Experiment: LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02805 and BWI76_RS02810 overlap by 14 nucleotides BWI76_RS02810 and BWI76_RS02815 are separated by 44 nucleotides
BWI76_RS02805: BWI76_RS02805 - sugar ABC transporter permease, at 572,805 to 573,830
_RS02805
BWI76_RS02810: BWI76_RS02810 - sugar ABC transporter permease YjfF, at 573,817 to 574,803
_RS02810
BWI76_RS02815: BWI76_RS02815 - fructose-bisphosphatase class I, at 574,848 to 575,846
_RS02815
Position (kb)
573
574
575 Strain fitness (log2 ratio)
-1
0
1 at 572.865 kb on - strand at 573.046 kb on + strand, within BWI76_RS02805 at 573.263 kb on + strand, within BWI76_RS02805 at 573.268 kb on + strand, within BWI76_RS02805 at 573.526 kb on + strand, within BWI76_RS02805 at 573.558 kb on + strand, within BWI76_RS02805 at 573.881 kb on - strand at 574.280 kb on - strand, within BWI76_RS02810 at 574.280 kb on - strand, within BWI76_RS02810 at 574.549 kb on - strand, within BWI76_RS02810 at 574.598 kb on + strand, within BWI76_RS02810 at 574.690 kb on - strand, within BWI76_RS02810 at 574.892 kb on - strand at 575.069 kb on + strand, within BWI76_RS02815 at 575.351 kb on - strand, within BWI76_RS02815 at 575.631 kb on - strand, within BWI76_RS02815 at 575.769 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x remove 572,865 - +0.2 573,046 + BWI76_RS02805 0.23 -0.4 573,263 + BWI76_RS02805 0.45 -1.0 573,268 + BWI76_RS02805 0.45 +0.6 573,526 + BWI76_RS02805 0.70 -0.3 573,558 + BWI76_RS02805 0.73 +0.1 573,881 - +0.2 574,280 - BWI76_RS02810 0.47 +0.6 574,280 - BWI76_RS02810 0.47 -0.6 574,549 - BWI76_RS02810 0.74 -0.3 574,598 + BWI76_RS02810 0.79 -0.8 574,690 - BWI76_RS02810 0.88 -0.6 574,892 - -0.2 575,069 + BWI76_RS02815 0.22 +0.1 575,351 - BWI76_RS02815 0.50 -0.9 575,631 - BWI76_RS02815 0.78 +0.1 575,769 + -0.2
Or see this region's nucleotide sequence