Experiment: Protocatechuic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS10640 and RR42_RS10645 are separated by 154 nucleotides RR42_RS10645 and RR42_RS10650 are separated by 177 nucleotides RR42_RS10650 and RR42_RS10655 overlap by 4 nucleotides
RR42_RS10640: RR42_RS10640 - alanine dehydrogenase, at 2,273,201 to 2,274,316
_RS10640
RR42_RS10645: RR42_RS10645 - hypothetical protein, at 2,274,471 to 2,274,818
_RS10645
RR42_RS10650: RR42_RS10650 - acetyl-CoA synthetase, at 2,274,996 to 2,276,762
_RS10650
RR42_RS10655: RR42_RS10655 - pyruvate dehydrogenase, at 2,276,759 to 2,277,781
_RS10655
Position (kb)
2274
2275
2276
2277 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2274.037 kb on - strand, within RR42_RS10640 at 2274.037 kb on - strand, within RR42_RS10640 at 2274.043 kb on - strand, within RR42_RS10640 at 2274.125 kb on + strand, within RR42_RS10640 at 2274.146 kb on + strand, within RR42_RS10640 at 2274.146 kb on + strand, within RR42_RS10640 at 2274.147 kb on - strand, within RR42_RS10640 at 2274.169 kb on - strand, within RR42_RS10640 at 2274.251 kb on - strand at 2274.472 kb on + strand at 2274.472 kb on + strand at 2274.473 kb on - strand at 2274.549 kb on + strand, within RR42_RS10645 at 2274.549 kb on + strand, within RR42_RS10645 at 2274.654 kb on + strand, within RR42_RS10645 at 2274.654 kb on + strand, within RR42_RS10645 at 2274.655 kb on - strand, within RR42_RS10645 at 2274.655 kb on - strand, within RR42_RS10645 at 2274.656 kb on + strand, within RR42_RS10645 at 2274.657 kb on - strand, within RR42_RS10645 at 2274.657 kb on - strand, within RR42_RS10645 at 2274.751 kb on - strand, within RR42_RS10645 at 2274.751 kb on - strand, within RR42_RS10645 at 2274.767 kb on - strand, within RR42_RS10645 at 2274.767 kb on - strand, within RR42_RS10645 at 2274.894 kb on - strand at 2274.894 kb on - strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.895 kb on + strand at 2274.896 kb on - strand at 2274.959 kb on - strand at 2274.966 kb on + strand at 2274.967 kb on - strand at 2274.967 kb on - strand at 2275.175 kb on + strand, within RR42_RS10650 at 2275.239 kb on + strand, within RR42_RS10650 at 2275.348 kb on - strand, within RR42_RS10650 at 2275.654 kb on + strand, within RR42_RS10650 at 2275.655 kb on - strand, within RR42_RS10650 at 2275.773 kb on + strand, within RR42_RS10650 at 2275.773 kb on + strand, within RR42_RS10650 at 2275.773 kb on + strand, within RR42_RS10650 at 2275.782 kb on - strand, within RR42_RS10650 at 2275.815 kb on + strand, within RR42_RS10650 at 2275.890 kb on + strand, within RR42_RS10650 at 2275.926 kb on + strand, within RR42_RS10650 at 2275.926 kb on + strand, within RR42_RS10650 at 2275.986 kb on + strand, within RR42_RS10650 at 2276.228 kb on - strand, within RR42_RS10650 at 2276.228 kb on - strand, within RR42_RS10650 at 2276.230 kb on + strand, within RR42_RS10650 at 2276.231 kb on - strand, within RR42_RS10650 at 2276.231 kb on - strand, within RR42_RS10650 at 2276.382 kb on + strand, within RR42_RS10650 at 2276.383 kb on - strand, within RR42_RS10650 at 2276.386 kb on - strand, within RR42_RS10650 at 2276.386 kb on - strand, within RR42_RS10650 at 2276.386 kb on - strand, within RR42_RS10650 at 2276.518 kb on + strand, within RR42_RS10650 at 2276.519 kb on - strand, within RR42_RS10650 at 2276.736 kb on - strand at 2276.816 kb on - strand at 2276.889 kb on - strand, within RR42_RS10655 at 2276.904 kb on + strand, within RR42_RS10655 at 2276.905 kb on - strand, within RR42_RS10655 at 2276.924 kb on + strand, within RR42_RS10655 at 2276.924 kb on + strand, within RR42_RS10655 at 2276.925 kb on - strand, within RR42_RS10655 at 2276.996 kb on + strand, within RR42_RS10655 at 2276.997 kb on - strand, within RR42_RS10655 at 2277.059 kb on + strand, within RR42_RS10655 at 2277.395 kb on + strand, within RR42_RS10655 at 2277.396 kb on - strand, within RR42_RS10655 at 2277.401 kb on - strand, within RR42_RS10655 at 2277.401 kb on - strand, within RR42_RS10655 at 2277.401 kb on - strand, within RR42_RS10655 at 2277.515 kb on + strand, within RR42_RS10655 at 2277.516 kb on - strand, within RR42_RS10655 at 2277.540 kb on - strand, within RR42_RS10655 at 2277.554 kb on + strand, within RR42_RS10655 at 2277.604 kb on + strand, within RR42_RS10655 at 2277.605 kb on - strand, within RR42_RS10655 at 2277.605 kb on - strand, within RR42_RS10655 at 2277.755 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Protocatechuic Acid (C) remove 2,274,037 - RR42_RS10640 0.75 -2.7 2,274,037 - RR42_RS10640 0.75 -1.0 2,274,043 - RR42_RS10640 0.75 -0.8 2,274,125 + RR42_RS10640 0.83 -1.0 2,274,146 + RR42_RS10640 0.85 -1.0 2,274,146 + RR42_RS10640 0.85 +0.2 2,274,147 - RR42_RS10640 0.85 -1.2 2,274,169 - RR42_RS10640 0.87 +0.4 2,274,251 - +0.1 2,274,472 + -2.0 2,274,472 + -2.7 2,274,473 - -0.5 2,274,549 + RR42_RS10645 0.22 -0.6 2,274,549 + RR42_RS10645 0.22 -0.6 2,274,654 + RR42_RS10645 0.53 -1.0 2,274,654 + RR42_RS10645 0.53 -1.1 2,274,655 - RR42_RS10645 0.53 +0.1 2,274,655 - RR42_RS10645 0.53 -2.7 2,274,656 + RR42_RS10645 0.53 -3.2 2,274,657 - RR42_RS10645 0.53 -0.5 2,274,657 - RR42_RS10645 0.53 +0.2 2,274,751 - RR42_RS10645 0.80 +0.9 2,274,751 - RR42_RS10645 0.80 +0.4 2,274,767 - RR42_RS10645 0.85 -1.7 2,274,767 - RR42_RS10645 0.85 -0.8 2,274,894 - -2.1 2,274,894 - +1.0 2,274,895 + +0.5 2,274,895 + -1.3 2,274,895 + +0.3 2,274,895 + +0.8 2,274,895 + +1.5 2,274,895 + +0.0 2,274,896 - -0.8 2,274,959 - -0.3 2,274,966 + +0.4 2,274,967 - -2.9 2,274,967 - -2.3 2,275,175 + RR42_RS10650 0.10 +0.8 2,275,239 + RR42_RS10650 0.14 -0.2 2,275,348 - RR42_RS10650 0.20 +0.5 2,275,654 + RR42_RS10650 0.37 +0.1 2,275,655 - RR42_RS10650 0.37 +0.8 2,275,773 + RR42_RS10650 0.44 -1.1 2,275,773 + RR42_RS10650 0.44 +0.1 2,275,773 + RR42_RS10650 0.44 +0.8 2,275,782 - RR42_RS10650 0.44 +1.1 2,275,815 + RR42_RS10650 0.46 -0.0 2,275,890 + RR42_RS10650 0.51 +0.8 2,275,926 + RR42_RS10650 0.53 +0.0 2,275,926 + RR42_RS10650 0.53 -0.4 2,275,986 + RR42_RS10650 0.56 -0.5 2,276,228 - RR42_RS10650 0.70 +1.0 2,276,228 - RR42_RS10650 0.70 -0.2 2,276,230 + RR42_RS10650 0.70 -1.1 2,276,231 - RR42_RS10650 0.70 -1.6 2,276,231 - RR42_RS10650 0.70 +0.2 2,276,382 + RR42_RS10650 0.78 +2.3 2,276,383 - RR42_RS10650 0.78 +0.5 2,276,386 - RR42_RS10650 0.79 +2.3 2,276,386 - RR42_RS10650 0.79 +0.6 2,276,386 - RR42_RS10650 0.79 -0.4 2,276,518 + RR42_RS10650 0.86 +0.6 2,276,519 - RR42_RS10650 0.86 -0.1 2,276,736 - -0.7 2,276,816 - -1.8 2,276,889 - RR42_RS10655 0.13 +0.2 2,276,904 + RR42_RS10655 0.14 -1.1 2,276,905 - RR42_RS10655 0.14 +0.4 2,276,924 + RR42_RS10655 0.16 +1.1 2,276,924 + RR42_RS10655 0.16 -0.0 2,276,925 - RR42_RS10655 0.16 +0.2 2,276,996 + RR42_RS10655 0.23 +0.8 2,276,997 - RR42_RS10655 0.23 -2.0 2,277,059 + RR42_RS10655 0.29 -0.3 2,277,395 + RR42_RS10655 0.62 -0.3 2,277,396 - RR42_RS10655 0.62 -0.3 2,277,401 - RR42_RS10655 0.63 +1.5 2,277,401 - RR42_RS10655 0.63 +0.2 2,277,401 - RR42_RS10655 0.63 -0.4 2,277,515 + RR42_RS10655 0.74 -1.0 2,277,516 - RR42_RS10655 0.74 +0.8 2,277,540 - RR42_RS10655 0.76 +0.6 2,277,554 + RR42_RS10655 0.78 -0.0 2,277,604 + RR42_RS10655 0.83 -0.8 2,277,605 - RR42_RS10655 0.83 +0.1 2,277,605 - RR42_RS10655 0.83 +0.7 2,277,755 + +0.0
Or see this region's nucleotide sequence