Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09555 and BWI76_RS09560 are separated by 151 nucleotides BWI76_RS09560 and BWI76_RS09565 are separated by 39 nucleotides BWI76_RS09565 and BWI76_RS09570 are separated by 11 nucleotides
BWI76_RS09555: BWI76_RS09555 - pyruvate oxidase, at 1,998,424 to 2,000,142
_RS09555
BWI76_RS09560: BWI76_RS09560 - DoxX family membrane protein, at 2,000,294 to 2,000,728
_RS09560
BWI76_RS09565: BWI76_RS09565 - hybrid-cluster NAD(P)-dependent oxidoreductase, at 2,000,768 to 2,001,736
_RS09565
BWI76_RS09570: BWI76_RS09570 - hydroxylamine reductase, at 2,001,748 to 2,003,400
_RS09570
Position (kb)
2000
2001
2002 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2000.108 kb on - strand at 2000.178 kb on + strand at 2000.441 kb on - strand, within BWI76_RS09560 at 2000.602 kb on - strand, within BWI76_RS09560 at 2000.916 kb on - strand, within BWI76_RS09565 at 2000.965 kb on + strand, within BWI76_RS09565 at 2001.280 kb on + strand, within BWI76_RS09565 at 2001.288 kb on - strand, within BWI76_RS09565 at 2001.446 kb on - strand, within BWI76_RS09565 at 2001.562 kb on + strand, within BWI76_RS09565 at 2001.966 kb on + strand, within BWI76_RS09570 at 2002.022 kb on + strand, within BWI76_RS09570 at 2002.096 kb on - strand, within BWI76_RS09570 at 2002.134 kb on - strand, within BWI76_RS09570 at 2002.249 kb on - strand, within BWI76_RS09570 at 2002.592 kb on + strand, within BWI76_RS09570 at 2002.643 kb on + strand, within BWI76_RS09570 at 2002.668 kb on + strand, within BWI76_RS09570
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 2,000,108 - +0.2 2,000,178 + +1.2 2,000,441 - BWI76_RS09560 0.34 -0.8 2,000,602 - BWI76_RS09560 0.71 -1.7 2,000,916 - BWI76_RS09565 0.15 -0.3 2,000,965 + BWI76_RS09565 0.20 +0.1 2,001,280 + BWI76_RS09565 0.53 -0.6 2,001,288 - BWI76_RS09565 0.54 -0.1 2,001,446 - BWI76_RS09565 0.70 -0.1 2,001,562 + BWI76_RS09565 0.82 +0.3 2,001,966 + BWI76_RS09570 0.13 +0.7 2,002,022 + BWI76_RS09570 0.17 -0.0 2,002,096 - BWI76_RS09570 0.21 -0.6 2,002,134 - BWI76_RS09570 0.23 +0.4 2,002,249 - BWI76_RS09570 0.30 +0.3 2,002,592 + BWI76_RS09570 0.51 +0.4 2,002,643 + BWI76_RS09570 0.54 +2.8 2,002,668 + BWI76_RS09570 0.56 -0.1
Or see this region's nucleotide sequence