Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT0466

Experiment: Varel_Bryant_medium_Glucose with Phenazine ethosulfate 0.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT0464 and BT0465 are separated by 3 nucleotidesBT0465 and BT0466 are separated by 2 nucleotidesBT0466 and BT0467 are separated by 202 nucleotides BT0464: BT0464 - dTDP-4-dehydrorhamnose 3,5-epimerase (NCBI ptt file), at 574,814 to 575,383 BT0464 BT0465: BT0465 - dTDP-4-dehydrorhamnose reductase (NCBI ptt file), at 575,387 to 576,241 BT0465 BT0466: BT0466 - dTDP-glucose 4,6-dehydratase (NCBI ptt file), at 576,244 to 577,356 BT0466 BT0467: BT0467 - conserved hypothetical protein (NCBI ptt file), at 577,559 to 579,028 BT0467 Position (kb) 576 577 578Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 575.376 kb on + strandat 575.410 kb on + strandat 575.461 kb on + strandat 575.470 kb on - strandat 575.527 kb on - strand, within BT0465at 575.547 kb on + strand, within BT0465at 575.582 kb on - strand, within BT0465at 575.624 kb on - strand, within BT0465at 575.680 kb on + strand, within BT0465at 575.710 kb on + strand, within BT0465at 575.716 kb on + strand, within BT0465at 575.744 kb on - strand, within BT0465at 575.840 kb on + strand, within BT0465at 575.894 kb on - strand, within BT0465at 575.936 kb on - strand, within BT0465at 576.005 kb on - strand, within BT0465at 576.005 kb on - strand, within BT0465at 576.005 kb on - strand, within BT0465at 576.008 kb on + strand, within BT0465at 576.148 kb on - strand, within BT0465at 576.155 kb on - strand, within BT0465at 576.340 kb on - strandat 576.409 kb on - strand, within BT0466at 576.409 kb on - strand, within BT0466at 576.409 kb on - strand, within BT0466at 576.422 kb on + strand, within BT0466at 576.475 kb on - strand, within BT0466at 576.525 kb on + strand, within BT0466at 576.652 kb on - strand, within BT0466at 576.754 kb on - strand, within BT0466at 576.754 kb on - strand, within BT0466at 576.789 kb on + strand, within BT0466at 576.891 kb on + strand, within BT0466at 576.974 kb on - strand, within BT0466at 576.974 kb on - strand, within BT0466at 576.978 kb on - strand, within BT0466at 577.039 kb on - strand, within BT0466at 577.042 kb on - strand, within BT0466at 577.110 kb on - strand, within BT0466at 577.113 kb on - strand, within BT0466at 577.147 kb on - strand, within BT0466at 577.179 kb on - strand, within BT0466at 577.278 kb on - strandat 577.278 kb on - strandat 577.343 kb on - strandat 577.442 kb on - strandat 577.494 kb on - strandat 577.517 kb on - strandat 577.537 kb on + strandat 577.578 kb on + strandat 577.579 kb on - strandat 577.676 kb on - strandat 577.678 kb on - strandat 577.774 kb on - strand, within BT0467at 577.778 kb on - strand, within BT0467at 577.825 kb on - strand, within BT0467at 577.855 kb on + strand, within BT0467at 577.943 kb on - strand, within BT0467at 577.955 kb on - strand, within BT0467at 577.955 kb on - strand, within BT0467at 578.041 kb on - strand, within BT0467at 578.112 kb on - strand, within BT0467

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Phenazine ethosulfate 0.2 mM
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575,376 + +1.6
575,410 + -1.6
575,461 + -1.6
575,470 - -2.3
575,527 - BT0465 0.16 -2.6
575,547 + BT0465 0.19 -3.3
575,582 - BT0465 0.23 -1.0
575,624 - BT0465 0.28 -1.8
575,680 + BT0465 0.34 -2.8
575,710 + BT0465 0.38 -1.0
575,716 + BT0465 0.38 -2.6
575,744 - BT0465 0.42 +0.0
575,840 + BT0465 0.53 -1.0
575,894 - BT0465 0.59 -1.0
575,936 - BT0465 0.64 +0.6
576,005 - BT0465 0.72 +1.2
576,005 - BT0465 0.72 -1.0
576,005 - BT0465 0.72 -2.3
576,008 + BT0465 0.73 +1.0
576,148 - BT0465 0.89 -1.0
576,155 - BT0465 0.90 -4.1
576,340 - -0.7
576,409 - BT0466 0.15 +0.0
576,409 - BT0466 0.15 +1.3
576,409 - BT0466 0.15 -1.6
576,422 + BT0466 0.16 +0.7
576,475 - BT0466 0.21 -2.3
576,525 + BT0466 0.25 -2.6
576,652 - BT0466 0.37 -1.6
576,754 - BT0466 0.46 +0.0
576,754 - BT0466 0.46 -1.8
576,789 + BT0466 0.49 -2.3
576,891 + BT0466 0.58 -0.6
576,974 - BT0466 0.66 +0.7
576,974 - BT0466 0.66 -2.8
576,978 - BT0466 0.66 +2.3
577,039 - BT0466 0.71 +0.0
577,042 - BT0466 0.72 -0.4
577,110 - BT0466 0.78 -1.4
577,113 - BT0466 0.78 +0.0
577,147 - BT0466 0.81 -2.3
577,179 - BT0466 0.84 +0.3
577,278 - +0.0
577,278 - -1.0
577,343 - +0.0
577,442 - +0.0
577,494 - -0.5
577,517 - +0.0
577,537 + +0.0
577,578 + -2.3
577,579 - -0.6
577,676 - -0.4
577,678 - -2.8
577,774 - BT0467 0.15 +3.6
577,778 - BT0467 0.15 -2.3
577,825 - BT0467 0.18 -3.3
577,855 + BT0467 0.20 -1.3
577,943 - BT0467 0.26 -2.8
577,955 - BT0467 0.27 +0.0
577,955 - BT0467 0.27 +1.1
578,041 - BT0467 0.33 +0.0
578,112 - BT0467 0.38 -2.3

Or see this region's nucleotide sequence