Experiment: LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08115 and BWI76_RS08120 are separated by 22 nucleotides BWI76_RS08120 and BWI76_RS08125 are separated by 98 nucleotides BWI76_RS08125 and BWI76_RS08130 overlap by 4 nucleotides
BWI76_RS08115: BWI76_RS08115 - apolipoprotein N-acyltransferase, at 1,719,938 to 1,721,476
_RS08115
BWI76_RS08120: BWI76_RS08120 - cobalt transporter, at 1,721,499 to 1,722,377
_RS08120
BWI76_RS08125: BWI76_RS08125 - rRNA maturation RNase YbeY, at 1,722,476 to 1,722,943
_RS08125
BWI76_RS08130: BWI76_RS08130 - nucleoside triphosphate hydrolase, at 1,722,940 to 1,723,986
_RS08130
Position (kb) 1721
1722
1723 Strain fitness (log2 ratio) -1
0
1 at 1721.755 kb on - strand, within BWI76_RS08120 at 1721.839 kb on - strand, within BWI76_RS08120 at 1722.199 kb on - strand, within BWI76_RS08120 at 1722.482 kb on - strand at 1723.209 kb on - strand, within BWI76_RS08130
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 1,721,755 - BWI76_RS08120 0.29 -0.7 1,721,839 - BWI76_RS08120 0.39 -1.0 1,722,199 - BWI76_RS08120 0.80 -0.1 1,722,482 - -0.8 1,723,209 - BWI76_RS08130 0.26 +0.3
Or see this region's nucleotide sequence