Experiment: Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4650 and BT4651 are separated by 16 nucleotides BT4651 and BT4652 are separated by 359 nucleotides
BT4650: BT4650 - voltage-gated chloride channel protein (NCBI ptt file), at 6,083,076 to 6,084,650
BT4650
BT4651: BT4651 - endonuclease IV (NCBI ptt file), at 6,084,667 to 6,085,500
BT4651
BT4652: BT4652 - heparan sulfate cleaving enzyme (from data) , at 6,085,860 to 6,088,478
BT4652
Position (kb)
6084
6085
6086 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6083.685 kb on - strand at 6083.881 kb on - strand, within BT4650 at 6083.909 kb on - strand, within BT4650 at 6083.919 kb on + strand, within BT4650 at 6083.919 kb on + strand, within BT4650 at 6083.919 kb on + strand, within BT4650 at 6083.920 kb on - strand, within BT4650 at 6083.931 kb on - strand, within BT4650 at 6084.143 kb on - strand, within BT4650 at 6084.188 kb on + strand, within BT4650 at 6084.198 kb on - strand, within BT4650 at 6084.198 kb on - strand, within BT4650 at 6084.198 kb on - strand, within BT4650 at 6084.198 kb on - strand, within BT4650 at 6084.212 kb on + strand, within BT4650 at 6084.213 kb on - strand, within BT4650 at 6084.243 kb on - strand, within BT4650 at 6084.243 kb on - strand, within BT4650 at 6084.309 kb on - strand, within BT4650 at 6084.333 kb on + strand, within BT4650 at 6084.355 kb on + strand, within BT4650 at 6084.355 kb on + strand, within BT4650 at 6084.376 kb on + strand, within BT4650 at 6084.387 kb on - strand, within BT4650 at 6084.423 kb on + strand, within BT4650 at 6084.424 kb on - strand, within BT4650 at 6084.437 kb on - strand, within BT4650 at 6084.503 kb on + strand at 6084.506 kb on + strand at 6084.512 kb on + strand at 6084.513 kb on - strand at 6084.632 kb on + strand at 6084.641 kb on + strand at 6084.656 kb on - strand at 6084.931 kb on + strand, within BT4651 at 6084.932 kb on - strand, within BT4651 at 6084.948 kb on + strand, within BT4651 at 6084.950 kb on + strand, within BT4651 at 6084.950 kb on + strand, within BT4651 at 6084.951 kb on - strand, within BT4651 at 6084.951 kb on - strand, within BT4651 at 6084.955 kb on - strand, within BT4651 at 6084.976 kb on - strand, within BT4651 at 6085.011 kb on + strand, within BT4651 at 6085.011 kb on + strand, within BT4651 at 6085.018 kb on + strand, within BT4651 at 6085.018 kb on + strand, within BT4651 at 6085.019 kb on - strand, within BT4651 at 6085.090 kb on - strand, within BT4651 at 6085.090 kb on - strand, within BT4651 at 6085.281 kb on + strand, within BT4651 at 6085.282 kb on - strand, within BT4651 at 6085.305 kb on + strand, within BT4651 at 6085.329 kb on + strand, within BT4651 at 6085.478 kb on - strand at 6085.478 kb on - strand at 6085.521 kb on + strand at 6085.534 kb on - strand at 6085.540 kb on + strand at 6085.540 kb on + strand at 6085.541 kb on - strand at 6085.567 kb on + strand at 6085.622 kb on + strand at 6085.638 kb on + strand at 6085.649 kb on + strand at 6085.668 kb on - strand at 6085.692 kb on + strand at 6085.718 kb on - strand at 6085.718 kb on - strand at 6085.734 kb on - strand at 6085.736 kb on + strand at 6085.756 kb on - strand at 6085.758 kb on + strand at 6085.759 kb on - strand at 6085.759 kb on - strand at 6085.778 kb on - strand at 6085.802 kb on - strand at 6085.808 kb on - strand at 6085.814 kb on - strand at 6085.815 kb on + strand at 6085.837 kb on + strand at 6085.882 kb on + strand at 6086.040 kb on - strand at 6086.053 kb on - strand at 6086.053 kb on - strand at 6086.053 kb on - strand at 6086.058 kb on + strand at 6086.058 kb on + strand at 6086.058 kb on + strand at 6086.059 kb on - strand at 6086.059 kb on - strand at 6086.100 kb on - strand at 6086.117 kb on + strand at 6086.212 kb on - strand, within BT4652 at 6086.250 kb on + strand, within BT4652 at 6086.250 kb on + strand, within BT4652 at 6086.251 kb on - strand, within BT4652 at 6086.376 kb on - strand, within BT4652 at 6086.387 kb on - strand, within BT4652
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.2 mM remove 6,083,685 - +0.0 6,083,881 - BT4650 0.51 -2.3 6,083,909 - BT4650 0.53 -3.1 6,083,919 + BT4650 0.54 -0.5 6,083,919 + BT4650 0.54 -1.6 6,083,919 + BT4650 0.54 -1.4 6,083,920 - BT4650 0.54 -1.6 6,083,931 - BT4650 0.54 -1.0 6,084,143 - BT4650 0.68 -1.0 6,084,188 + BT4650 0.71 -0.2 6,084,198 - BT4650 0.71 -1.0 6,084,198 - BT4650 0.71 +0.2 6,084,198 - BT4650 0.71 -1.6 6,084,198 - BT4650 0.71 -3.4 6,084,212 + BT4650 0.72 -1.6 6,084,213 - BT4650 0.72 +0.4 6,084,243 - BT4650 0.74 +0.6 6,084,243 - BT4650 0.74 -2.3 6,084,309 - BT4650 0.78 -1.6 6,084,333 + BT4650 0.80 -2.3 6,084,355 + BT4650 0.81 -0.2 6,084,355 + BT4650 0.81 -0.6 6,084,376 + BT4650 0.83 +0.0 6,084,387 - BT4650 0.83 -0.4 6,084,423 + BT4650 0.86 -0.6 6,084,424 - BT4650 0.86 -1.4 6,084,437 - BT4650 0.86 -1.3 6,084,503 + +0.0 6,084,506 + -1.6 6,084,512 + -1.0 6,084,513 - -1.0 6,084,632 + -2.1 6,084,641 + -0.8 6,084,656 - -2.3 6,084,931 + BT4651 0.32 -3.0 6,084,932 - BT4651 0.32 -1.1 6,084,948 + BT4651 0.34 -1.6 6,084,950 + BT4651 0.34 -2.1 6,084,950 + BT4651 0.34 -0.7 6,084,951 - BT4651 0.34 -1.6 6,084,951 - BT4651 0.34 -1.6 6,084,955 - BT4651 0.35 -2.0 6,084,976 - BT4651 0.37 +2.0 6,085,011 + BT4651 0.41 -2.0 6,085,011 + BT4651 0.41 +0.0 6,085,018 + BT4651 0.42 -0.7 6,085,018 + BT4651 0.42 -1.0 6,085,019 - BT4651 0.42 -3.0 6,085,090 - BT4651 0.51 -1.0 6,085,090 - BT4651 0.51 -2.1 6,085,281 + BT4651 0.74 -2.8 6,085,282 - BT4651 0.74 -2.6 6,085,305 + BT4651 0.76 -1.7 6,085,329 + BT4651 0.79 -1.7 6,085,478 - -1.0 6,085,478 - +1.0 6,085,521 + -0.7 6,085,534 - -2.6 6,085,540 + -1.3 6,085,540 + -1.0 6,085,541 - -1.4 6,085,567 + +0.0 6,085,622 + -3.0 6,085,638 + +0.6 6,085,649 + +0.0 6,085,668 - +0.0 6,085,692 + -2.3 6,085,718 - -2.0 6,085,718 - +0.8 6,085,734 - -1.6 6,085,736 + -0.6 6,085,756 - -1.6 6,085,758 + -0.5 6,085,759 - -0.6 6,085,759 - +1.0 6,085,778 - -1.0 6,085,802 - +1.3 6,085,808 - -1.0 6,085,814 - +0.0 6,085,815 + -0.3 6,085,837 + +1.0 6,085,882 + -3.0 6,086,040 - +0.4 6,086,053 - +0.0 6,086,053 - +0.0 6,086,053 - +1.6 6,086,058 + +2.8 6,086,058 + +0.0 6,086,058 + +0.0 6,086,059 - -1.4 6,086,059 - +2.0 6,086,100 - -1.0 6,086,117 + -2.7 6,086,212 - BT4652 0.13 -0.6 6,086,250 + BT4652 0.15 -2.3 6,086,250 + BT4652 0.15 -1.6 6,086,251 - BT4652 0.15 -1.8 6,086,376 - BT4652 0.20 -1.6 6,086,387 - BT4652 0.20 +2.0
Or see this region's nucleotide sequence