Experiment: Mixed culture; Prochlorococcus marinus str. MED4 10:1 starting OD to HP15
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1044 and HP15_1045 are separated by 71 nucleotides HP15_1045 and HP15_1047 are separated by 114 nucleotides HP15_1047 and HP15_1046 are separated by 81 nucleotides HP15_1046 and HP15_1048 are separated by 164 nucleotides
HP15_1044: HP15_1044 - lysine exporter protein (LYSE/YGGA), at 1,065,220 to 1,065,858
_1044
HP15_1045: HP15_1045 - 4Fe-4S ferredoxin, iron-sulfur binding domain protein, at 1,065,930 to 1,066,289
_1045
HP15_1047: HP15_1047 - DNA mismatch repair protein MutS, at 1,066,404 to 1,069,034
_1047
HP15_1046: HP15_1046 - competence damage-inducible protein A, at 1,069,116 to 1,069,616
_1046
HP15_1048: HP15_1048 - recombinase A, at 1,069,781 to 1,070,827
_1048
Position (kb)
1066
1067
1068
1069
1070 Strain fitness (log2 ratio)
-1
0
1
2 at 1065.405 kb on - strand, within HP15_1044 at 1065.408 kb on + strand, within HP15_1044 at 1065.627 kb on + strand, within HP15_1044 at 1065.637 kb on + strand, within HP15_1044 at 1065.674 kb on + strand, within HP15_1044 at 1065.690 kb on + strand, within HP15_1044 at 1065.723 kb on + strand, within HP15_1044 at 1065.724 kb on - strand, within HP15_1044 at 1065.749 kb on - strand, within HP15_1044 at 1065.846 kb on + strand at 1066.331 kb on + strand at 1066.372 kb on + strand at 1066.372 kb on + strand at 1066.391 kb on + strand at 1066.399 kb on - strand at 1066.427 kb on - strand at 1066.509 kb on - strand at 1066.516 kb on - strand at 1066.582 kb on - strand at 1066.599 kb on + strand at 1066.635 kb on - strand at 1066.641 kb on - strand at 1066.711 kb on + strand, within HP15_1047 at 1066.719 kb on - strand, within HP15_1047 at 1066.856 kb on + strand, within HP15_1047 at 1066.886 kb on - strand, within HP15_1047 at 1066.892 kb on - strand, within HP15_1047 at 1066.897 kb on + strand, within HP15_1047 at 1066.924 kb on - strand, within HP15_1047 at 1066.937 kb on + strand, within HP15_1047 at 1066.955 kb on + strand, within HP15_1047 at 1066.980 kb on + strand, within HP15_1047 at 1067.127 kb on - strand, within HP15_1047 at 1067.188 kb on + strand, within HP15_1047 at 1067.234 kb on + strand, within HP15_1047 at 1067.241 kb on + strand, within HP15_1047 at 1067.242 kb on + strand, within HP15_1047 at 1067.278 kb on - strand, within HP15_1047 at 1067.320 kb on - strand, within HP15_1047 at 1067.343 kb on - strand, within HP15_1047 at 1067.442 kb on + strand, within HP15_1047 at 1067.460 kb on + strand at 1067.475 kb on - strand, within HP15_1047 at 1067.475 kb on - strand, within HP15_1047 at 1067.769 kb on + strand, within HP15_1047 at 1067.769 kb on + strand, within HP15_1047 at 1067.769 kb on + strand, within HP15_1047 at 1067.769 kb on + strand, within HP15_1047 at 1067.777 kb on - strand, within HP15_1047 at 1067.894 kb on + strand, within HP15_1047 at 1067.959 kb on - strand, within HP15_1047 at 1068.033 kb on + strand, within HP15_1047 at 1068.054 kb on - strand, within HP15_1047 at 1068.144 kb on + strand, within HP15_1047 at 1068.162 kb on + strand, within HP15_1047 at 1068.164 kb on - strand, within HP15_1047 at 1068.164 kb on - strand, within HP15_1047 at 1068.248 kb on + strand, within HP15_1047 at 1068.248 kb on + strand, within HP15_1047 at 1068.606 kb on - strand, within HP15_1047 at 1068.608 kb on + strand, within HP15_1047 at 1068.616 kb on - strand, within HP15_1047 at 1068.723 kb on - strand, within HP15_1047 at 1068.793 kb on + strand at 1068.877 kb on - strand at 1068.877 kb on - strand at 1068.897 kb on + strand at 1068.907 kb on + strand at 1068.915 kb on - strand at 1069.038 kb on - strand at 1069.118 kb on + strand at 1069.394 kb on + strand, within HP15_1046 at 1069.432 kb on - strand, within HP15_1046 at 1069.488 kb on + strand, within HP15_1046
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Prochlorococcus marinus str. MED4 10:1 starting OD to HP15 remove 1,065,405 - HP15_1044 0.29 +0.6 1,065,408 + HP15_1044 0.29 +0.5 1,065,627 + HP15_1044 0.64 +0.7 1,065,637 + HP15_1044 0.65 +0.8 1,065,674 + HP15_1044 0.71 -0.8 1,065,690 + HP15_1044 0.74 +0.3 1,065,723 + HP15_1044 0.79 +0.1 1,065,724 - HP15_1044 0.79 -0.4 1,065,749 - HP15_1044 0.83 +1.7 1,065,846 + -0.5 1,066,331 + -0.0 1,066,372 + +0.3 1,066,372 + -0.8 1,066,391 + -0.5 1,066,399 - -0.3 1,066,427 - -0.5 1,066,509 - +0.8 1,066,516 - -0.8 1,066,582 - +0.5 1,066,599 + +1.2 1,066,635 - +0.9 1,066,641 - -0.7 1,066,711 + HP15_1047 0.12 -0.3 1,066,719 - HP15_1047 0.12 +0.1 1,066,856 + HP15_1047 0.17 -0.4 1,066,886 - HP15_1047 0.18 -1.0 1,066,892 - HP15_1047 0.19 +0.4 1,066,897 + HP15_1047 0.19 -0.2 1,066,924 - HP15_1047 0.20 -1.1 1,066,937 + HP15_1047 0.20 -0.2 1,066,955 + HP15_1047 0.21 -0.3 1,066,980 + HP15_1047 0.22 -0.7 1,067,127 - HP15_1047 0.27 -0.4 1,067,188 + HP15_1047 0.30 -1.0 1,067,234 + HP15_1047 0.32 -0.2 1,067,241 + HP15_1047 0.32 +1.1 1,067,242 + HP15_1047 0.32 -0.2 1,067,278 - HP15_1047 0.33 -0.6 1,067,320 - HP15_1047 0.35 +0.0 1,067,343 - HP15_1047 0.36 -0.5 1,067,442 + HP15_1047 0.39 -0.7 1,067,460 + +1.9 1,067,475 - HP15_1047 0.41 -0.8 1,067,475 - HP15_1047 0.41 -0.1 1,067,769 + HP15_1047 0.52 +0.7 1,067,769 + HP15_1047 0.52 -0.4 1,067,769 + HP15_1047 0.52 -0.4 1,067,769 + HP15_1047 0.52 +0.7 1,067,777 - HP15_1047 0.52 -0.3 1,067,894 + HP15_1047 0.57 +0.7 1,067,959 - HP15_1047 0.59 -0.3 1,068,033 + HP15_1047 0.62 -0.5 1,068,054 - HP15_1047 0.63 +0.3 1,068,144 + HP15_1047 0.66 -0.7 1,068,162 + HP15_1047 0.67 +0.4 1,068,164 - HP15_1047 0.67 +1.6 1,068,164 - HP15_1047 0.67 -1.2 1,068,248 + HP15_1047 0.70 -0.7 1,068,248 + HP15_1047 0.70 -0.2 1,068,606 - HP15_1047 0.84 -0.6 1,068,608 + HP15_1047 0.84 -0.6 1,068,616 - HP15_1047 0.84 -1.2 1,068,723 - HP15_1047 0.88 +1.3 1,068,793 + +0.7 1,068,877 - +0.3 1,068,877 - +0.9 1,068,897 + +0.1 1,068,907 + -0.4 1,068,915 - -0.7 1,069,038 - +0.9 1,069,118 + +0.7 1,069,394 + HP15_1046 0.55 -1.2 1,069,432 - HP15_1046 0.63 +1.6 1,069,488 + HP15_1046 0.74 +0.2
Or see this region's nucleotide sequence