Experiment: Protocatechuic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23180 and RR42_RS23185 are separated by 233 nucleotides RR42_RS23185 and RR42_RS23190 are separated by 120 nucleotides RR42_RS23190 and RR42_RS23195 are separated by 186 nucleotides
RR42_RS23180: RR42_RS23180 - ligand-gated channel, at 533,167 to 535,353
_RS23180
RR42_RS23185: RR42_RS23185 - membrane protein, at 535,587 to 536,636
_RS23185
RR42_RS23190: RR42_RS23190 - membrane protein, at 536,757 to 537,284
_RS23190
RR42_RS23195: RR42_RS23195 - membrane protein, at 537,471 to 538,349
_RS23195
Position (kb)
535
536
537 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 534.628 kb on + strand, within RR42_RS23180 at 534.628 kb on + strand, within RR42_RS23180 at 534.628 kb on + strand, within RR42_RS23180 at 534.703 kb on + strand, within RR42_RS23180 at 534.704 kb on - strand, within RR42_RS23180 at 534.766 kb on + strand, within RR42_RS23180 at 534.766 kb on + strand, within RR42_RS23180 at 534.767 kb on - strand, within RR42_RS23180 at 534.790 kb on + strand, within RR42_RS23180 at 534.802 kb on + strand, within RR42_RS23180 at 534.836 kb on + strand, within RR42_RS23180 at 534.836 kb on + strand, within RR42_RS23180 at 534.837 kb on - strand, within RR42_RS23180 at 534.869 kb on - strand, within RR42_RS23180 at 534.914 kb on - strand, within RR42_RS23180 at 535.054 kb on + strand, within RR42_RS23180 at 535.054 kb on + strand, within RR42_RS23180 at 535.054 kb on + strand, within RR42_RS23180 at 535.055 kb on - strand, within RR42_RS23180 at 535.158 kb on + strand at 535.165 kb on + strand at 535.165 kb on + strand at 535.165 kb on + strand at 535.166 kb on - strand at 535.205 kb on - strand at 535.281 kb on + strand at 535.288 kb on + strand at 535.289 kb on - strand at 535.289 kb on - strand at 535.351 kb on + strand at 535.352 kb on - strand at 535.352 kb on - strand at 535.421 kb on + strand at 535.512 kb on + strand at 535.512 kb on + strand at 535.513 kb on - strand at 535.513 kb on - strand at 535.513 kb on - strand at 535.513 kb on - strand at 535.610 kb on + strand at 535.826 kb on - strand, within RR42_RS23185 at 535.826 kb on - strand, within RR42_RS23185 at 535.826 kb on - strand, within RR42_RS23185 at 535.900 kb on + strand, within RR42_RS23185 at 535.900 kb on + strand, within RR42_RS23185 at 535.901 kb on - strand, within RR42_RS23185 at 536.078 kb on + strand, within RR42_RS23185 at 536.109 kb on + strand, within RR42_RS23185 at 536.109 kb on + strand, within RR42_RS23185 at 536.109 kb on + strand, within RR42_RS23185 at 536.110 kb on - strand, within RR42_RS23185 at 536.518 kb on + strand, within RR42_RS23185 at 536.519 kb on - strand, within RR42_RS23185 at 536.700 kb on + strand at 536.700 kb on + strand at 536.700 kb on + strand at 536.700 kb on + strand at 536.701 kb on - strand at 536.701 kb on - strand at 536.702 kb on + strand at 536.702 kb on + strand at 536.702 kb on + strand at 536.703 kb on - strand at 536.703 kb on - strand at 536.704 kb on + strand at 536.704 kb on + strand at 536.704 kb on + strand at 536.704 kb on + strand at 536.704 kb on + strand at 536.704 kb on + strand at 536.704 kb on + strand at 536.704 kb on + strand at 536.705 kb on - strand at 536.705 kb on - strand at 536.705 kb on - strand at 536.705 kb on - strand at 536.757 kb on - strand at 536.775 kb on + strand at 536.915 kb on + strand, within RR42_RS23190 at 536.915 kb on + strand, within RR42_RS23190 at 536.915 kb on + strand, within RR42_RS23190 at 536.916 kb on - strand, within RR42_RS23190 at 536.916 kb on - strand, within RR42_RS23190 at 536.916 kb on - strand, within RR42_RS23190 at 536.920 kb on - strand, within RR42_RS23190 at 536.952 kb on + strand, within RR42_RS23190 at 536.973 kb on + strand, within RR42_RS23190 at 536.973 kb on + strand, within RR42_RS23190 at 537.052 kb on - strand, within RR42_RS23190 at 537.052 kb on - strand, within RR42_RS23190 at 537.052 kb on - strand, within RR42_RS23190 at 537.068 kb on + strand, within RR42_RS23190 at 537.068 kb on + strand, within RR42_RS23190 at 537.126 kb on + strand, within RR42_RS23190 at 537.127 kb on - strand, within RR42_RS23190 at 537.127 kb on - strand, within RR42_RS23190 at 537.127 kb on - strand, within RR42_RS23190 at 537.160 kb on - strand, within RR42_RS23190 at 537.160 kb on - strand, within RR42_RS23190 at 537.160 kb on - strand, within RR42_RS23190 at 537.160 kb on - strand, within RR42_RS23190 at 537.513 kb on + strand at 537.513 kb on + strand at 537.513 kb on + strand at 537.514 kb on - strand at 537.514 kb on - strand at 537.514 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Protocatechuic Acid (C) remove 534,628 + RR42_RS23180 0.67 -1.8 534,628 + RR42_RS23180 0.67 +0.5 534,628 + RR42_RS23180 0.67 +1.4 534,703 + RR42_RS23180 0.70 -1.2 534,704 - RR42_RS23180 0.70 -1.0 534,766 + RR42_RS23180 0.73 -0.1 534,766 + RR42_RS23180 0.73 +1.1 534,767 - RR42_RS23180 0.73 +1.6 534,790 + RR42_RS23180 0.74 -1.2 534,802 + RR42_RS23180 0.75 -0.7 534,836 + RR42_RS23180 0.76 -0.1 534,836 + RR42_RS23180 0.76 -0.5 534,837 - RR42_RS23180 0.76 -0.2 534,869 - RR42_RS23180 0.78 +2.3 534,914 - RR42_RS23180 0.80 +0.5 535,054 + RR42_RS23180 0.86 -0.9 535,054 + RR42_RS23180 0.86 -0.8 535,054 + RR42_RS23180 0.86 +0.3 535,055 - RR42_RS23180 0.86 -2.1 535,158 + +0.8 535,165 + +1.1 535,165 + +0.5 535,165 + -1.9 535,166 - -0.1 535,205 - +0.6 535,281 + +0.5 535,288 + +0.1 535,289 - +0.5 535,289 - -0.3 535,351 + +2.5 535,352 - -0.7 535,352 - +0.9 535,421 + +0.1 535,512 + +0.0 535,512 + +1.4 535,513 - -1.2 535,513 - -0.6 535,513 - +0.2 535,513 - -2.4 535,610 + -2.5 535,826 - RR42_RS23185 0.23 +0.7 535,826 - RR42_RS23185 0.23 +0.9 535,826 - RR42_RS23185 0.23 +0.3 535,900 + RR42_RS23185 0.30 +1.5 535,900 + RR42_RS23185 0.30 -1.0 535,901 - RR42_RS23185 0.30 +1.1 536,078 + RR42_RS23185 0.47 -1.1 536,109 + RR42_RS23185 0.50 -1.3 536,109 + RR42_RS23185 0.50 +0.9 536,109 + RR42_RS23185 0.50 -0.9 536,110 - RR42_RS23185 0.50 +1.5 536,518 + RR42_RS23185 0.89 -0.8 536,519 - RR42_RS23185 0.89 -0.5 536,700 + -0.2 536,700 + +2.8 536,700 + +0.4 536,700 + -0.4 536,701 - -0.6 536,701 - +0.5 536,702 + -0.4 536,702 + +0.2 536,702 + -0.1 536,703 - +0.5 536,703 - -0.7 536,704 + -2.9 536,704 + +1.2 536,704 + +0.5 536,704 + -0.4 536,704 + +0.7 536,704 + +2.1 536,704 + -0.4 536,704 + -0.3 536,705 - -1.6 536,705 - -2.9 536,705 - -0.5 536,705 - -1.3 536,757 - +0.3 536,775 + +0.9 536,915 + RR42_RS23190 0.30 -1.6 536,915 + RR42_RS23190 0.30 +0.1 536,915 + RR42_RS23190 0.30 -0.9 536,916 - RR42_RS23190 0.30 +0.9 536,916 - RR42_RS23190 0.30 +1.8 536,916 - RR42_RS23190 0.30 +1.3 536,920 - RR42_RS23190 0.31 -0.3 536,952 + RR42_RS23190 0.37 -0.8 536,973 + RR42_RS23190 0.41 -0.5 536,973 + RR42_RS23190 0.41 -0.7 537,052 - RR42_RS23190 0.56 +1.1 537,052 - RR42_RS23190 0.56 +0.8 537,052 - RR42_RS23190 0.56 +1.5 537,068 + RR42_RS23190 0.59 +0.1 537,068 + RR42_RS23190 0.59 +2.1 537,126 + RR42_RS23190 0.70 -0.2 537,127 - RR42_RS23190 0.70 -1.2 537,127 - RR42_RS23190 0.70 -0.4 537,127 - RR42_RS23190 0.70 -1.5 537,160 - RR42_RS23190 0.76 -1.2 537,160 - RR42_RS23190 0.76 -0.8 537,160 - RR42_RS23190 0.76 -0.3 537,160 - RR42_RS23190 0.76 +0.2 537,513 + +2.2 537,513 + +0.5 537,513 + +1.6 537,514 - -0.2 537,514 - -1.0 537,514 - +1.4
Or see this region's nucleotide sequence