Experiment: LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20240 and BWI76_RS20245 are separated by 46 nucleotides BWI76_RS20245 and BWI76_RS20250 overlap by 8 nucleotides BWI76_RS20250 and BWI76_RS20255 overlap by 4 nucleotides
BWI76_RS20240: BWI76_RS20240 - phosphate acetyltransferase, at 4,241,656 to 4,243,800
_RS20240
BWI76_RS20245: BWI76_RS20245 - transketolase subunit B, at 4,243,847 to 4,244,800
_RS20245
BWI76_RS20250: BWI76_RS20250 - hypothetical protein, at 4,244,793 to 4,245,623
_RS20250
BWI76_RS20255: BWI76_RS20255 - PTS ascorbate transporter subunit IIC, at 4,245,620 to 4,247,011
_RS20255
Position (kb) 4243
4244
4245 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4242.998 kb on + strand, within BWI76_RS20240 at 4243.710 kb on + strand at 4243.750 kb on + strand at 4243.928 kb on - strand at 4243.942 kb on + strand at 4243.976 kb on + strand, within BWI76_RS20245 at 4244.137 kb on - strand, within BWI76_RS20245 at 4244.455 kb on + strand, within BWI76_RS20245 at 4244.507 kb on - strand, within BWI76_RS20245 at 4244.668 kb on + strand, within BWI76_RS20245 at 4244.742 kb on + strand at 4244.791 kb on + strand at 4244.901 kb on + strand, within BWI76_RS20250 at 4245.094 kb on - strand, within BWI76_RS20250 at 4245.094 kb on - strand, within BWI76_RS20250 at 4245.290 kb on + strand, within BWI76_RS20250 at 4245.355 kb on - strand, within BWI76_RS20250 at 4245.434 kb on + strand, within BWI76_RS20250 at 4245.462 kb on - strand, within BWI76_RS20250 at 4245.633 kb on + strand at 4245.638 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 4,242,998 + BWI76_RS20240 0.63 -1.2 4,243,710 + -0.6 4,243,750 + -1.4 4,243,928 - -0.5 4,243,942 + +0.4 4,243,976 + BWI76_RS20245 0.14 -1.2 4,244,137 - BWI76_RS20245 0.30 -0.8 4,244,455 + BWI76_RS20245 0.64 +0.1 4,244,507 - BWI76_RS20245 0.69 -1.1 4,244,668 + BWI76_RS20245 0.86 +2.0 4,244,742 + +0.2 4,244,791 + -0.0 4,244,901 + BWI76_RS20250 0.13 -0.2 4,245,094 - BWI76_RS20250 0.36 -0.4 4,245,094 - BWI76_RS20250 0.36 -1.9 4,245,290 + BWI76_RS20250 0.60 -0.0 4,245,355 - BWI76_RS20250 0.68 +0.6 4,245,434 + BWI76_RS20250 0.77 +0.7 4,245,462 - BWI76_RS20250 0.81 -0.0 4,245,633 + -0.4 4,245,638 + +0.6
Or see this region's nucleotide sequence