Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17750

Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17745 and BWI76_RS17750 are separated by 245 nucleotidesBWI76_RS17750 and BWI76_RS17755 are separated by 130 nucleotidesBWI76_RS17755 and BWI76_RS17760 are separated by 118 nucleotides BWI76_RS17745: BWI76_RS17745 - fatty acid metabolism transcriptional regulator FadR, at 3,690,780 to 3,691,499 _RS17745 BWI76_RS17750: BWI76_RS17750 - Na+/H+ antiporter NhaB, at 3,691,745 to 3,693,298 _RS17750 BWI76_RS17755: BWI76_RS17755 - disulfide bond formation protein B, at 3,693,429 to 3,693,959 _RS17755 BWI76_RS17760: BWI76_RS17760 - hypothetical protein, at 3,694,078 to 3,694,422 _RS17760 Position (kb) 3691 3692 3693 3694Strain fitness (log2 ratio) -1 0 1at 3691.536 kb on + strandat 3691.544 kb on - strandat 3691.730 kb on + strandat 3691.761 kb on + strandat 3691.761 kb on + strandat 3692.020 kb on + strand, within BWI76_RS17750at 3692.188 kb on + strand, within BWI76_RS17750at 3692.198 kb on - strand, within BWI76_RS17750at 3692.310 kb on - strand, within BWI76_RS17750at 3692.898 kb on - strand, within BWI76_RS17750at 3692.983 kb on - strand, within BWI76_RS17750at 3693.189 kb on + strandat 3693.397 kb on + strandat 3693.420 kb on - strandat 3693.451 kb on + strandat 3693.898 kb on + strand, within BWI76_RS17755at 3694.273 kb on + strand, within BWI76_RS17760

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
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3,691,536 + -0.3
3,691,544 - -0.3
3,691,730 + -1.0
3,691,761 + +1.1
3,691,761 + -0.5
3,692,020 + BWI76_RS17750 0.18 -0.4
3,692,188 + BWI76_RS17750 0.29 -0.0
3,692,198 - BWI76_RS17750 0.29 -0.3
3,692,310 - BWI76_RS17750 0.36 -0.3
3,692,898 - BWI76_RS17750 0.74 -1.5
3,692,983 - BWI76_RS17750 0.80 -1.3
3,693,189 + -0.3
3,693,397 + +0.5
3,693,420 - -0.7
3,693,451 + -0.2
3,693,898 + BWI76_RS17755 0.88 +1.1
3,694,273 + BWI76_RS17760 0.57 -0.1

Or see this region's nucleotide sequence