Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02615

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02605 and BWI76_RS02610 overlap by 4 nucleotidesBWI76_RS02610 and BWI76_RS02615 are separated by 106 nucleotidesBWI76_RS02615 and BWI76_RS02620 are separated by 364 nucleotides BWI76_RS02605: BWI76_RS02605 - esterase, at 533,053 to 533,769 _RS02605 BWI76_RS02610: BWI76_RS02610 - transcriptional regulator, at 533,766 to 534,521 _RS02610 BWI76_RS02615: BWI76_RS02615 - L-ascorbate-6-phosphate lactonase, at 534,628 to 535,692 _RS02615 BWI76_RS02620: BWI76_RS02620 - PTS ascorbate transporter subunit IIC, at 536,057 to 537,457 _RS02620 Position (kb) 534 535 536Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 533.794 kb on + strandat 533.866 kb on + strand, within BWI76_RS02610at 533.902 kb on + strand, within BWI76_RS02610at 533.974 kb on + strand, within BWI76_RS02610at 533.982 kb on - strand, within BWI76_RS02610at 534.002 kb on - strand, within BWI76_RS02610at 534.053 kb on + strand, within BWI76_RS02610at 534.073 kb on - strand, within BWI76_RS02610at 534.082 kb on + strand, within BWI76_RS02610at 534.165 kb on + strand, within BWI76_RS02610at 534.633 kb on - strandat 534.646 kb on - strandat 534.646 kb on - strandat 534.652 kb on + strandat 534.659 kb on - strandat 534.905 kb on - strand, within BWI76_RS02615at 535.039 kb on - strand, within BWI76_RS02615at 535.131 kb on - strand, within BWI76_RS02615at 535.134 kb on - strand, within BWI76_RS02615at 535.134 kb on - strand, within BWI76_RS02615at 535.156 kb on - strand, within BWI76_RS02615at 535.240 kb on + strand, within BWI76_RS02615at 535.241 kb on - strand, within BWI76_RS02615at 535.291 kb on + strand, within BWI76_RS02615at 535.299 kb on - strandat 535.415 kb on + strand, within BWI76_RS02615at 535.527 kb on - strand, within BWI76_RS02615at 535.602 kb on - strandat 535.799 kb on + strandat 536.082 kb on - strandat 536.098 kb on + strandat 536.106 kb on - strandat 536.145 kb on + strandat 536.153 kb on - strandat 536.216 kb on - strand, within BWI76_RS02620at 536.216 kb on - strand, within BWI76_RS02620at 536.245 kb on - strand, within BWI76_RS02620at 536.275 kb on + strand, within BWI76_RS02620at 536.283 kb on - strand, within BWI76_RS02620at 536.344 kb on + strand, within BWI76_RS02620at 536.354 kb on + strand, within BWI76_RS02620at 536.368 kb on + strandat 536.370 kb on + strand, within BWI76_RS02620at 536.376 kb on - strand, within BWI76_RS02620at 536.389 kb on - strand, within BWI76_RS02620at 536.406 kb on - strand, within BWI76_RS02620at 536.533 kb on + strand, within BWI76_RS02620at 536.568 kb on - strand, within BWI76_RS02620

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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533,794 + +0.4
533,866 + BWI76_RS02610 0.13 +0.5
533,902 + BWI76_RS02610 0.18 -1.7
533,974 + BWI76_RS02610 0.28 +0.7
533,982 - BWI76_RS02610 0.29 -0.1
534,002 - BWI76_RS02610 0.31 +0.5
534,053 + BWI76_RS02610 0.38 +0.9
534,073 - BWI76_RS02610 0.41 -0.5
534,082 + BWI76_RS02610 0.42 -1.1
534,165 + BWI76_RS02610 0.53 -0.8
534,633 - +0.2
534,646 - +1.5
534,646 - +0.1
534,652 + -1.0
534,659 - -1.3
534,905 - BWI76_RS02615 0.26 -0.5
535,039 - BWI76_RS02615 0.39 -0.5
535,131 - BWI76_RS02615 0.47 -0.6
535,134 - BWI76_RS02615 0.48 +1.6
535,134 - BWI76_RS02615 0.48 -0.8
535,156 - BWI76_RS02615 0.50 -0.6
535,240 + BWI76_RS02615 0.57 -0.0
535,241 - BWI76_RS02615 0.58 -1.0
535,291 + BWI76_RS02615 0.62 -1.0
535,299 - +0.6
535,415 + BWI76_RS02615 0.74 -0.4
535,527 - BWI76_RS02615 0.84 -0.1
535,602 - -1.8
535,799 + +0.7
536,082 - -0.8
536,098 + +0.1
536,106 - -0.1
536,145 + +1.2
536,153 - -0.8
536,216 - BWI76_RS02620 0.11 +1.5
536,216 - BWI76_RS02620 0.11 -0.1
536,245 - BWI76_RS02620 0.13 +1.2
536,275 + BWI76_RS02620 0.16 -1.3
536,283 - BWI76_RS02620 0.16 +2.2
536,344 + BWI76_RS02620 0.20 -0.1
536,354 + BWI76_RS02620 0.21 +0.4
536,368 + +0.6
536,370 + BWI76_RS02620 0.22 +0.7
536,376 - BWI76_RS02620 0.23 +3.1
536,389 - BWI76_RS02620 0.24 +3.2
536,406 - BWI76_RS02620 0.25 +0.2
536,533 + BWI76_RS02620 0.34 +1.7
536,568 - BWI76_RS02620 0.36 +4.2

Or see this region's nucleotide sequence