Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12040 and BWI76_RS12045 overlap by 4 nucleotides BWI76_RS12045 and BWI76_RS12050 overlap by 8 nucleotides BWI76_RS12050 and BWI76_RS12055 are separated by 34 nucleotides
BWI76_RS12040: BWI76_RS12040 - hypothetical protein, at 2,531,666 to 2,531,974
_RS12040
BWI76_RS12045: BWI76_RS12045 - hypothetical protein, at 2,531,971 to 2,532,690
_RS12045
BWI76_RS12050: BWI76_RS12050 - hypothetical protein, at 2,532,683 to 2,533,036
_RS12050
BWI76_RS12055: BWI76_RS12055 - DNA single-strand annealing protein RecT-like protein, at 2,533,071 to 2,534,180
_RS12055
Position (kb)
2532
2533
2534 Strain fitness (log2 ratio)
-1
0
1 at 2531.708 kb on + strand, within BWI76_RS12040 at 2531.983 kb on + strand at 2532.114 kb on - strand, within BWI76_RS12045 at 2532.233 kb on + strand, within BWI76_RS12045 at 2532.627 kb on + strand at 2532.627 kb on + strand at 2532.642 kb on + strand at 2532.645 kb on - strand at 2532.869 kb on + strand, within BWI76_RS12050 at 2532.877 kb on - strand, within BWI76_RS12050 at 2532.937 kb on + strand, within BWI76_RS12050 at 2532.956 kb on - strand at 2532.989 kb on - strand, within BWI76_RS12050 at 2533.106 kb on - strand at 2533.276 kb on + strand, within BWI76_RS12055 at 2533.531 kb on - strand, within BWI76_RS12055 at 2533.662 kb on - strand, within BWI76_RS12055 at 2533.933 kb on + strand, within BWI76_RS12055
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 2,531,708 + BWI76_RS12040 0.14 +0.1 2,531,983 + -0.4 2,532,114 - BWI76_RS12045 0.20 -0.3 2,532,233 + BWI76_RS12045 0.36 +0.2 2,532,627 + -0.1 2,532,627 + -0.1 2,532,642 + -0.6 2,532,645 - -0.4 2,532,869 + BWI76_RS12050 0.53 -0.7 2,532,877 - BWI76_RS12050 0.55 -0.5 2,532,937 + BWI76_RS12050 0.72 -0.6 2,532,956 - -0.8 2,532,989 - BWI76_RS12050 0.86 -0.6 2,533,106 - -0.5 2,533,276 + BWI76_RS12055 0.18 +0.0 2,533,531 - BWI76_RS12055 0.41 +0.0 2,533,662 - BWI76_RS12055 0.53 +0.1 2,533,933 + BWI76_RS12055 0.78 -0.4
Or see this region's nucleotide sequence