Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4442

Experiment: Varel_Bryant_medium_Glucose with Phenazine ethosulfate 0.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4440 and BT4441 are separated by 16 nucleotidesBT4441 and BT4442 are separated by 227 nucleotidesBT4442 and BT4443 overlap by 47 nucleotidesBT4443 and BT4444 overlap by 4 nucleotides BT4440: BT4440 - conserved hypothetical protein, putative cell surface protein (NCBI ptt file), at 5,846,629 to 5,853,180 BT4440 BT4441: BT4441 - cell surface protein (NCBI ptt file), at 5,853,197 to 5,853,922 BT4441 BT4442: BT4442 - hypothetical protein (NCBI ptt file), at 5,854,150 to 5,854,500 BT4442 BT4443: BT4443 - N-acetylmuramoyl alanine amidase (NCBI ptt file), at 5,854,454 to 5,855,110 BT4443 BT4444: BT4444 - hypothetical protein (NCBI ptt file), at 5,855,107 to 5,855,505 BT4444 Position (kb) 5854 5855Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 5853.204 kb on - strandat 5853.305 kb on + strand, within BT4441at 5853.380 kb on - strand, within BT4441at 5853.444 kb on - strand, within BT4441at 5853.464 kb on + strand, within BT4441at 5853.494 kb on + strandat 5853.495 kb on - strand, within BT4441at 5853.521 kb on - strand, within BT4441at 5853.521 kb on - strand, within BT4441at 5853.521 kb on - strand, within BT4441at 5853.745 kb on - strand, within BT4441at 5853.808 kb on - strand, within BT4441at 5853.864 kb on - strandat 5854.015 kb on - strandat 5854.050 kb on - strandat 5854.095 kb on - strandat 5854.170 kb on - strandat 5854.225 kb on + strand, within BT4442at 5854.324 kb on - strand, within BT4442at 5854.353 kb on + strand, within BT4442at 5854.353 kb on + strand, within BT4442at 5854.353 kb on + strand, within BT4442at 5854.424 kb on + strand, within BT4442at 5854.534 kb on - strand, within BT4443at 5854.534 kb on - strand, within BT4443at 5854.537 kb on + strand, within BT4443at 5854.604 kb on - strand, within BT4443at 5854.666 kb on + strand, within BT4443at 5854.690 kb on - strand, within BT4443at 5854.690 kb on - strand, within BT4443at 5854.690 kb on - strand, within BT4443at 5854.690 kb on - strand, within BT4443at 5854.701 kb on + strand, within BT4443at 5854.701 kb on + strand, within BT4443at 5854.789 kb on + strandat 5854.800 kb on + strand, within BT4443at 5854.852 kb on + strand, within BT4443at 5854.853 kb on - strand, within BT4443at 5854.953 kb on + strand, within BT4443at 5854.963 kb on + strand, within BT4443at 5855.069 kb on - strandat 5855.084 kb on - strandat 5855.117 kb on + strandat 5855.118 kb on - strandat 5855.125 kb on - strandat 5855.125 kb on - strandat 5855.125 kb on - strandat 5855.126 kb on + strandat 5855.127 kb on - strandat 5855.180 kb on - strand, within BT4444at 5855.226 kb on + strand, within BT4444at 5855.227 kb on - strand, within BT4444at 5855.228 kb on + strand, within BT4444at 5855.244 kb on - strand, within BT4444at 5855.257 kb on - strand, within BT4444at 5855.264 kb on - strand, within BT4444at 5855.284 kb on - strand, within BT4444at 5855.291 kb on - strand, within BT4444at 5855.308 kb on + strand, within BT4444at 5855.315 kb on - strand, within BT4444at 5855.320 kb on + strand, within BT4444at 5855.448 kb on + strand, within BT4444at 5855.452 kb on - strand, within BT4444at 5855.491 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Phenazine ethosulfate 0.2 mM
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5,853,204 - -0.1
5,853,305 + BT4441 0.15 -2.4
5,853,380 - BT4441 0.25 -0.1
5,853,444 - BT4441 0.34 -2.4
5,853,464 + BT4441 0.37 -2.1
5,853,494 + -1.1
5,853,495 - BT4441 0.41 -4.0
5,853,521 - BT4441 0.45 -3.7
5,853,521 - BT4441 0.45 -2.1
5,853,521 - BT4441 0.45 +0.5
5,853,745 - BT4441 0.75 -1.1
5,853,808 - BT4441 0.84 -0.7
5,853,864 - -2.8
5,854,015 - -3.1
5,854,050 - -1.6
5,854,095 - -0.8
5,854,170 - -0.1
5,854,225 + BT4442 0.21 +0.8
5,854,324 - BT4442 0.50 +2.3
5,854,353 + BT4442 0.58 +0.9
5,854,353 + BT4442 0.58 +0.3
5,854,353 + BT4442 0.58 -2.4
5,854,424 + BT4442 0.78 -0.1
5,854,534 - BT4443 0.12 -2.9
5,854,534 - BT4443 0.12 -1.9
5,854,537 + BT4443 0.13 +1.8
5,854,604 - BT4443 0.23 -1.6
5,854,666 + BT4443 0.32 -1.1
5,854,690 - BT4443 0.36 -1.6
5,854,690 - BT4443 0.36 -1.1
5,854,690 - BT4443 0.36 -0.1
5,854,690 - BT4443 0.36 -0.1
5,854,701 + BT4443 0.38 -1.7
5,854,701 + BT4443 0.38 -0.1
5,854,789 + -1.6
5,854,800 + BT4443 0.53 -3.5
5,854,852 + BT4443 0.61 +0.2
5,854,853 - BT4443 0.61 -3.1
5,854,953 + BT4443 0.76 -0.1
5,854,963 + BT4443 0.77 +0.9
5,855,069 - -2.7
5,855,084 - -1.7
5,855,117 + -3.4
5,855,118 - -1.1
5,855,125 - -1.5
5,855,125 - -1.9
5,855,125 - -0.3
5,855,126 + -0.1
5,855,127 - +1.5
5,855,180 - BT4444 0.18 -1.7
5,855,226 + BT4444 0.30 -2.9
5,855,227 - BT4444 0.30 -2.7
5,855,228 + BT4444 0.30 -2.4
5,855,244 - BT4444 0.34 -0.1
5,855,257 - BT4444 0.38 +0.4
5,855,264 - BT4444 0.39 +1.3
5,855,284 - BT4444 0.44 -1.7
5,855,291 - BT4444 0.46 -0.1
5,855,308 + BT4444 0.50 -3.2
5,855,315 - BT4444 0.52 +0.6
5,855,320 + BT4444 0.53 -1.0
5,855,448 + BT4444 0.85 -0.1
5,855,452 - BT4444 0.86 -0.2
5,855,491 - -3.2

Or see this region's nucleotide sequence