Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00080

Experiment: Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00070 and BWI76_RS00075 are separated by 50 nucleotidesBWI76_RS00075 and BWI76_RS00080 overlap by 4 nucleotidesBWI76_RS00080 and BWI76_RS00085 overlap by 4 nucleotidesBWI76_RS00085 and BWI76_RS00090 are separated by 169 nucleotides BWI76_RS00070: BWI76_RS00070 - MFS transporter, at 13,479 to 14,969 _RS00070 BWI76_RS00075: BWI76_RS00075 - N-acetylmannosamine-6-phosphate 2-epimerase, at 15,020 to 15,709 _RS00075 BWI76_RS00080: BWI76_RS00080 - N-acetylmannosamine kinase, at 15,706 to 16,581 _RS00080 BWI76_RS00085: BWI76_RS00085 - hypothetical protein, at 16,578 to 17,042 _RS00085 BWI76_RS00090: BWI76_RS00090 - multidrug transporter subunit MdtL, at 17,212 to 18,393 _RS00090 Position (kb) 15 16 17Strain fitness (log2 ratio) -1 0 1 2 3at 14.759 kb on + strand, within BWI76_RS00070at 14.884 kb on + strandat 14.922 kb on - strandat 14.925 kb on - strandat 14.925 kb on - strandat 15.018 kb on - strandat 15.089 kb on + strand, within BWI76_RS00075at 15.320 kb on - strand, within BWI76_RS00075at 15.361 kb on + strand, within BWI76_RS00075at 16.009 kb on + strand, within BWI76_RS00080at 16.225 kb on + strand, within BWI76_RS00080at 16.451 kb on + strand, within BWI76_RS00080at 16.461 kb on + strand, within BWI76_RS00080at 16.469 kb on - strand, within BWI76_RS00080at 16.514 kb on - strandat 16.529 kb on - strandat 16.601 kb on - strandat 16.609 kb on + strandat 16.616 kb on + strandat 16.631 kb on + strand, within BWI76_RS00085at 16.631 kb on + strand, within BWI76_RS00085at 16.932 kb on - strand, within BWI76_RS00085at 17.020 kb on - strandat 17.088 kb on - strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.184 kb on - strandat 17.184 kb on - strandat 17.214 kb on - strandat 17.244 kb on + strandat 17.252 kb on - strandat 17.252 kb on - strandat 17.498 kb on - strand, within BWI76_RS00090at 17.539 kb on + strand, within BWI76_RS00090at 17.546 kb on + strand, within BWI76_RS00090

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Per-strain Table

Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM
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14,759 + BWI76_RS00070 0.86 -0.1
14,884 + +1.2
14,922 - +2.8
14,925 - -0.9
14,925 - -1.0
15,018 - +2.2
15,089 + BWI76_RS00075 0.10 -1.7
15,320 - BWI76_RS00075 0.43 -0.4
15,361 + BWI76_RS00075 0.49 +0.7
16,009 + BWI76_RS00080 0.35 -1.4
16,225 + BWI76_RS00080 0.59 -1.4
16,451 + BWI76_RS00080 0.85 -1.2
16,461 + BWI76_RS00080 0.86 +1.2
16,469 - BWI76_RS00080 0.87 -0.6
16,514 - +1.6
16,529 - +2.0
16,601 - +0.8
16,609 + +1.8
16,616 + -1.6
16,631 + BWI76_RS00085 0.11 -0.3
16,631 + BWI76_RS00085 0.11 +0.4
16,932 - BWI76_RS00085 0.76 +0.1
17,020 - -0.2
17,088 - +2.2
17,176 + -0.1
17,176 + -0.6
17,176 + +0.0
17,176 + -0.4
17,184 - -0.7
17,184 - -0.0
17,214 - -0.4
17,244 + -0.1
17,252 - -0.1
17,252 - -0.5
17,498 - BWI76_RS00090 0.24 -0.5
17,539 + BWI76_RS00090 0.28 -0.2
17,546 + BWI76_RS00090 0.28 +0.6

Or see this region's nucleotide sequence