Experiment: MoYLS4 with Uranyl Acetate 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2051 and tRNA-Met are separated by 220 nucleotides tRNA-Met and dmt are separated by 102 nucleotides dmt and DVU2053 are separated by 229 nucleotides DVU2053 and DVU2054 overlap by 7 nucleotides
DVU2051: DVU2051 - hypothetical protein (TIGR), at 2,127,643 to 2,128,941
DVU2051
DVU_tRNA-Met-4: tRNA-Met - tRNA with anticodon CAT for Met (VIMSS-AUTO), at 2,129,162 to 2,129,238
tRNA-Met
DVU2052: dmt - glycosyl transferase, group 2 family protein (TIGR), at 2,129,341 to 2,130,063
dmt
DVU2053: DVU2053 - membrane protein, putative (TIGR), at 2,130,293 to 2,130,973
DVU2053
DVU2054: DVU2054 - hypothetical protein (TIGR), at 2,130,967 to 2,131,065
DVU2054
Position (kb)
2129
2130
2131 Strain fitness (log2 ratio)
-1
0
1
2 at 2128.493 kb on + strand, within DVU2051 at 2128.500 kb on + strand, within DVU2051 at 2128.739 kb on + strand, within DVU2051 at 2128.824 kb on - strand at 2129.077 kb on + strand at 2130.052 kb on - strand at 2130.088 kb on - strand at 2130.158 kb on - strand at 2131.021 kb on + strand, within DVU2054 at 2131.063 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Uranyl Acetate 1mM remove 2,128,493 + DVU2051 0.65 -0.2 2,128,500 + DVU2051 0.66 -0.6 2,128,739 + DVU2051 0.84 +0.3 2,128,824 - +0.3 2,129,077 + +2.3 2,130,052 - +1.3 2,130,088 - +0.1 2,130,158 - -1.1 2,131,021 + DVU2054 0.55 +0.1 2,131,063 - +0.7
Or see this region's nucleotide sequence