Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17745 and BWI76_RS17750 are separated by 245 nucleotides BWI76_RS17750 and BWI76_RS17755 are separated by 130 nucleotides BWI76_RS17755 and BWI76_RS17760 are separated by 118 nucleotides
BWI76_RS17745: BWI76_RS17745 - fatty acid metabolism transcriptional regulator FadR, at 3,690,780 to 3,691,499
_RS17745
BWI76_RS17750: BWI76_RS17750 - Na+/H+ antiporter NhaB, at 3,691,745 to 3,693,298
_RS17750
BWI76_RS17755: BWI76_RS17755 - disulfide bond formation protein B, at 3,693,429 to 3,693,959
_RS17755
BWI76_RS17760: BWI76_RS17760 - hypothetical protein, at 3,694,078 to 3,694,422
_RS17760
Position (kb)
3691
3692
3693
3694 Strain fitness (log2 ratio)
-1
0
1 at 3691.536 kb on + strand at 3691.544 kb on - strand at 3691.730 kb on + strand at 3691.761 kb on + strand at 3691.761 kb on + strand at 3692.020 kb on + strand, within BWI76_RS17750 at 3692.188 kb on + strand, within BWI76_RS17750 at 3692.198 kb on - strand, within BWI76_RS17750 at 3692.310 kb on - strand, within BWI76_RS17750 at 3692.898 kb on - strand, within BWI76_RS17750 at 3692.983 kb on - strand, within BWI76_RS17750 at 3693.189 kb on + strand at 3693.397 kb on + strand at 3693.420 kb on - strand at 3693.451 kb on + strand at 3693.898 kb on + strand, within BWI76_RS17755 at 3694.273 kb on + strand, within BWI76_RS17760
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 3,691,536 + -0.3 3,691,544 - -0.3 3,691,730 + -1.0 3,691,761 + +1.1 3,691,761 + -0.5 3,692,020 + BWI76_RS17750 0.18 -0.4 3,692,188 + BWI76_RS17750 0.29 -0.0 3,692,198 - BWI76_RS17750 0.29 -0.3 3,692,310 - BWI76_RS17750 0.36 -0.3 3,692,898 - BWI76_RS17750 0.74 -1.5 3,692,983 - BWI76_RS17750 0.80 -1.3 3,693,189 + -0.3 3,693,397 + +0.5 3,693,420 - -0.7 3,693,451 + -0.2 3,693,898 + BWI76_RS17755 0.88 +1.1 3,694,273 + BWI76_RS17760 0.57 -0.1
Or see this region's nucleotide sequence