kb on + strand, within DVU3231 at 3399.185 kb on - strand, within DVU3231 at 3399.192 kb on + strand, within DVU3231 at 3399.239 kb on + strand, within DVU3231 at 3399.239 kb on + strand, within DVU3231 at 3399.272 kb on - strand, within DVU3231 at 3399.320 kb on - strand, within DVU3231 at 3399.342 kb on - strand, within DVU3231
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Uranyl Acetate 1mM remove 3,396,602 + cheY-3 DVU3228 0.49 -0.3 3,396,646 - cheY-3 DVU3228 0.60 +0.7 3,396,651 - cheY-3 DVU3228 0.61 +0.1 3,396,673 + cheY-3 DVU3228 0.67 +0.1 3,396,795 + +0.1 3,396,797 + +0.0 3,396,813 - +1.7 3,396,817 + -2.0 3,396,825 - -0.9 3,396,825 - +0.1 3,396,839 + +1.7 3,396,876 + -0.4 3,396,879 + +0.0 3,397,086 - fliA DVU3229 0.33 +0.4 3,397,262 - fliA DVU3229 0.55 +0.5 3,397,401 + fliA DVU3229 0.73 +0.4 3,397,417 - fliA DVU3229 0.75 +1.3 3,397,417 - fliA DVU3229 0.75 +0.4 3,397,436 - fliA DVU3229 0.77 +0.2 3,397,453 - fliA DVU3229 0.79 -0.4 3,397,459 - fliA DVU3229 0.80 -0.8 3,397,472 - fliA DVU3229 0.82 -2.5 3,397,513 - fliA DVU3229 0.87 +0.1 3,397,610 - +1.7 3,397,669 + DVU3230 0.12 +0.7 3,398,159 - DVU3230 0.71 -1.2 3,398,363 + -0.9 3,398,477 - -0.9 3,398,540 + DVU3231 0.13 +0.7 3,398,563 - DVU3231 0.15 +0.1 3,398,570 - DVU3231 0.16 -3.2 3,398,627 + DVU3231 0.21 -0.2 3,398,660 + DVU3231 0.24 +0.4 3,398,724 - DVU3231 0.30 +0.1 3,398,764 - DVU3231 0.33 -0.0 3,398,766 + DVU3231 0.34 -1.4 3,398,774 - DVU3231 0.34 -1.3 3,398,794 + DVU3231 0.36 +0.9 3,398,794 + DVU3231 0.36 -0.5 3,398,814 - DVU3231 0.38 +0.1 3,398,853 + DVU3231 0.41 -0.4 3,398,885 + DVU3231 0.44 -0.3 3,398,903 + DVU3231 0.46 -0.1 3,398,916 + DVU3231 0.47 -0.2 3,398,958 - DVU3231 0.51 -0.0 3,398,963 - DVU3231 0.51 -1.9 3,399,053 - DVU3231 0.60 -0.3 3,399,107 + DVU3231 0.65 -0.3 3,399,112 + DVU3231 0.65 +0.3 3,399,127 - DVU3231 0.66 -0.7 3,399,127 - DVU3231 0.66 +0.1 3,399,136 + DVU3231 0.67 -0.3 3,399,168 - DVU3231 0.70 -1.3 3,399,183 + DVU3231 0.71 -0.2 3,399,185 - DVU3231 0.72 -0.3 3,399,192 + DVU3231 0.72 -0.7 3,399,239 + DVU3231 0.77 -0.5 3,399,239 + DVU3231 0.77 -0.2 3,399,272 - DVU3231 0.80 -0.3 3,399,320 - DVU3231 0.84 +0.7 3,399,342 - DVU3231 0.86 -0.4
Or see this region's nucleotide sequence