Experiment: MoYLS4 with Uranyl Acetate 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argD and dut are separated by 204 nucleotides dut and DVU2349 are separated by 182 nucleotides
DVU2347: argD - acetylornithine aminotransferase (TIGR), at 2,440,531 to 2,441,730
argD
DVU2348: dut - deoxyuridine 5-triphosphate nucleotidohydrolase (TIGR), at 2,441,935 to 2,442,447
dut
DVU2349: DVU2349 - carbohydrate phosphorylase family protein (TIGR), at 2,442,630 to 2,445,197
DVU2349
Position (kb)
2441
2442
2443 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2441.000 kb on + strand, within argD at 2441.008 kb on - strand, within argD at 2441.015 kb on + strand, within argD at 2441.015 kb on + strand, within argD at 2441.023 kb on - strand, within argD at 2441.035 kb on + strand, within argD at 2441.068 kb on - strand, within argD at 2441.092 kb on - strand, within argD at 2441.121 kb on - strand, within argD at 2441.138 kb on + strand, within argD at 2441.151 kb on - strand, within argD at 2441.153 kb on + strand, within argD at 2441.166 kb on - strand, within argD at 2441.288 kb on + strand, within argD at 2441.334 kb on + strand, within argD at 2441.448 kb on - strand, within argD at 2441.473 kb on + strand, within argD at 2441.487 kb on + strand, within argD at 2441.552 kb on + strand, within argD at 2441.560 kb on - strand, within argD at 2441.603 kb on - strand, within argD at 2441.636 kb on + strand at 2441.664 kb on - strand at 2441.701 kb on + strand at 2441.709 kb on - strand at 2441.733 kb on - strand at 2441.745 kb on - strand at 2441.791 kb on + strand at 2441.845 kb on + strand at 2441.911 kb on + strand at 2442.452 kb on - strand at 2442.480 kb on + strand at 2442.628 kb on + strand at 2442.645 kb on + strand at 2442.655 kb on - strand at 2442.665 kb on - strand at 2442.677 kb on + strand at 2442.685 kb on - strand at 2442.685 kb on - strand at 2442.731 kb on - strand at 2442.769 kb on + strand at 2442.810 kb on + strand at 2442.844 kb on - strand at 2442.859 kb on - strand at 2443.095 kb on + strand, within DVU2349 at 2443.099 kb on + strand, within DVU2349 at 2443.263 kb on + strand, within DVU2349 at 2443.271 kb on - strand, within DVU2349 at 2443.271 kb on - strand, within DVU2349 at 2443.351 kb on - strand, within DVU2349
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Uranyl Acetate 1mM remove 2,441,000 + argD DVU2347 0.39 -2.5 2,441,008 - argD DVU2347 0.40 -2.8 2,441,015 + argD DVU2347 0.40 -2.8 2,441,015 + argD DVU2347 0.40 -2.9 2,441,023 - argD DVU2347 0.41 +0.4 2,441,035 + argD DVU2347 0.42 -2.4 2,441,068 - argD DVU2347 0.45 -0.9 2,441,092 - argD DVU2347 0.47 -0.6 2,441,121 - argD DVU2347 0.49 +1.1 2,441,138 + argD DVU2347 0.51 -3.7 2,441,151 - argD DVU2347 0.52 -2.7 2,441,153 + argD DVU2347 0.52 -3.3 2,441,166 - argD DVU2347 0.53 -0.7 2,441,288 + argD DVU2347 0.63 -1.8 2,441,334 + argD DVU2347 0.67 -3.5 2,441,448 - argD DVU2347 0.76 -1.1 2,441,473 + argD DVU2347 0.79 -0.6 2,441,487 + argD DVU2347 0.80 -0.9 2,441,552 + argD DVU2347 0.85 -2.8 2,441,560 - argD DVU2347 0.86 -3.2 2,441,603 - argD DVU2347 0.89 -2.5 2,441,636 + -2.7 2,441,664 - -2.5 2,441,701 + -2.7 2,441,709 - -2.7 2,441,733 - -2.1 2,441,745 - -0.3 2,441,791 + -0.6 2,441,845 + -0.4 2,441,911 + -0.2 2,442,452 - -0.1 2,442,480 + +0.7 2,442,628 + -0.1 2,442,645 + -0.2 2,442,655 - +1.7 2,442,665 - +0.1 2,442,677 + -0.9 2,442,685 - -0.9 2,442,685 - -0.8 2,442,731 - -0.2 2,442,769 + -0.1 2,442,810 + +0.1 2,442,844 - -0.1 2,442,859 - -0.6 2,443,095 + DVU2349 0.18 +0.1 2,443,099 + DVU2349 0.18 -0.4 2,443,263 + DVU2349 0.25 +0.0 2,443,271 - DVU2349 0.25 -0.6 2,443,271 - DVU2349 0.25 -0.4 2,443,351 - DVU2349 0.28 -0.3
Or see this region's nucleotide sequence