Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22125

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22120 and BWI76_RS22125 are separated by 66 nucleotidesBWI76_RS22125 and BWI76_RS22130 overlap by 4 nucleotidesBWI76_RS22130 and BWI76_RS22135 are separated by 127 nucleotides BWI76_RS22120: BWI76_RS22120 - glutamate--cysteine ligase, at 4,617,845 to 4,619,401 _RS22120 BWI76_RS22125: BWI76_RS22125 - membrane protein, at 4,619,468 to 4,619,896 _RS22125 BWI76_RS22130: BWI76_RS22130 - fructose-1-phosphate/6-phosphogluconate phosphatase, at 4,619,893 to 4,620,459 _RS22130 BWI76_RS22135: BWI76_RS22135 - transport-associated protein, at 4,620,587 to 4,620,901 _RS22135 Position (kb) 4619 4620Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6 7 8 9 10at 4618.677 kb on + strand, within BWI76_RS22120at 4618.718 kb on - strand, within BWI76_RS22120at 4618.821 kb on - strand, within BWI76_RS22120at 4618.821 kb on - strand, within BWI76_RS22120at 4618.994 kb on + strandat 4619.108 kb on - strand, within BWI76_RS22120at 4619.159 kb on + strand, within BWI76_RS22120at 4619.160 kb on - strand, within BWI76_RS22120at 4619.388 kb on - strandat 4619.594 kb on - strand, within BWI76_RS22125at 4619.772 kb on + strand, within BWI76_RS22125at 4619.780 kb on - strand, within BWI76_RS22125at 4619.847 kb on - strand, within BWI76_RS22125at 4619.881 kb on + strandat 4619.941 kb on + strandat 4620.018 kb on - strand, within BWI76_RS22130at 4620.032 kb on + strand, within BWI76_RS22130at 4620.184 kb on - strand, within BWI76_RS22130at 4620.188 kb on + strand, within BWI76_RS22130at 4620.613 kb on - strandat 4620.718 kb on - strand, within BWI76_RS22135at 4620.762 kb on + strand, within BWI76_RS22135at 4620.796 kb on - strand, within BWI76_RS22135

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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4,618,677 + BWI76_RS22120 0.53 +10.2
4,618,718 - BWI76_RS22120 0.56 +9.8
4,618,821 - BWI76_RS22120 0.63 +10.1
4,618,821 - BWI76_RS22120 0.63 +10.2
4,618,994 + +10.1
4,619,108 - BWI76_RS22120 0.81 +10.2
4,619,159 + BWI76_RS22120 0.84 +9.6
4,619,160 - BWI76_RS22120 0.84 +10.3
4,619,388 - +10.1
4,619,594 - BWI76_RS22125 0.29 -0.3
4,619,772 + BWI76_RS22125 0.71 +1.1
4,619,780 - BWI76_RS22125 0.73 +0.0
4,619,847 - BWI76_RS22125 0.88 -2.8
4,619,881 + +1.8
4,619,941 + +2.0
4,620,018 - BWI76_RS22130 0.22 -0.5
4,620,032 + BWI76_RS22130 0.25 +2.1
4,620,184 - BWI76_RS22130 0.51 -0.1
4,620,188 + BWI76_RS22130 0.52 +4.0
4,620,613 - +0.7
4,620,718 - BWI76_RS22135 0.42 +1.0
4,620,762 + BWI76_RS22135 0.56 +1.0
4,620,796 - BWI76_RS22135 0.66 -0.8

Or see this region's nucleotide sequence